cDNA Clone PPP5C

• Plasmid: pGEX6P-1-PPP5C 1-425
• Parent Plasmid: pGEX6P1
• Expressed: GST-PPP5C 1-425
• Genbank: NM_006247
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCGATGGCGGAGGGCGAGAGGACTGAGTGTGCTGAGCCCCCCCGGGACGAACCCCCGGCTGATGGAGCTCTGAAGCGGGCAGAGGAGCTCAAGACTCAGGCCAATGACTACTTCAAAGCCAAGGACTACGAGAACGCCATCAAGTTCTACAGCCAGGCCATCGAGCTGAACCCCAGCAATGCCATCTACTATGGCAACCGCAGCCTGGCCTACCTGCGCACTGAGTGCTATGGCTACGCGCTGGGAGACGCCACGCGGGCCATTGAGCTGGACAAGAAGTACATCAAGGGTTATTAC
CGCCGGGCTGCCAGCAACATGGCACTGGGCAAGTTCCGGGCCGCGCTGCGAGACTACGAGACGGTGGTCAAGGTGAAGCCCCATGACAAGGATGCCAAAATGAAATACCAGGAGTGCAACAAGATCGTGAAGCAGAAGGCCTTTGAGCGGGCCATCGCGGGCGACGAGCACAAGCGCTCCGTGGTGGACTCGCTGGACATCGAGAGCATGACCATTGAGGATGAGTACAGCGGACCCAAGCTTGAAGACGGCAAAGTGACAATCAGTTTCATGAAGGAGCTCATGCAGTGGTACAAGGACCAGAAGAAACTGCACCGGAAATGTGCCTACCAGATTCTGGTACAGGTCAAAGAGGTCCTCTCCAAGCTGAGCACGCTCGTGGAAACCACACTCAAAGAGACAGAGAAGATTACAGTATGTGGGGACACCCATGGCCAGTTCTATGACCTCCTCAACATATTCGAGCTCAACGGTTTACCCTCGGAGACCAACCCCTATATATTTAATGGTGACTTTGTGGACCGAGGCTCCTTCTCTGTAGAAGTGATCCTCACCCTTTTCGGCTTCAAGCTCCTGTACCCAGATCACTTTCACCTCCTTCGAGGCAACCACGAGACAGACAACATGAACCAGATCTACGGTTTCGAGGGTGAGGTGAAGGCCAAGTACACAGCCCAGATGTACGAGCTCTTTAGCGAGGTGTTCGAGTGGCTCCCGTTGGCCCAGTGCATCAACGGCAAAGTGCTGATCATGCACGGAGGCCTGTTCAGTGAAGACGGTGTCACCCTGGATGACATCCGGAAAATTGAGCGGAATCGACAACCCCCAGATTCAGGGCCCATGTGTGACCTGCTCTGGTCAGATCCACAGCCACAGAACGGGCGCTCGATCAGCAAGCGGGGCGTGAGCTGTCAGTTTGGGCCTGACGTCACCAAGGCCTTCTTGGAAGAGAACAACCTGGACTATATCATCCGCTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAMAEGERTECAEPPRDEP
PADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRS
LAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDY
ETVVKVKPHDKDAKMKYQECNKIVKQKAFERAIAGDEHKRSVVDSLDIES
MTIEDEYSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAYQILVQVKEV
LSKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFN
GDFVDRGSFSVEVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEV
KAKYTAQMYELFSEVFEWLPLAQCINGKVLIMHGGLFSEDGVTLDDIRKI
ERNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENN
LDYIIR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1481
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Estimated MW of expressed protein : 75480