cDNA Clone TBK1

• Synonyms: *
• Plasmid: pGEX6P-1-TBK1
• Parent Plasmid: pGEX6P1
• Expressed: GST-PreScission-TANK-binding kinase 1 (TBK1)
• Genbank: NP_037386
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTT
AGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTG
GTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCA
GTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAA
AAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGACATA
AAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTA
GAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGT
TTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAG
TGCACCGTGATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGAT
GGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAATTAGA
AGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACC
CTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATAT
GGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGC
TACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAG
AAGTGATGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGA
GTACAGAAAGCAGAAAATGGACCAATTGACTGGAGTGGAGACATGCCTGT
TTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTTCTTG
CAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTT
TTTGCAGAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTC
GCTACAACAAATGACAGCTCATAAGATTTATATACATAGCTATAATACTG
CTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCA
AATCAAGAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAG
GCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTTGTAG
TAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCC
CTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGC
TAAGGCAATAACAGGGGTTGTGTGTTATGCCTGCAGAATTGCCAGTACCT
TACTGCTTTATCAGGAATTAATGCGAAAGGGGATACGATGGCTGATTGAA
TTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGT
GATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTAT
ATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATT
TCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAAT
AGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCAC
TGGCAGACGCATGGGCACATCAAGAAGGCACTCATCCGAAAGACAGAAAT
GTAGAAAAACTACAAGTCCTGTTAAATTGCATGACAGAGATTTACTATCA
GTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAA
TCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATG
ACGCACTTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAA
GTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTATTAT
CGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAA
GAATATACTAATGAGTTACAAGAAACTCTGCCTCAGAAAATGTTTACAGC
TTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCTAACA
CATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAA
GGGGTGGTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGG
CTCTTTAACCATGGATGGTGGCCTTCGCAACGTTGACTGTCTTTAGGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLD
IRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHP
DFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAW
PLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQSTSNHLWLLSDILGQGAT
ANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKL
FAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVV
GGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQF
VSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLP
FRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLS
RGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMT
AHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHF
PKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITG
VVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDF
CIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQ
DIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDK
AERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWI
RKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIK
HTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMD
GGLRNVDCL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-TBK1126.17 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU9001
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminal GST; for bacterial expression.; ; Estimated MW of expressed protein : 41843 Daltons
• Low Temp: No