cDNA Clone Pdpc1

• Plasmid: pGEX6P-1-Pdpc1
• Parent Plasmid: pGEX6P1
• Expressed: GST-Pdpc1
• Genbank: NM_018444
• Tag: GST
• Species: Human

Sequence of Insert

ggatccTTGTCGGCTCCGTGTTGTGATGACAGGAGAATGTGTGTGTGTCCCGGGCCCAGACGAATTGGAATCCCAGTCAGAAGTTCCAGCCTGCCACTGTTCTCTGATGCCATGCCAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAAC
TGTGAACTGAGCAGGATCTATGGCACTGCATGTTACTGCCACCACAAACA
TCTCTGTTGTTCCTCATCGTACATTCCTCAGAGTCGACTGAGATACACAC
CTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAGAACTGGTGGCAG
TACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTCAC
ACCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAG
TGCCAGAATTTGACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGC
AATCAGCTGCCTGCAAATGCACCCATTGAGGACCGGAGAAGTGCAGCAAC
CTGCTTGCAGACCAGAGGGATGCTTTTGGGGGTTTTTGATGGCCATGCAG
GTTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTTTATTATATTGCT
GTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGA
GAGCGGCCGGGCACTGCTACCCATTCTCCAGTGGCACAAGCACCCCAATG
ATTACTTTAGTAAGGAGGCATCCAAATTGTACTTTAACAGCTTGAGGACT
TACTGGCAAGAGCTTATAGACCTCAACACTGGTGAGTCGACTGATATTGA
TGTTAAGGAGGCTCTAATTAATGCCTTCAAGAGGCTTGATAATGACATCT
CCTTGGAGGCGCAAGTTGGTGATCCTAATTCTTTTCTCAACTACCTGGTG
CTTCGAGTGGCATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGATGG
TGTTGACCTTCATGTGGCCAATACTGGCGATAGCAGAGCCATGCTGGGTG
TGCAGGAAGAGGACGGCTCATGGTCAGCAGTCACGCTGTCTAATGACCAC
AATGCTCAAAATGAAAGAGAACTAGAACGGCTGAAATTGGAACATCCAAA
GAGTGAGGCCAAGAGTGTCGTGAAACAGGATCGGCTGCTTGGCTTGCTGA
TGCCATTTAGGGCATTTGGAGATGTAAAGTTCAAATGGAGCATTGACCTT
CAAAAGAGAGTGATAGAATCTGGCCCAGACCAGTTGAATGACAATGAATA
TACCAAGTTTATTCCTCCTAATTATCACACACCTCCTTATCTCACTGCTG
AGCCAGAGGTAACTTACCACCGATTAAGGCCACAGGATAAGTTTCTGGTG
TTGGCTACTGATGGGTTGTGGGAGACTATGCATAGGCAGGATGTGGTTAG
GATTGTGGGTGAGTACCTAACTGGCATGCATCACCAACAGCCAATAGCTG
TTGGTGGCTACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAA
AGGAGAACCAAAATGTCCTCGGTATTTGAGGATCAGAACGCAGCAACCCA
TCTCATTCGCCACGCTGTGGGCAACAACGAGTTTGGGACTGTTGATCATG
AGCGCCTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGTAC
AGAGATGACATTACAATCATTGTAGTTCAGTTCAATTCTCATGTTGTAGG
GGCGTATCAAAACCAAGAAAAGTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLSAPCCDDRRMCVCPGPRRIGIPVRSSSLPLFSDAMPAPTQLFFPLIRNCELSRIYGTACYCHHKHLCCSSSYIPQSRLRYTPHPAYATFCRPKENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESTDIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKSEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRTKMSSVFEDQNAATHLIRHAVGNNEFGTVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQEK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1375
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express Pdpc1 with N-terminal GST.; ; N.B. sequence changes compared with NM_018444 CDS: ; Single nucleotide insertion after nt 421 (causing frame shift).; Single nucleotide deletion at a454 (frame reverts back to original).; Point mutations g561t (W187C), g602t (G201V), g610t (V204L). ; All changes present in all available EST sequences, therefore assumed to be correct.