cDNA Clone UBE2J1

• Plasmid: pET28(+) UBE2J1
• Parent Plasmid: pET28a original
• Expressed: 6H-UBE2J1
• Genbank: NM_016021
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAGACCCGCTACAACCTGAAGAGTCCGGCTGTTAAACGTTT
AATGAAAGAAGCGGCAGAATTGAAAGATCCAACAGATCATTACCATGCGC
AGCCTTTAGAGGATAACCTTTTTGAATGGCACTTCACGGTTAGAGGGCCC
CCAGACTCCGATTTTGATGGAGGAGTTTATCACGGGCGGATAGTACTGCC
ACCAGAGTATCCCATGAAACCACCAAGCATTATTCTCCTAACGGCTAATG
GTCGATTTGAAGTGGGCAAGAAAATCTGTTTGAGCATCTCAGGCCATCAT
CCTGAAACTTGGCAGCCTTCGTGGAGTATAAGGACAGCATTATTAGCCAT
CATTGGGTTTATGCCAACAAAAGGAGAGGGAGCCATAGGTTCTCTAGATT
ACACTCCTGAGGAAAGAAGAGCACTTGCCAAAAAATCACAAGATTTCTGT
TGTGAAGGATGTGGCTCTGCCATGAAGGATGTCCTGTTGCCTTTAAAATC
TGGAAGCGATTCAAGCCAAGCTGACCAAGAAGCCAAAGAACTGGCTAGGC
AAATAAGCTTTAAGGCAGAAGTCAATTCATCTGGAAAGACTATCTCTGAG
TCAGACTTAAACCACTCTTTTTCACTAACTGATTTACAAGATGATATACC
TACAACATTCCAGGGTGCTACGGCCAGTACATCGTACGGACTCCAGAATT
CCTCAGCAGCATCCTTTCATCAACCTACCCAACCTGTAGCTAAGAATACC
TCCATGAGCCCTCGACAGCGCCGGGCCCAGCAGCAGAGTCAGAGAAGGTT
GTCTACTTCACCAGATGTAATCCAGGGCCACCAGCCAAGAGACAACCACA
CTGATCATGGTGGGTCAGCTGTACTGATTGTCATCCTGACTTTGGCATTG
GCAGCTCTTATATTCCGACGAATATATCTGGCAAACGAATACATATTTGA
CTTTGAGTTATAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMETRYNLKSPAVKRLM
KEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPP
EYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAII
GFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSG
SDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPT
TFQGATASTSYGLQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLS
TSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAALIFRRIYLANEYIFDF
EL*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28(+) UBE2J187.98 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU14077
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 38741 Daltons
• Low Temp: No