cDNA Clone PIM2

• Plasmid: pEBG6P-1 PIM2 ctg ORF D241A (Kdead)
• Parent Plasmid: pEBG6P
• Expressed: GST-PIM2 ctg ORF D241A (Kdead) (NM_006875.2 )
• Genbank: NM_006875.2 GI:42821111 (N.B. This record replaced NM_006875.1 GI:5803124 on 26-FEB-2004)
• Tag: GST
• Species: Human

Sequence of Insert

ggatccCTGGCGCGCGCGGCGAATCTCAACGCTGCGCCGTCTGCGGGCGCTTCCGGGCCACCAGTTTCTCTGCTTTCCACCCTGGCGCCCCCCAGCCCTGGCTCCCCAGCTGCGCTGCCCCGGGCGTCCACGCCCTGCGGGCTTAGCGGGTTCAGTGGGCTCAATCTGCGCAGCGCCACCTCCATGTTGACCAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAAGGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAGGGG
GGCTTTGGCACCGTCTTCGCAGGACACCGCCTCACAGATCGACTCCAGGT
GGCCATCAAAGTGATTCCCCGGAATCGTGTGCTGGGCTGGTCCCCCTTGT
CAGACTCAGTCACATGCCCACTCGAAGTCGCACTGCTATGGAAAGTGGGT
GCAGGTGGTGGGCACCCTGGCGTGATCCGCCTGCTTGACTGGTTTGAGAC
ACAGGAGGGCTTCATGCTGGTCCTCGAGCGGCCTTTGCCCGCCCAGGATC
TCTTTGACTATATCACAGAGAAGGGCCCACTGGGTGAAGGCCCAAGCCGC
TGCTTCTTTGGCCAAGTAGTGGCAGCCATCCAGCACTGCCATTCCCGTGG
AGTTGTCCATCGTGACATCAAGGATGAGAACATCCTGATAGACCTACGCC
GTGGCTGTGCCAAACTCATTGCTTTTGGTTCTGGTGCCCTGCTTCATGAT
GAACCCTACACTGACTTTGATGGGACAAGGGTGTACAGCCCCCCAGAGTG
GATCTCTCGACACCAGTACCATGCACTCCCGGCCACTGTCTGGTCACTGG
GCATCCTCCTCTATGACATGGTGTGTGGGGACATTCCCTTTGAGAGGGAC
CAGGAGATTCTGGAAGCTGAGCTCCACTTCCCAGCCCATGTCTCCCCAGA
CTGCTGTGCCCTAATCCGCCGGTGCCTGGCCCCCAAACCTTCTTCCCGAC
CCTCACTGGAAGAGATCCTGCTGGACCCCTGGATGCAAACACCAGCCGAG
GATGTACCCCTCAACCCCTCCAAAGGAGGCCCTGCCCCTTTGGCCTGGTC
CTTGCTACCCTAAGAATTCCCGGGTCGACTCGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSLARAANLNAAPSA
GASGPPVSLLSTLAPPSPGSPAALPRASTPCGLSGFSGLNLRSATSMLTK
PLQGPPAPPGTPTPPPGGKDREAFEAEYRLGPLLGKGGFGTVFAGHRLTD
RLQVAIKVIPRNRVLGWSPLSDSVTCPLEVALLWKVGAGGGHPGVIRLLD
WFETQEGFMLVLERPLPAQDLFDYITEKGPLGEGPSRCFFGQVVAAIQHC
HSRGVVHRDIKDENILIDLRRGCAKLIAFGSGALLHDEPYTDFDGTRVYS
PPEWISRHQYHALPATVWSLGILLYDMVCGDIPFERDQEILEAELHFPAH
VSPDCCALIRRCLAPKPSSRPSLEEILLDPWMQTPAEDVPLNPSKGGPAP
LAWSLLP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5239
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 67046 Daltons