cDNA Clone PIM2

• Plasmid: pFBHTb PIM2 (atg orf)
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PIM2 (atg orf) (NM_006875.2 )
• Genbank: NM_006875.2 GI:42821111
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccTTGACCAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAAGGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAGGGGGGCTTTGGCACCGTCTTCGCAGGACAC
CGCCTCACAGATCGACTCCAGGTGGCCATCAAAGTGATTCCCCGGAATCG
TGTGCTGGGCTGGTCCCCCTTGTCAGACTCAGTCACATGCCCACTCGAAG
TCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGGCGTGATC
CGCCTGCTTGACTGGTTTGAGACACAGGAGGGCTTCATGCTGGTCCTCGA
GCGGCCTTTGCCCGCCCAGGATCTCTTTGACTATATCACAGAGAAGGGCC
CACTGGGTGAAGGCCCAAGCCGCTGCTTCTTTGGCCAAGTAGTGGCAGCC
ATCCAGCACTGCCATTCCCGTGGAGTTGTCCATCGTGACATCAAGGATGA
GAACATCCTGATAGACCTACGCCGTGGCTGTGCCAAACTCATTGATTTTG
GTTCTGGTGCCCTGCTTCATGATGAACCCTACACTGACTTTGATGGGACA
AGGGTGTACAGCCCCCCAGAGTGGATCTCTCGACACCAGTACCATGCACT
CCCGGCCACTGTCTGGTCACTGGGCATCCTCCTCTATGACATGGTGTGTG
GGGACATTCCCTTTGAGAGGGACCAGGAGATTCTGGAAGCTGAGCTCCAC
TTCCCAGCCCATGTCTCCCCAGACTGCTGTGCCCTAATCCGCCGGTGCCT
GGCCCCCAAACCTTCTTCCCGACCCTCACTGGAAGAGATCCTGCTGGACC
CCTGGATGCAAACACCAGCCGAGGATGTACCCCTCAACCCCTCCAAAGGA
GGCCCTGCCCCTTTGGCCTGGTCCTTGCTACCCTAAggatcc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSLTKPLQGPPAPPGTPTPPPGGK
DREAFEAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVLGWSP
LSDSVTCPLEVALLWKVGAGGGHPGVIRLLDWFETQEGFMLVLERPLPAQ
DLFDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENILIDL
RRGCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWS
LGILLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPSS
RPSLEEILLDPWMQTPAEDVPLNPSKGGPAPLAWSLLP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5143
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 37406 Daltons