cDNA Clone EVL-1

• Plasmid: pGEX6P-3-EVL-1
• Parent Plasmid: pGEX6P3
• Expressed: GST-EVL-1
• Genbank: BC023997
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGAGTGAACAGAGTATCTGCCAAGCCCGGGCTTCCGTGATGGTCTACGATGACACCAGTAAGAAATGGGTACCAATCAAACCTGGCCAGCAGGGATTCAGCCGGATCAACATCTACCACAACACTGCCAGCAACACCTTCAGAGTCGTTGGAGTCAAGTTGCAGGATCAGCAGGTTGTGATCAATTATTCAATCGTGAAAGGGCTGAAGTACAATCAGGCCACGCCAACCTTCCACCAGTGGCGAGATGCCCGCCAGGTCTACGGCTTAAACTTTGCAAGTAAAGAAGAGGCAACCACG
TTCTCCAATGCAATGCTGTTTGCCCTGAACATCATGAATTCCCAAGAAGG
AGGCCCCTCCAGCCAGCGTCAGGTGCAGAATGGCCCCTCTCCTGATGAGA
TGGACATCCAGAGAAGACAAGTGATGGAGCAGCACCAGCAGCAGCGTCAG
GAATCTCTAGAAAGAAGAACCTCGGCCACAGGGCCCATCCTCCCACCAGG
ACATCCTTCATCTGCAGCCAGCGCCCCCGTCTCATGTAGTGGGCCTCCAC
CGCCCCCCCCACCCCCAGTCCCACCTCCACCCACTGGGGCTACCCCACCT
CCCCCACCCCCACTGCCAGCCGGAGGAGCCCAGGGGTCCAGCCACGACGA
GAGCTCCATGTCAGGACTGGCCGCTGCCATAGCTGGGGCCAAGCTGAGAA
GAGTCCAACGGCCAGAAGACGCATCTGGAGGCTCCAGTCCCAGTGGGACC
TCAAAGTCCGATGCCAACCGGGCAAGCAGCGGGGGTGGCGGAGGAGGCCT
CATGGAGGAAATGAACAAACTGCTGGCCAAGAGGAGAAAAGCAGCCTCCC
AGTCAGACAAGCCAGCCGAGAAGAAGGAAGATGAAAGCCAAATGGAAGAT
CCTAGTACCTCCCCCTCTCCGGGGACCCGAGCAGCCAGCCAGCCACCTAA
CTCCTCAGAGGCTGGCCGGAAGCCCTGGGAGCGGAGCAACTCGGTGGAGA
AGCCTGTGTCCTCGATTCTGTCCAGAACCCCGTCTGTGGCAAAGAGCCCC
GAAGCTAAGAGCCCCCTTCAGTCGCAGCCTCACTCTAGGATGAAGCCTGC
TGGGAGCGTGAATGACATGGCCCTGGATGCCTTCGACTTGGACCGGATGA
AGCAGGAGATCCTAGAGGAGGTGGTGAGAGAGCTCCACAAGGTGAAGGAG
GAGATCATCGACGCCATCAGGCAGGAGCTGAGTGGGATCAGCACCACGTA
Agcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSEQSICQARASVMVYDDT
SKKWVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQVVINYSIVKGL
KYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGP
SSQRQVQNGPSPDEMDIQRRQVMEQHQQQRQESLERRTSATGPILPPGHP
SSAASAPVSCSGPPPPPPPPVPPPPTGATPPPPPPLPAGGAQGSSHDESS
MSGLAAAIAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLME
EMNKLLAKRRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNSS
EAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGS
VNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5123
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for bacterial expression of GST N-terminal fusion (with PreScission Protease cleavage site).