cDNA Clone PIM2

• Plasmid: pFB1 GST-6His-thrombin-PIM2
• Parent Plasmid: pFastbac1
• Expressed: GST-6His-thrombin-PIM2 (NM_006875.2 )
• Genbank: NM_006875
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGAAACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCACCATCACCATCACCATCTGGTTCCGCGTGGAATGTTGA
CCAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCG
CCAGGAGGCAAGGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCC
CCTCCTGGGTAAGGGGGGCTTTGGCACCGTCTTCGCAGGACACCGCCTCA
CAGATCGACTCCAGGTGGCCATCAAAGTGATTCCCCGGAATCGTGTGCTG
GGCTGGTCCCCCTTGTCAGACTCAGTCACATGCCCACTCGAAGTCGCACT
GCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGGCGTGATCCGCCTGC
TTGACTGGTTTGAGACACAGGAGGGCTTCATGCTGGTCCTCGAGCGGCCT
TTGCCCGCCCAGGATCTCTTTGACTATATCACAGAGAAGGGCCCACTGGG
TGAAGGCCCAAGCCGCTGCTTCTTTGGCCAAGTAGTGGCAGCCATCCAGC
ACTGCCATTCCCGTGGAGTTGTCCATCGTGACATCAAGGATGAGAACATC
CTGATAGACCTACGCCGTGGCTGTGCCAAACTCATTGATTTTGGTTCTGG
TGCCCTGCTTCATGATGAACCCTACACTGACTTTGATGGGACAAGGGTGT
ACAGCCCCCCAGAGTGGATCTCTCGACACCAGTACCATGCACTCCCGGCC
ACTGTCTGGTCACTGGGCATCCTCCTCTATGACATGGTGTGTGGGGACAT
TCCCTTTGAGAGGGACCAGGAGATTCTGGAAGCTGAGCTCCACTTCCCAG
CCCATGTCTCCCCAGACTGCTGTGCCCTAATCCGCCGGTGCCTGGCCCCC
AAACCTTCTTCCCGACCCTCACTGGAAGAGATCCTGCTGGACCCCTGGAT
GCAAACACCAGCCGAGGATGTACCCCTCAACCCCTCCAAAGGAGGCCCTG
CCCCTTTGGCCTGGTCCTTGCTACCCTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDHHHHHHLVPRGMLTKPLQGPPAPPGTPTPP
PGGKDREAFEAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVL
GWSPLSDSVTCPLEVALLWKVGAGGGHPGVIRLLDWFETQEGFMLVLERP
LPAQDLFDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENI
LIDLRRGCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPA
TVWSLGILLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAP
KPSSRPSLEEILLDPWMQTPAEDVPLNPSKGGPAPLAWSLLP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5173
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid. Thrombin cleavage site between 6His tag and PIM2.