cDNA Clone PTIP

• Plasmid: pFastBac.HTa hPTIP
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PTIP
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcTCGGACCAGGCGCCCAAAGTTCCTGAGGAGATGTTCAGGGAGGTCAAGTATTACGCGGTGGGCGACATCGACCCGCAGGTTATTCAGCTTCTCAAGGCTGGAAAAGCGAAGGAAGTTTCCTACAATGCACTAGCCTCACACATAATC
TCAGAGGATGGGGACAATCCAGAGGTGGGAGAAGCTCGGGAAGTCTTTGA
CTTACCTGTTGTAAAGCCTTCTTGGGTGATTCTGTCCGTTCAGTGTGGAA
CTCTTCTGCCAGTAAATGGTTTTTCTCCAGAATCATGTCAGATTTTTTTT
GGAATCACTGCCTGCCTTTCTCAGGTGTCATCTGAAGACAGAAGTGCCCT
GTGGGCTTTGGTTACGTTCTATGGGGGAGATTGCCAGCTAACCCTCAATA
AGAAATGCACGCATTTGATTGTTCCAGAGCCAAAGGGGGAGAAATACGAA
TGTGCTTTAAAGCGAGCAAGTATTAAAATTGTGACTCCTGACTGGGTTCT
GGATTGCGTATCAGAGAAAACCAAAAAGGACGAAGCATTTTATCATCCTC
GTCTGATTATTTATGAAGAGGAAGAAGAGGAAGAGGAAGAGGAGGAGGAA
GTAGAAAATGAGGAACAAGATTCTCAGAATGAGGGTAGTACAGATGAGAA
GTCAAGCCCTGCCAGCTCTCAAGAAGGGTCTCCTTCAGGTGACCAGCAGT
TTTCACCTAAATCCAACACTGAAAAATCTAAAGGGGAATTAATGTTTGAT
GATTCTTCAGATTCATCACCGGAAAAACAGGAGAGAAATTTAAACTGGAC
CCCGGCCGAAGTCCCACAGTTAGCTGCAGCAAAACGCAGGCTGCCTCAGG
GAAAGGAGCCTGGGTTGATTAACTTGTGTGCCAATGTCCCACCCGTCCCA
GGTAACATTTTGCCCCCTGAGGTCCGGGGTAATTTAATGGCTGCTGGACA
AAACCTCCAAAGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGTCCAG
CTGTACGGACACTGAGGAATATTACTAATAATGCTGACATTCAGCAGATG
AACCGGCCATCAAATGTAGCACATATCCTACAGACTCTCTCAGCACCTAC
GAAAAATTTAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACAAATG
CCAATGCAGTGCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACACATG
CTACAGCAGCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCGGTTTT
ACACCTTCAGCCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCAGCAGC
AGATCTCTCAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTTCACAG
CAACAGCAGCAGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAGCAACA
GCTACAGTTTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCATCGCC
CTCAGCAGCAGCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGCAGCAG
TTCCATCAGCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTCGCCCAGCT
CCAGCAGCAGCACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGATTCAGC
AGCAGCAGCTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGATGCAA
AGTCAGACAGCGCCACACTTGAGTCAGACGTCACAGGCGCTGCAGCATCA
GGTTCCACCTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCCACCAC
CATCGCCTCAGCAGCATCAGCTTTTTGGACATGATCCAGCAGTGGAGATT
CCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCC
AGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCC
AGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCAC
CTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGA
AAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAGA
AGAAAATGGTACCGCCGCACCGAGCCCTTCACTTCCCAGTGGCCTTCCCA
CCAGGAGGAAAGCCATGTTCACAGCATATTATTTCTGTGACTGGATTTGT
TGATAGTGACAGAGATGACCTAAAATTAATGGCTTATTTGGCAGGTGCCA
AATATACGGGTTATCTATGCCGCAGCAACACAGTCCTCATCTGTAAAGAA
CCAACTGGTTTAAAGTATGAAAAAGCCAAAGAGTGGAGGATACCCTGTGT
CAACGCCCAGTGGCTTGGCGACATTCTTCTGGGAAACTTTGAGGCACTGA
GGCAGATTCAGTATAGTCGCTACACGGCATTCAGTCTGCAGGATCCATTT
GCCCCTACCCAGCATTTAGTTTTAAATCTTTTAGATGCTTGGAGAGTTCC
CTTAAAAGTGTCTGCAGAGTTGTTGATGAGTATAAGACTACCTCCCAAAC
TGAAACAGAATGAAGTAGCTAATGTCCAGCCTTCTTCCAAAAGAGCCAGA
ATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGACCCC
TTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATATTA
AGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGC
ACACACCTCATTGCCAGCAAGGTGACTCGCACCGTGAAGTTCCTGACGGC
GATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAATGCT
TCAGGTGTCAGAAGTTCATTGATGAGCAGAACTACATTCTCCGAGATGCT
GAGGCAGAAGTACTTTTCTCTTTCAGCTTGGAAGAATCCTTAAAACGGGC
ACACGTTTCTCCACTCTTTAAGGCAAAATATTTTTACATCACACCTGGAA
TCTGCCCAAGTCTTTCCACTATGAAGGCAATCGTAGAGTGTGCAGGAGGA
AAGGTGTTATCCAAGCAGCCATCTTTCCGGAAGCTCATGGAGCACAAGCA
GAACTCGAGTTTGTCGGAAATAATTTTAATATCCTGTGAAAATGACCTTC
ATTTATGCCGAGAATATTTTGCCAGAGGCATAGATGTTCACAATGCAGAG
TTCGTTCTGACTGGAGTGCTCACTCAAACGCTGGACTATGAATCATATAA
GTTTAACTGAactagt

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFSDQAPKVPEEMFREVKYYAVGDIDPQVIQLLKAGKAKEVSYNALASHIISEDGDNPEVGEAREVFDLPVVKPSWVILSVQCGTLLPVNGFSPESCQIFFGITACLSQVSSEDRSALWALVTFYGGDCQLTLNKKCTHLIVPEPKGEKYECALKRASIKIVTPDWVLDCVSEKTKKDEAFYHPRLIIYEEEEEEEEEEEEVENEEQDSQNEGSTDEKSSPASSQEGSPSGDQQFSPKSNTEKSKGELMFDDSSDSSPEKQERNLNWTPAEVPQLAAAKRRLPQGKEPGLINLCANVPPVPGNILPPEVRGNLMAAGQNLQSSERSEMIATWSPAVRTLRNITNNADIQQMNRPSNVAHILQTLSAPTKNLEQQVNHSQQGHTNANAVLFSQVKVTPETHMLQQQQQAQQQQQQHPVLHLQPQQIMQLQQQQQQQISQQPYPQQPPHPFSQQQQQQQQAHPHQFSQQQLQFPQQQLHPPQQLHRPQQQLQPFQQQHALQQQFHQLQQHQLQQQQLAQLQQQHSLLQQQQQQQIQQQQLQRMHQQQQQQQMQSQTAPHLSQTSQALQHQVPPQQPPQQQQQQQPPPSPQQHQLFGHDPAVEIPEEGFLLGCVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCESQVSSAYAQAIRERKRCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGGKPCSQHIISVTGFVDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPTQHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLTPELTPFVLFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHLIASKVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAEVLFSFSLEESLKRAHVSPLFKAKYFYITPGICPSLSTMKAIVECAGGKVLSKQPSFRKLMEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLTQTLDYESYKFN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1307
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag