cDNA Clone DAZAP1-b

• Plasmid: pAS2.1 DAZAP1-b 155-407 T269D T315D
• Parent Plasmid: pAS2.1
• Expressed: GAL4-DNA-BD-DAZAP1-b 155-407 T269D T315D
• Genbank: NM_018959
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAA
AAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGA
ACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACT
AGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCT
ATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGG
ATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGA
TATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGG
AAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGTATTG
CAATACCCAGCTTTGACTCATATGGGTTTTGGATTTATTACTTTCGAGGA
CGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGG
GCAAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAA
GCGCCGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAA
CGCTGCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGAT
ATGGCCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTAT
GGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCAC
CTCCTACATCGTGTCCGACCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCT
TCCCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCT
CCACCTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACC
AGCCGATCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGG
CTGCCCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTAT
GGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTT
CTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAG
GGTCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAAC
CACAACGTGCAAGGGTTCCACCCCTACCGACGCTAGGTCGAC

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVL
QYPALTHMGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQ
APGQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGY
GPPPAGRGAPPPPPPFTSYIVSDPPGGFPPPQGFPQGYGAPPQFSFGYGP
PPPPPDQFAPPGVPPPPADPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPY
GQYAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQN
HNVQGFHPYRR*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4746
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression vector with GAL4 DNA-BD fused to Dazap1-b.