cDNA Clone UBE2G1

• Plasmid: pET28a(+) UBE2G1
• Parent Plasmid: pET28a original
• Expressed: 6H-UBE2G1
• Genbank: NM_003342
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACGGAGCTGCAGTCGGCACTGCTACTGCGAAGACAGCTGGC
AGAACTCAACAAAAATCCAGTGGAAGGCTTTTCTGCAGGTTTAATAGATG
ACAATGATCTCTACCGATGGGAAGTCCTTATTATTGGCCCTCCAGATACA
CTTTATGAAGGTGGTGTTTTTAAGGCTCATCTTACTTTCCCAAAAGATTA
TCCCCTCCGACCTCCTAAAATGAAATTCATTACAGAAATCTGGCACCCAA
ATGTTGATAAAAATGGTGATGTGTGCATTTCTATTCTTCATGAGCCTGGG
GAAGATAAGTATGGTTATGAAAAGCCAGAGGAACGCTGGCTCCCTATCCA
CACTGTGGAAACCATCATGATTAGTGTCATTTCTATGCTGGCAGACCCTA
ATGGAGACTCACCTGCTAATGTTGATGCTGCGAAAGAATGGAGGGAAGAT
AGAAATGGAGAATTTAAAAGAAAAGTTGCCCGCTGTGTAAGAAAAAGCCA
AGAGACTGCTTTTGAGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMTELQSALLLRRQLAE
LNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYP
LRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHT
VETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKSQE
TAFE*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU14055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 23052 Daltons