cDNA Clone glucose oxidase

• Plasmid: pRB1438 Glucose oxidase (1-605).6His
• Parent Plasmid: pRB1438
• Tag: 6His
• Species: Aspergillus niger

Sequence of Insert

aagcttCTAACCATGCAGACTCTCCTTGTGAGCTCGCTTGTGGTCTCCATTGCCACCGCCCTGCCACACTACATCAGGAGCAATGGCATTGAAGCAAACCTCCTGACCGATCCCAACGATGTCGCCGGCCGCACCGTCGACTACATCATCGCTGGTGGAGGTCTGACTGGGCTCACCACCGCCGCCCGTCTGACTGAGGACCCCAACATCACCGTCCTCGTCATTGAAAGTGGCTCCTACGAGTCAGACAGGGGTGCTATCATCGAGGACCTCAACGCCTACGGCGACATCTTTGGCAGCAGCGTCGACCATGCCTACGAGACCGTGACGCTCGCCACCAACAATCAGACTGCGCTGATCCGCTCCGGAAATGGTCTCGGTGGCTCTACCTTGGTCAATGGTGGCACTTGGACTCGCCCCCACAAAGCGCAAGTTGACTCATGGGAGACTGTCTTTGGCAATGAGGGCTGGAACTGGGACAACGTGGCTGCCTACTCCCTCCAGGCGGAGCGTGCTCGCGCACCCAACGCCAAACAGATCGCTGCTGGCCACTACTTCAACGCATCCTGTCATGGTGTCAACGGCACTGTCCATGCCGGACCCCGCGACACC
GGCGATGACTATTCTCCCATCGTCCAGGCTCTCATGAGCGCTGTGGAAGA
CAGGGGCGTCCCTACCAAGAAGGACTTTGGATGCGGTGACCCCCATGGTG
TGTCCATGTTCCCCAACACCTTGCACGAAGACCAAGTGCGCTCCGATGCC
GCTCGCGAATGGCTCCTTCCCAACTACCAGCGTCCCAACTTGCAAGTCCT
GACCGGACAGTTTGTCGGAAAGGTCTTGCTCAACCAGAACACGACCACCC
CTCGTGCCGTCGGTGTGGAATTCGGCACGCACAAGGGCAACACCCACAAC
GTCTACGCGAAGCACGAGGTCCTCCTGGCCGCTGGCTCCGCTATCTCTCC
CACCATCCTCGAATATTCCGGTATCGGCATGAAGTCCATCCTGGAGCCTC
TTGGAATCGATACCGTCGTTGACCTTCCTGTCGGCCTGAACCTGCAGGAC
CAGACTACTGCTACCGTCCGCTCGCGCATCACCTCCGCCGGTGCCGGACA
GGGACAGGCTGCTTGGTTCGCCACCTTCAACGAGACCTTTGGTGACTACG
CCGAAAAGGCGCATGAGCTGCTGAACACCAAGCTGGAGCAGTGGGCCGAA
GAGGCCGTCGCCCGTGGCGGATTCCACAACACCACCGCCTTGCTCATCCA
GTACGAGAACTACCGCGACTGGATCGTCAACCACAATGTCGCGTACGCGG
AACTTTTCCTCGACACGGCCGGAGTGGCCAGCTTTGACGTCTGGGACCTC
CTTCCCTTCACGAGAGGATACGTCCATATTCTCGACAAGGACCCCTACCT
CCGCCACTTCGCCTACGACCCTCAGTACTTCCTCAACGAGCTGGACCTGC
TCGGTCAGGCGTCCGCTCTTCAGCTGGCCCGGAACATCTCCAACTCGGGT
GCCATGCAGACGTACTTCGCAGGAGAGACCATTCCCGGTGATAACCTCGC
GTATGATGCCGATTTGATGGCCTGGGCTGAGTATCTCCCGTACCACTTCC
GTCCCAACTACCATGGAGTGGGAACGTGCTCCATGATGTCGAGGGAGATG
GGCGGTGTGGTCGACAATGCTGCCCGTGTGTATGGTGTGCAGGGGCTGCG
GGTCATTGATGGTTCTATTCCGCCTACGCAGGTCTCGTCCCATGTGATGA
CGGTGTTTTATGCCATGGCGCTGAAGATTGCGGACGCTATCTTGGAAGAT
TATGCTTCCATGCAGCATCACCATCACCATCACTGAggtacc

Amino Acid Sequence

MQTLLVSSLVVSIATALPHYIRSNGIEANLLTDPNDVAGRTVDYIIAGGG
LTGLTTAARLTEDPNITVLVIESGSYESDRGAIIEDLNAYGDIFGSSVDH
AYETVTLATNNQTALIRSGNGLGGSTLVNGGTWTRPHKAQVDSWETVFGN
EGWNWDNVAAYSLQAERARAPNAKQIAAGHYFNASCHGVNGTVHAGPRDT
GDDYSPIVQALMSAVEDRGVPTKKDFGCGDPHGVSMFPNTLHEDQVRSDA
AREWLLPNYQRPNLQVLTGQFVGKVLLNQNTTTPRAVGVEFGTHKGNTHN
VYAKHEVLLAAGSAISPTILEYSGIGMKSILEPLGIDTVVDLPVGLNLQD
QTTATVRSRITSAGAGQGQAAWFATFNETFGDYAEKAHELLNTKLEQWAE
EAVARGGFHNTTALLIQYENYRDWIVNHNVAYAELFLDTAGVASFDVWDL
LPFTRGYVHILDKDPYLRHFAYDPQYFLNELDLLGQASALQLARNISNSG
AMQTYFAGETIPGDNLAYDADLMAWAEYLPYHFRPNYHGVGTCSMMSREM
GGVVDNAARVYGVQGLRVIDGSIPPTQVSSHVMTVFYAMALKIADAILED
YASMQHHHHHH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU5233
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression vector for expression of full length glucose oxidase with C-terminal 6His tag, under the control of the GAL10 promoter.