cDNA Clone MKK2 (MEK2)

• Synonyms: MAP2K2
• Plasmid: pGEX4T-1-MKK2 6His
• Parent Plasmid: pGEX4T1
• Expressed: GST-MKK2 6His
• Genbank: P36507
• Tag: GST
• Species: Human

Sequence of Insert

ggatccCTGGCCCGGAGGAAGCCGGTGCTGCCGGCGCTCACCATCAACCC
TACCATCGCCGAGGGCCCATCCCCTACCAGCGAGGGCGCCTCCGAGGCAA
ACCTGGTGGACCTGCAGAAGAAGCTGGAGGAGCTGGAACTTGACGAGCAG
CAGAAGAAGCGGCTGGAAGCCTTTCTCACCCAGAAAGCCAAGGTTGGCGA
ACTCAAAGACGATGACTTCGAAAGGATCTCAGAGCTGGGCGCGGGCAACG
GCGGGGTGGTCACCAAAGTCCAGCACAGACCCTCGGGCCTCATCATGGCC
AGGAAGCTGATCCACCTTGAGATCAAGCCGGCCATCCGGAACCAGATCAT
CCGCGAGCTGCAGGTCCTGCACGAATGCAACTCGCCGTACATCGTGGGCT
TCTACGGGGCCTTCTACAGTGACGGGGAGATCAGCATTTGCATGGAACAC
ATGGACGGCGGCTCCCTGGACCAGGTGCTGAAAGAGGCCAAGAGGATTCC
CGAGGAGATCCTGGGGAAAGTCAGCATCGCGGTTCTCCGGGGCTTGGCGT
ACCTCCGAGAGAAGCACCAGATCATGCACCGAGATGTGAAGCCCTCCAAC
ATCCTCGTGAACTCTAGAGGGGAGATCAAGCTGTGTGACTTCGGGGTGAG
CGGCCAGCTCATAGACTCCATGGCCAACTCCTTCGTGGGCACGCGCTCCT
ACATGGCTCCGGAGCGGTTGCAGGGCACACATTACTCGGTGCAGTCGGAC
ATCTGGAGCATGGGCCTGTCCCTGGTGGAGCTGGCCGTCGGAAGGTACCC
CATCCCCCCGCCCGACGCCAAAGAGCTGGAGGCCATCTTTGGCCGGCCCG
TGGTCGACGGGGAAGAAGGAGAGCCTCACAGCATCTCGCCTCGGCCGAGG
CCCCCCGGGCGCCCCGTCAGCGGTCACGGGATGGATAGCCGGCCTGCCAT
GGCCATCTTTGAACTCCTGGACTATATTGTGAACGAGCCACCTCCTAAGC
TGCCCAACGGTGTGTTCACCCCCGACTTCCAGGAGTTTGTCAATAAATGC
CTCATCAAGAACCCAGCGGAGCGGGCGGACCTGAAGATGCTCACAAACCA
CACCTTCATCAAGCGGTCCGAGGTGGAAGAAGTGGATTTTGCCGGCTGGT
TGTGTAAAACCCTGCGGCTGAACCAGCCCGGCACACCCACGCGCACCGCC
GTGCACCATCACCATCACCATTAAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLARRKPVLPALTINPTIAEGPSPT
SEGASEANLVDLQKKLEELELDEQQKKRLEAFLTQKAKVGELKDDDFERI
SELGAGNGGVVTKVQHRPSGLIMARKLIHLEIKPAIRNQIIRELQVLHEC
NSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEAKRIPEEILGKVSI
AVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMAN
SFVGTRSYMAPERLQGTHYSVQSDIWSMGLSLVELAVGRYPIPPPDAKEL
EAIFGRPVVDGEEGEPHSISPRPRPPGRPVSGHGMDSRPAMAIFELLDYI
VNEPPPKLPNGVFTPDFQEFVNKCLIKNPAERADLKMLTNHTFIKRSEVE
EVDFAGWLCKTLRLNQPGTPTRTAVHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5085
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion with thrombin cleavage site.; Estimated MW of expressed protein : 71362 Daltons