cDNA Clone PIM2

• Plasmid: pGEX4T-3-PIM2 new
• Parent Plasmid: pGEX4T3
• Expressed: GST-PIM2 (NM_006875.2 )
• Genbank: NM_006875.2 GI:42821111 (N.B. This record replaced NM_006875.1 GI:5803124 on 26-FEB-2004)
• Tag: GST
• Species: Human

Sequence of Insert

ggatccTTGACCAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAAGGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAGGGGGGCTTTGGCACCGTCTTCGCAGGACAC
CGCCTCACAGATCGACTCCAGGTGGCCATCAAAGTGATTCCCCGGAATCG
TGTGCTGGGCTGGTCCCCCTTGTCAGACTCAGTCACATGCCCACTCGAAG
TCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGGCGTGATC
CGCCTGCTTGACTGGTTTGAGACACAGGAGGGCTTCATGCTGGTCCTCGA
GCGGCCTTTGCCCGCCCAGGATCTCTTTGACTATATCACAGAGAAGGGCC
CACTGGGTGAAGGCCCAAGCCGCTGCTTCTTTGGCCAAGTAGTGGCAGCC
ATCCAGCACTGCCATTCCCGTGGAGTTGTCCATCGTGACATCAAGGATGA
GAACATCCTGATAGACCTACGCCGTGGCTGTGCCAAACTCATTGATTTTG
GTTCTGGTGCCCTGCTTCATGATGAACCCTACACTGACTTTGATGGGACA
AGGGTGTACAGCCCCCCAGAGTGGATCTCTCGACACCAGTACCATGCACT
CCCGGCCACTGTCTGGTCACTGGGCATCCTCCTCTATGACATGGTGTGTG
GGGACATTCCCTTTGAGAGGGACCAGGAGATTCTGGAAGCTGAGCTCCAC
TTCCCAGCCCATGTCTCCCCAGACTGCTGTGCCCTAATCCGCCGGTGCCT
GGCCCCCAAACCTTCTTCCCGACCCTCACTGGAAGAGATCCTGCTGGACC
CCTGGATGCAAACACCAGCCGAGGATGTACCCCTCAACCCCTCCAAAGGA
GGCCCTGCCCCTTTGGCCTGGTCCTTGCTACCCTAAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLTKPLQGPPAPPGTPTPPPGGKDR
EAFEAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVLGWSPLS
DSVTCPLEVALLWKVGAGGGHPGVIRLLDWFETQEGFMLVLERPLPAQDL
FDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENILIDLRR
GCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLG
ILLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPSSRP
SLEEILLDPWMQTPAEDVPLNPSKGGPAPLAWSLLP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5065
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 60312 Daltons