cDNA Clone PACS2

• Plasmid: pEBG6P-PACS2 S487A
• Parent Plasmid: pEBG6P
• Expressed: GST-PACS2 S487A
• Genbank: XP_290619. [gi:37546025]
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGTGTCAGGCGGCCCATCCGTCTGACTGCAACTGCACGCTCGC
CAGCCATGTGACCGCGCCGCCGCCCTCCGCGCGCCCGGCCCGCCCGCCGC
GCGTCCGCGGCCCGGCCGCAGCCCCAGGCCGCCGAGGGAGCGGCGGGGCC
GGCGCCATGGCCGAGCGAGGCCGCCTCGGCCTCCCCGGCGCGCCCGGCGC
GCTCAACACGCCCGTGCCCATGAACCTGTTCGCCACCTGGGAGGTGGACG
GCTCCAGCCCCAGCTGCGTGCCCAGGTTGTGCAGCCTGACTCTGAAGAAG
CTGGTGGTCTTCAAGGAGCTGGAGAAGGAGCTGATCTCCGTGGTGATCGC
TGTCAAGATGCAGGGCTCCAAACGAATCCTGCGGTCCCATGAGATTGTGC
TGCCCCCCAGTGGACAAGTGGAGACAGACCTGGCCCTGACCTTCTCCTTG
CAGTATCCTCACTTCTTGAAGAGGGAAGGCAACAAGCTTCAGATCATGCT
GCAGCGCAGAAAGCGCTACAAGAACAGAACCATCCTGGGCTACAAGACGC
TGGCCGCGGGCTCCATCAGCATGGCTGAGGTGATGCAACACCCGTCTGAA
GGTGGCCAGGTGCTGAGCCTCTGCAGCAGCATCAAGGAGGCCCCCGTCAA
GGCGGCCGAGATCTGGATCGCCTCCCTGTCCAGCCAGCCCATTGACCACG
AAGACAGCACCATGCAGGCCGGCCCCAAGGCCAAGTCCACGGATAACTAC
TCCGAGGAGGAGTATGAGAGCTTCTCCTCCGAGCAGGAGGCCAGTGACGA
CGCCGTGCAGGGGCAGGACTTGGACGAGGACGACTTTGACGTGGGGAAGC
CGAAGAAGCAGCGGAGATCGATTGTAAGAACGACGTCCATGACCAGGCAA
CAGAACTTCAAGCAGAAAGTGGTAGCGCTGCTGCGGAGGTTCAAAGTGTC
CGACGAGGTCCTGGACTCGGAGCAGGACCCTGCGGAGCACATCCCCGAGG
CAGAGGAGGACCTGGACCTCCTGTATGACACCCTGGACATGGAGCACCCC
AGCGACAGCGGCCCCGACATGGAGGATGACGACAGCGTCCTCAGCACCCC
CAAGCCGAAGCTGCGGCCATACTTTGAAGGCCTGTCGCACTCGAGCTCGC
AGACGGAGATTGGGAGCATCCACAGCGCCCGCAGCCACAAGGAGCCCCCA
AGCCCGGCTGACGTGCCCGAGAAGACGCGGTCCCTGGGAGGCAGGCAGCC
GAGCGACAGTGTCTCTGACACGGTGGCCCTCGGTGTGCCAGGCCCGAGGG
AGCACCCTGGACAGCCTGAGGACAGCCCCGAGGCTGAGGCCTCCACCCTG
GATGTGTTCACGGAGAGGCTGCCGCCCAGCGGGAGGATCACCAAGACAGA
GTCCCTTGTCATCCCCTCCACCAGGTCCGAGGGGAAGCAGGCTGGCCGAC
GGGGCCGGAGCACAGCCTTGAAGGAGCGGCAGGCAGCACGGCCCCAGAAT
GAGCGGGCCAACAGCCTGGACAACGAGCGCTGCCCGGACGCCCGGAGCCA
GCTACAGATCCCCAGGAAGACTGTGTATGACCAGCTCAACCACATCCTCA
TCTCCGATGACCAGCTTCCCGAAAACATCATCCTTGTCAACACCTCGGAC
TGGCAGGGGCAGTTCCTCTCCGACGTCCTGCAGAGGCACACGCTCCCCGT
GGTGTGCACGTGCTCTCCTGCGGACGTCCAGGCGGCCTTCAGCACCATCG
TCTCACGGATACAGAGATACTGCAACTGCAATTCCCAGCCCCCGACCCCC
GTGAAGATCGCCGTGGCGGGAGCGCAGCATTACCTCAGTGCCATCCTGCG
GCTCTTTGTGGAGCAGCTGTCCCACAAGACACCCGACTGGCTCGGCTACA
TGCGCTTCCTGGTCATCCCACTGGGCTCCCACCCCGTGGCCAGGTACCTA
GGCTCCGTGGACTACCGCTACAACAACTTCTTCCAGGACCTGGCCTGGAG
AGACCTGTTCAACAAGCTGGAGGCCCAGAGTGCGGTACAGGACACGCCAG
ACATTGTGTCACGCATCACGCAGTACATCGCAGGGGCCAACTGTGCCCAC
CAGCTCCCCATCGCAGAGGCCATGCTGACCTACAAGCAGAAGAGCCCTGA
CGAAGAGTCCTCCCAAAAGTTCATTCCCTTTGTCGGGGTTGTGAAGGTTG
GAATTGTGGAGCCATCCTCGGCCACATCAGGCGACTCGGACGACGCGGCC
CCCTCGGGCTCTGGCACGCTCTCCTCCACCCCGCCGTCCGCATCTCCTGC
GGCCAAGGAGGCCTCACCCACCCCGCCCTCCTCCCCGTCGGTGAGCGGAG
GCCTGTCCTCCCCCAGCCAGGGTGTCGGCGCCGAGCTGATGGGGCTGCAG
GTGGACTACTGGACGGCAGCACAGCCTGCGGACAGGAAGAGGGACGCCGA
GAAGAAGGACCTGCCTGTCACCAAAAACACGCTCAAGTGCACTTTCCGGT
CCCTCCAGGTCAGCAGGCTGCCCAGCAGCGGCGAGGCTGCAGCCACGCCC
ACCATGTCCATGACCGTGGTCACCAAGGAGAAGAACAAGAAGGTGATGTT
TCTGCCCAAGAAAGCGAAGGACAAGGACGTGGAGTCTAAGAGCCAGTGCA
TTGAGGGCATCAGCCGGCTCATCTGCACTGCCAGGCAGCAGCAGAACATG
CTGCGGGTCCTCATCGACGGCGTGGAGTGCAGCGACGTCAAGTTCTTCCA
GCTGGCCGCGCAGTGGTCCTCGCACGTGAAGCACTTCCCCATCTGCATCT
TCGGACACTCCAAGGCCACCTTCTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMCQAAHPSDCNCT
LASHVTAPPPSARPARPPRVRGPAAAPGRRGSGGAGAMAERGRLGLPGAP
GALNTPVPMNLFATWEVDGSSPSCVPRLCSLTLKKLVVFKELEKELISVV
IAVKMQGSKRILRSHEIVLPPSGQVETDLALTFSLQYPHFLKREGNKLQI
MLQRRKRYKNRTILGYKTLAAGSISMAEVMQHPSEGGQVLSLCSSIKEAP
VKAAEIWIASLSSQPIDHEDSTMQAGPKAKSTDNYSEEEYESFSSEQEAS
DDAVQGQDLDEDDFDVGKPKKQRRSIVRTTSMTRQQNFKQKVVALLRRFK
VSDEVLDSEQDPAEHIPEAEEDLDLLYDTLDMEHPSDSGPDMEDDDSVLS
TPKPKLRPYFEGLSHSSSQTEIGSIHSARSHKEPPSPADVPEKTRSLGGR
QPSDSVSDTVALGVPGPREHPGQPEDSPEAEASTLDVFTERLPPSGRITK
TESLVIPSTRSEGKQAGRRGRSTALKERQAARPQNERANSLDNERCPDAR
SQLQIPRKTVYDQLNHILISDDQLPENIILVNTSDWQGQFLSDVLQRHTL
PVVCTCSPADVQAAFSTIVSRIQRYCNCNSQPPTPVKIAVAGAQHYLSAI
LRLFVEQLSHKTPDWLGYMRFLVIPLGSHPVARYLGSVDYRYNNFFQDLA
WRDLFNKLEAQSAVQDTPDIVSRITQYIAGANCAHQLPIAEAMLTYKQKS
PDEESSQKFIPFVGVVKVGIVEPSSATSGDSDDAAPSGSGTLSSTPPSAS
PAAKEASPTPPSSPSVSGGLSSPSQGVGAELMGLQVDYWTAAQPADRKRD
AEKKDLPVTKNTLKCTFRSLQVSRLPSSGEAAATPTMSMTVVTKEKNKKV
MFLPKKAKDKDVESKSQCIEGISRLICTARQQQNMLRVLIDGVECSDVKF
FQLAAQWSSHVKHFPICIFGHSKATF

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7091
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express (S487A) mutated PACS2 with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 129984 Daltons