cDNA Clone PIM1

• Plasmid: pFB1 GST-6His-thrombin-PIM1
• Parent Plasmid: pFastbac1
• Expressed: GST-6His-thrombin-PIM1
• Genbank: NM_002648
• Tag: GST
• Species: Human

Sequence of Insert

gaattcGAAACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCACCATCACCATCACCATCTGGTTCCGCGTGGAATGCTCT
TGTCCAAAATCAACTCGCTTGCCCACCTGCGCGCCGCGCCCTGCAACGAC
CTGCACGCCACCAAGCTGGCGCCCGGCAAGGAGAAGGAGCCCCTGGAGTC
GCAGTACCAGGTGGGCCCGCTACTGGGCAGCGGCGGCTTCGGCTCGGTCT
ACTCAGGCATCCGCGTCTCCGACAACTTGCCGGTGGCCATCAAACACGTG
GAGAAGGACCGGATTTCCGACTGGGGAGAGCTGCCTAATGGCACTCGAGT
GCCCATGGAAGTGGTCCTGCTGAAGAAGGTGAGCTCGGGTTTCTCCGGCG
TCATTAGGCTCCTGGACTGGTTCGAGAGGCCCGACAGTTTCGTCCTGATC
CTGGAGAGGCCCGAGCCGGTGCAAGATCTCTTCGACTTCATCACGGAAAG
GGGAGCCCTGCAAGAGGAGCTGGCCCGCAGCTTCTTCTGGCAGGTGCTGG
AGGCCGTGCGGCACTGCCACAACTGCGGGGTGCTCCACCGCGACATCAAG
GACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCATCGA
CTTCGGGTCGGGGGCGCTGCTCAAGGACACCGTCTACACGGACTTCGATG
GGACCCGAGTGTATAGCCCTCCAGAGTGGATCCGCTACCATCGCTACCAT
GGCAGGTCGGCGGCAGTCTGGTCCCTGGGGATCCTGCTGTATGATATGGT
GTGTGGAGATATTCCTTTCGAGCATGACGAAGAGATCATCAGGGGCCAGG
TTTTCTTCAGGCAGAGGGTCTCTTCAGAATGTCAGCATCTCATTAGATGG
TGCTTGGCCCTGAGACCATCAGATAGGCCAACCTTCGAAGAAATCCAGAA
CCATCCATGGATGCAAGATGTTCTCCTGCCCCAGGAAACTGCTGAGATCC
ACCTCCACAGCCTGTCGCCGGGGCCCAGCAAATAGactagt

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDHHHHHHLVPRGMLLSKINSLAHLRAAPCND
LHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHV
EKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLI
LERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIK
DENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYH
GRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRW
CLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5174
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid. Thrombin cleavage site between 6his tag and PIM1.