cDNA Clone RINT1

• Plasmid: pCMV5-FLAG RINT1
• Parent Plasmid: pCMV5
• Expressed: FLAG-RINT1
• Genbank: NM_021930
• Tag: FLAG
• Species: Human

Sequence of Insert

ggtaccGCCACCATGGACTACAAGGACGACGATGACAAGGGTAGTGAGTGTGTCGCTGGCCTTAGCCAGACTCCACAGGCCACGCTGGCTGCGAATGGAGCCGAGGACTCGCGCGGAGGCGAGATGCTACCAGCCGGCGAGATCGGCGCCTCTCCTGCAGCCCCGTGCTGCTCTGAAAGTGGCGACGAAAGGAAGAACCTCGAGGAGAAAAGTGACATAAATGTTACAGTTCTTATTGGATGTAAACAAGTCAGTGAAGGTACAGATAATGGTGATCTCCCTTCTTATGTGTCTGCATTCATAGAAAAGGAAGTTGGAAATGACCTTAAATCTTTAAAGAAACTTGATAAACTCATAGAACAGAGGACAGTAAGTAAAATGCAGTTAGAAGAACAGGTACTTACAATTTCATCAGAAATTCCTAAAAGAATTCGAAGTGCCTTAAAAAATGCAGAAGAATCAAAGCAATTTCTTAATCAGTTTCTGGAGCAGGAAACTCATCTCTTCAGCGCCATTAACAGCCATTTGCTGACTGCGCAACCTTGGATGGACGATCTTGGAACCATGATTAGCCAGATTGAAGAGATCGAACGTCATCTTGCTTACCTTAAATGGATTTCACAAATTGAAGAACTAAGTGATAACATTCAGCAATATCTGATGACCAATAATGTACCGGAGGCAGCCTCCACTCTAGTGTCTATGGCAGAACTTGACATTAAACTTCAGGAATCATCTTGTACTCATCTTCTTGGTTTCATGAGAGCCACAGTTAAATTCTGGCATAAAATTCTCAAGGACAAGCTTACAAGTGATTTTGAGGAAATTTTAGCACAGCTTCATTGGCCATTCATCGCACCCCCTCAATCACAAACTGTTGGCTTAAGTCGACCTGCCAGTGCCCCGGAGATATACAGTTACCTGGAGACACTGTTTTGTCAGCTTTTGAAACTACAAACCTCAGATGAATTACTTACTGAGCCAAAGCAACTCCCAGAAAAATACTCTCTTCCTGCCTCCCCTTCTGTCATCCTGCCCATCCAGGTTATGCTGACTCCTCTTCAGAAGAGGTTCAGGTATCACTTCAGAGGGAACCGGCAGACTAATGTGTTAAGCAAGCCAGAATGGTACTTGGCTCAAGTACTTATGTGGATTGGAAACCATACTGAATTTCTGGATGAGAAGATTCAGCCAATATTAGACAAAGTAGGCTCTTTGGTAAACGCAAGGCTTGAATTTTCTCGGGGCCTTATGATGCTGGTTCTTGAGAAGTTAGCCACTGATATTCCTTGTCTGCTATATGATGACAATCTCTTCTGTCATTTGGTGGATGAAGTACTCTTGTTTGAAAGGGAGCTACACAGTGTTCATGGCTATCCTGGCACTTTTGCTAGTTGTATGCATATTCTATCAGAGGAAACCTGTTTTCAGAGATGGTTGACGGTGGAGAGAAAATTTGCTCTTCAAAAAATGGACTCAATGCTTTCCTCAGAAGCCGCCTGGGTATCGCAATATAAGGATATCACTGACGTGGATGAAATGAAAGTTCCAGATTGTGCAGAAACTTTTATGACTCTACTCCTGGTTATAACTGACAGGTATAAAAATCTTCCCACAGCTTCCCGAAAGCTTCAGTTCCTGGAGTTACAGAAGGACTTAGTAGATGATTTTAGGATACGATTAACACAAGTGATGAAAGAAGAGACTAGAGCTTCCCTTGGCTTTCGATACTGTGCAATTCTTAATGCTGTGAACTACATCTCAACAGTACTAGCAGATTGGGCTGACAATGTTTTCTTTCTACAACTTCAACAGGCTGCACTGGAGGTGTTTGCA
GAGAATAATACTCTGAGTAAATTGCAGCTAGGACAGCTAGCCTCTATGGA
GAGCTCTGTCTTTGATGACATGATTAACCTCTTAGAACGTTTAAAGCATG
ATATGTTGACCCGTCAAGTAGACCACGTTTTTAGAGAAGTTAAAGATGCT
GCAAAATTGTATAAAAAAGAAAGATGGTTGTCCTTGCCATCTCAGTCAGA
GCAGGCCGTGATGTCCCTGTCCAGTTCGGCTTGCCCGTTGCTGCTGACGT
TACGAGACCATTTACTTCAGTTGGAGCAGCAGCTTTGTTTCTCCTTATTT
AAAATTTTCTGGCAAATGCTTGTAGAGAAGCTGGATGTATACATCTACCA
AGAAATAATTCTTGCTAATCACTTCAATGAAGGGGGAGCAGCCCAGCTGC
AGTTTGATATGACTCGGAATCTTTTCCCTTTGTTTTCTCACTATTGCAAG
AGACCAGAAAATTATTTTAAACATATAAAAGAAGCCTGTATTGTTTTGAA
TTTGAACGTCGGTTCTGCACTACTGCTGAAAGATGTACTGCAGTCAGCTT
CAGGGCAGCTTCCTGCCACAGCAGCATTAAATGAAGTTGGAATTTACAAA
CTGGCTCAACAAGATGTTGAGATTCTACTTAATTTGAGGACAAATTGGCC
TAATACTGGAAAATAAggatcc

Amino Acid Sequence

MDYKDDDDKGSECVAGLSQTPQATLAANGAEDSRGGEMLPAGEIGASPAA
PCCSESGDERKNLEEKSDINVTVLIGCKQVSEGTDNGDLPSYVSAFIEKE
VGNDLKSLKKLDKLIEQRTVSKMQLEEQVLTISSEIPKRIRSALKNAEES
KQFLNQFLEQETHLFSAINSHLLTAQPWMDDLGTMISQIEEIERHLAYLK
WISQIEELSDNIQQYLMTNNVPEAASTLVSMAELDIKLQESSCTHLLGFM
RATVKFWHKILKDKLTSDFEEILAQLHWPFIAPPQSQTVGLSRPASAPEI
YSYLETLFCQLLKLQTSDELLTEPKQLPEKYSLPASPSVILPIQVMLTPL
QKRFRYHFRGNRQTNVLSKPEWYLAQVLMWIGNHTEFLDEKIQPILDKVG
SLVNARLEFSRGLMMLVLEKLATDIPCLLYDDNLFCHLVDEVLLFERELH
SVHGYPGTFASCMHILSEETCFQRWLTVERKFALQKMDSMLSSEAAWVSQ
YKDITDVDEMKVPDCAETFMTLLLVITDRYKNLPTASRKLQFLELQKDLV
DDFRIRLTQVMKEETRASLGFRYCAILNAVNYISTVLADWADNVFFLQLQ
QAALEVFAENNTLSKLQLGQLASMESSVFDDMINLLERLKHDMLTRQVDH
VFREVKDAAKLYKKERWLSLPSQSEQAVMSLSSSACPLLLTLRDHLLQLE
QQLCFSLFKIFWQMLVEKLDVYIYQEIILANHFNEGGAAQLQFDMTRNLF
PLFSHYCKRPENYFKHIKEACIVLNLNVGSALLLKDVLQSASGQLPATAA
LNEVGIYKLAQQDVEILLNLRTNWPNTGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5088
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. sequence changes compared with NM_021930:; t147c (silent in G49), a205t (S69C), t1461c (silent in A87), t1546c (silent in 516L), a2007c (silent in A669), a2184g (silent in G728), a2365g (T789A),t2362c (S788P). ; All changes present in all M. Peggie RINT1 constructs; Residue changes present in ESTs for RINT1. S788P and T789A present in several other mRNA sequences for RINT1.; Estimated MW of expressed protein : 94372 Daltons