cDNA Clone glucose oxidase

• Plasmid: pRB1438 GST-glucose oxidase (23-605).6His
• Parent Plasmid: pRB1438
• Tag: GST
• Species: Aspergillus niger

Sequence of Insert

aagcttCTAACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATAGCAATGGCATTGAAGCAAACCTCCTGACCGATCCCAACG
ATGTCGCCGGCCGCACCGTCGACTACATCATCGCTGGTGGAGGTCTGACT
GGGCTCACCACCGCCGCCCGTCTGACTGAGGACCCCAACATCACCGTCCT
CGTCATTGAAAGTGGCTCCTACGAGTCAGACAGGGGTGCTATCATCGAGG
ACCTCAACGCCTACGGCGACATCTTTGGCAGCAGCGTCGACCATGCCTAC
GAGACCGTGACGCTCGCCACCAACAATCAGACTGCGCTGATCCGCTCCGG
AAATGGTCTCGGTGGCTCTACCTTGGTCAATGGTGGCACTTGGACTCGCC
CCCACAAAGCGCAAGTTGACTCATGGGAGACTGTCTTTGGCAATGAGGGC
TGGAACTGGGACAACGTGGCTGCCTACTCCCTCCAGGCGGAGCGTGCTCG
CGCACCCAACGCCAAACAGATCGCTGCTGGCCACTACTTCAACGCATCCT
GTCATGGTGTCAACGGCACTGTCCATGCCGGACCCCGCGACACCGGCGAT
GACTATTCTCCCATCGTCCAGGCTCTCATGAGCGCTGTGGAAGACAGGGG
CGTCCCTACCAAGAAGGACTTTGGATGCGGTGACCCCCATGGTGTGTCCA
TGTTCCCCAACACCTTGCACGAAGACCAAGTGCGCTCCGATGCCGCTCGC
GAATGGCTCCTTCCCAACTACCAGCGTCCCAACTTGCAAGTCCTGACCGG
ACAGTTTGTCGGAAAGGTCTTGCTCAACCAGAACACGACCACCCCTCGTG
CCGTCGGTGTGGAATTCGGCACGCACAAGGGCAACACCCACAACGTCTAC
GCGAAGCACGAGGTCCTCCTGGCCGCTGGCTCCGCTATCTCTCCCACCAT
CCTCGAATATTCCGGTATCGGCATGAAGTCCATCCTGGAGCCTCTTGGAA
TCGATACCGTCGTTGACCTTCCTGTCGGCCTGAACCTGCAGGACCAGACT
ACTGCTACCGTCCGCTCGCGCATCACCTCCGCCGGTGCCGGACAGGGACA
GGCTGCTTGGTTCGCCACCTTCAACGAGACCTTTGGTGACTACGCCGAAA
AGGCGCATGAGCTGCTGAACACCAAGCTGGAGCAGTGGGCCGAAGAGGCC
GTCGCCCGTGGCGGATTCCACAACACCACCGCCTTGCTCATCCAGTACGA
GAACTACCGCGACTGGATCGTCAACCACAATGTCGCGTACGCGGAACTTT
TCCTCGACACGGCCGGAGTGGCCAGCTTTGACGTCTGGGACCTCCTTCCC
TTCACGAGAGGATACGTCCATATTCTCGACAAGGACCCCTACCTCCGCCA
CTTCGCCTACGACCCTCAGTACTTCCTCAACGAGCTGGACCTGCTCGGTC
AGGCGTCCGCTCTTCAGCTGGCCCGGAACATCTCCAACTCGGGTGCCATG
CAGACGTACTTCGCAGGAGAGACCATTCCCGGTGATAACCTCGCGTATGA
TGCCGATTTGATGGCCTGGGCTGAGTATCTCCCGTACCACTTCCGTCCCA
ACTACCATGGAGTGGGAACGTGCTCCATGATGTCGAGGGAGATGGGCGGT
GTGGTCGACAATGCTGCCCGTGTGTATGGTGTGCAGGGGCTGCGGGTCAT
TGATGGTTCTATTCCGCCTACGCAGGTCTCGTCCCATGTGATGACGGTGT
TTTATGCCATGGCGCTGAAGATTGCGGACGCTATCTTGGAAGATTATGCT
TCCATGCAGCATCACCATCACCATCACTGAggtacc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDSNGIEANLLTDPNDVAGRTVDYIIAGGGLT
GLTTAARLTEDPNITVLVIESGSYESDRGAIIEDLNAYGDIFGSSVDHAY
ETVTLATNNQTALIRSGNGLGGSTLVNGGTWTRPHKAQVDSWETVFGNEG
WNWDNVAAYSLQAERARAPNAKQIAAGHYFNASCHGVNGTVHAGPRDTGD
DYSPIVQALMSAVEDRGVPTKKDFGCGDPHGVSMFPNTLHEDQVRSDAAR
EWLLPNYQRPNLQVLTGQFVGKVLLNQNTTTPRAVGVEFGTHKGNTHNVY
AKHEVLLAAGSAISPTILEYSGIGMKSILEPLGIDTVVDLPVGLNLQDQT
TATVRSRITSAGAGQGQAAWFATFNETFGDYAEKAHELLNTKLEQWAEEA
VARGGFHNTTALLIQYENYRDWIVNHNVAYAELFLDTAGVASFDVWDLLP
FTRGYVHILDKDPYLRHFAYDPQYFLNELDLLGQASALQLARNISNSGAM
QTYFAGETIPGDNLAYDADLMAWAEYLPYHFRPNYHGVGTCSMMSREMGG
VVDNAARVYGVQGLRVIDGSIPPTQVSSHVMTVFYAMALKIADAILEDYA
SMQHHHHHH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU5232
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression vector for expression of glucose oxidase residues 23-605 with N-terminal GST tag and C-terminal 6His tag, under the control of the GAL10 promoter.