Plasmid
pEBG6P-MIZ1
Parent Plasmid
pEBG6P
Expressed
GST-MIZ1 (Pias2)
Genbank
NM_008602
Tag
GST
Species
Mouse
Sequence of Insert
View Sequence
ggtaccATGGTTTCTAGTTTTAGGGTTTCTGAGTTACAAGTGTTACTGGGCTTTGCTGGACGGAATAAAAGTGGGCGCAAGCATGACCTCCTGATGAGGGCGTTGCATTTATTGAAGAGTGGCTGCAGCCCTGCGGTTCAGATTAAAATTCGAGAATTATATAGACGCCGATACCCACGGACACTTGAAGGACTTTGTGATCTTTCCACAATCAAATCATCGGTTTTCAGCTTGGATGGTAGCTCATCACCCGTAGAACCTGACTTGCCTGTGGCTGGGATCCACTCGTTGCCTTCGACTTCAATTACACCTCATTCACCGTCATCTCCTGTTGGTTCTGTACTGCTGCAAGACACTAAGCCCACCTTTGAGATGCAGCAGCCGTCTCCGCCCATTCCTCCTGTCCATCCTGATGTGCAGTTAAAAAACCTGCCTTTCTATGATGTCCTTGATGTTCTCATCAAGCCCACGAGTTTAGTTCAAAGCAGTATTCAGCGGTTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAGCAAGTTAGAGAAATATGTATTTCAAGGGACTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAGCTGCGACTTTGCTTGGCAGAGACCAGTTGCCCTCAAGAAGATAACTATCCCAATAGTTTGTGTATAAAAGTCAATGGGAAACTCTTTCCTTTGCCTGGCTATGCGCCACCACCTAAAAATGGGATTGAACAGAAGCGCCCTGGACGCCCCCTGAACATTACGTCTTTAGTTAGGTTGTCTTCAGCTGTGCCAAATCAGATTTCTATTTCTTGGGCATCTGAAATTGGAAAGAATTACTCCATGTCTGTGTATCTTGTACGACAGCTTACATCAGCCATGTTATTACAGAGATTAAAAATGAAAGGTATTAGAAATCCTGATCATTCCAGAGCACTAATTAAAGAAAAACTTACTGCAGACCCTGATAGTGAAATTGCTACAACTAGTCTTCGAGTGTCCTTGATGTGCCCTTTAGGAAAAATGAGGCTGACAATCCCGTGCCGTGCAGTGACTTGTACACATCTGCAGTGCTTTGATGCTGCCCTCTATCTTCAGATGAATGAGAAAAAGCCCACCTGGATCTGTCCTGTTTGTGACAAAAAGGCTGCCTATGAGAGTCTGATACTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTGGATGAGATCAAATTCCAGGAAGATGGTTCTTGGTGTCCCATGAGACCTAAAAAAGAAGCTATGAAAGTAACCAGCCAGCCCTGTACAAAAGTAGAAAGTTCAAGTGTCTTTAGTAAACCTTGTTCAGTGACTGTAGCCAGTGATGCAAGCAAGAAGAAGATAGATGTTATTGATCTAACAATAGAGAGCTCTTCTGATGAAGAGGAAGACCCTCCCGCCAAAAGGAAATGCATCTTTATGTCAGAAACACAAAGCAGTCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGGTGCCCAGTGTGACTTCAGTTGATCCTGCTGCTATTCCACCTTCATTAACAGACTACTCAGTACCATTCCACCACACGCCAGTGTCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGATCCCCAGTACTGTCCTCCTATGTTTTTGGATAGTCTCACCTCACCCTTAACAGCAAGCAGTACGTCTGTCACCACCACCAGCCCCCATGAAAGCAGTACTCACGTTAGTTCATCCAGCAGCAGGAGTGAGACAGGGGTCATAACCAGCAGTGGGAGGAACATTCCTGACATCATCTCTTTGGACTAAgcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLCDLSTIKSSVFSLDGSSSPVEPDLPVAGIHSLPSTSITPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVTSQPCTKVESSSVFSKPCSVTVASDASKKKIDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPVSSMSSDLPGLDFLSLIPVDPQYCPPMFLDSLTSPLTASSTSVTTTSPHESSTHVSSSSSRSETGVITSSGRNIPDIISLD
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU7007
Unit Source
Location
PPU
MTA
Notes
Mammalian expression plasmid to express Miz1 with N-terminal GST (contains precission protease site)
IMAGE clones 3470677 and 3470495 have additional N-terminal MADFEELRN sequence and a R to G mutation at position 164 of the Miz1 sequence shown here.
Estimated MW of expressed protein : 95038 Daltons
IMAGE clones 3470677 and 3470495 have additional N-terminal MADFEELRN sequence and a R to G mutation at position 164 of the Miz1 sequence shown here.
Estimated MW of expressed protein : 95038 Daltons
Price
£125.00
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