cDNA Clone NCC

• Plasmid: pSG5-FLAG-SLC12A3 (hNCC) T46A T50A T55A S91A
• Parent Plasmid: pSG5
• Expressed: FLAG-SLC12A3 (hNCC) T46A T50A T55A S91A
• Genbank: P55017
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGATGGCAGAACT
GCCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGGGCGCTTCA
CCATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGCCTAT
GACAGCAGCCACCCCAGCCACCTGGCCCACAGCAGCGCCTTCTGCATGCG
CGCCTTTGGCTATAACACGATCGATGTGGTGCCCACATATGAGCACTATG
CCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCACACTGGCT
GACCTGCACGCCTTCCTCAAGCAGGAAGGCAGACACCTGCATGCCCTGGC
CTTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGTGGAGGGCG
AGGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGCGCTTCGGC
TGGGTCAAGGGGGTGATGATTCGTTGCATGCTCAACATTTGGGGCGTGAT
CCTCTACCTGCGGCTGCCCTGGATTACGGCCCAGGCAGGCATCGTCCTGA
CCTGGATCATCATCCTGCTGTCGGTCACGGTGACCTCCATCACAGGCCTC
TCCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGTGGCACCTA
CTTCCTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTCCATCGGCC
TCATTTTCGCTTTCGCCAATGCCGTGGGTGTGGCCATGCACACGGTGGGC
TTTGCAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGGGCACCCATCGT
GGACCCCATTAACGACATCCGCATCATTGGCGTGGTCTCGGTCACTGTGC
TGCTGGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCAAGGCCCAGGTG
CTGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAACTATTTAGTGGGGAC
GCTGATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTTCAGCTACC
GGGCGGACATTTTTGTCCAAAACTTGGTGCCTGACTGGCGGGGTCCAGAT
GGCACCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCGGCCACAGGCAT
CCTGGCAGGGGCCAACATATCTGGTGACCTCAAGGACCCTGCTATAGCCA
TCCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTTCCTACCTG
GCCATCTCAGCCACCATTGGCTCCTGCGTGGTGCGTGATGCCTCTGGGGT
CCTGAATGACACAGTGACCCCTGGCTGGGGTGCCTGCGAGGGGCTGGCCT
GCAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACAGCTGCCAC
TACGGCCTCATCAACTATTACCAGACCATGAGCATGGTGTCAGGCTTCGC
GCCCCTGATCACGGCTGGCATCTTCGGGGCCACCCTCTCCTCTGCCCTGG
CCTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTTTGCGAGGACCAG
CTGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGCAAGAACAAGGA
GCCCGTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGCCTTCATCA
TCATCGCTGAGCTCAACACCATAGCCCCCATCATTTCCAACTTCTTCCTC
TGCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCCATCACCAA
CTCGCCTGGGTGGAGACCTTCATTCCAATACTACAACAAGTGGGCGGCGC
TGTTTGGGGCTATCATCTCCGTGGTCATCATGTTCCTCCTCACCTGGTGG
GCGGCCCTCATCGCCATTGGCGTGGTGCTCTTCCTCCTGCTCTATGTCAT
CTACAAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACAGGCTGGCTCCT
ACAACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGGAAGACCAC
ATCAAGAACTACCGCCCCCAGTGCCTGGTGCTCACGGGGCCCCCCAACTT
CCGCCCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCGGAACCTCAGCC
TGATGATCTGTGGCCACGTGCTCATCGGACCCCACAAGCAGAGGATGCCT
GAGCTCCAGCTCATCGCCAACGGGCACACCAAGTGGCTGAACAAGAGGAA
GATCAAGGCCTTCTACTCGGATGTCATTGCTGAGGACCTCCGCAGAGGCG
TCCAGATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGAAGCCCAACATT
CTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCGGCCACAGT
GGAAGACTACATTGGCATCCTCCATGATGCCTTTGATTTCAACTATGGCG
TGTGTGTCATGAGGATGCGGGAGGGACTCAACGTGTCCAAGATGATGCAG
GCGCACATTAACCCCGTGTTTGACCCAGCGGAGGACGGGAAGGAAGCCAG
CGCCAGAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGCCACCACCA
TCTTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACTGGCTCTTT
GACGATGGAGGCCTCACCCTCCTCATTCCCTATCTCCTTGGCCGCAAGAG
GAGGTGGAGCAAATGCAAGATCCGTGTGTTCGTAGGTGGCCAGATTAACA
GGATGGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGAGCAAGTTCCGG
CTGGGATTCCATGAAGTCCACATCCTCCCTGACATCAACCAGAACCCTCG
GGCTGAGCACACCAAGAGGTTTGAGGACATGATTGCACCCTTCCGTCTGA
ATGATGGCTTCAAGGATGAGGCCACTGTCAACGAGATGCGGCGGGACTGC
CCCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTCAAGTCCCT
TCGGCAGGTGAGGCTGAATGAGATTGTGCTGGATTACTCCCGAGACGCTG
CTCTCATCGTCATCACTTTGCCCATAGGGAGGAAGGGGAAGTGCCCCAGC
TCGCTGTACATGGCCTgGCTGGAGACCCTGTCCCAGGACCTCAGACCTCC
AGTCATCCTGATCCGAGGAAACCAGGAAAACGTGCTCACCTTTTACTGCC
AGTAAGGATCC

Amino Acid Sequence

MDYKDDDDKMAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSH
PSHLAHSSAFCMRAFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHA
FLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKG
VMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISA
ISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAET
VRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFL
VIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFF
GMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISA
TIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLI
NYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPL
IGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYA
LINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALI
AIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNY
RPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQL
IANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVG
FKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHIN
PVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGG
LTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFH
EVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKI
SDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYM
AWLETLSQDLRPPVILIRGNQENVLTFYCQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4856
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with FLAG tag.