cDNA Clone ASK1

• Synonyms: MAP3K5
• Plasmid: pGEX6P-3-ASK1 FL
• Parent Plasmid: pGEX6P3
• Expressed: GST-ASK1 FL
• Genbank: NM_005923
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATT
CCCGGGTCGACAGCACGGAGGCGGACGAGGGCATCACTTTCTCTGTGCCA
CCCTTCGCCCCCTCGGGCTTCTGCACCATCCCCGAGGGCGGCATCTGCAG
GAGGGGAGGAGCGGCGGCGGTGGGCGAGGGCGAGGAGCACCAGCTGCCAC
CGCCGCCGCCGGGCAGTTTCTGGAACGTGGAGAGCGCCGCTGCCCCTGGC
ATCGGTTGTCCGGCGGCCACCTCCTCGAGCAGTGCCACCCGAGGCCGGGG
CAGCTCTGTTGGCGGGGGCAGCCGACGGACCACGGTGGCATATGTGATCA
ACGAAGCGAGCCAAGGGCAACTGGTGGTGGCCGAGAGCGAGGCCCTGCAG
AGCTTGCGGGAGGCGTGCGAGACAGTGGGCGCCACCCTGGAAACCCTGCA
TTTTGGGAAACTCGACTTTGGAGAAACCACCGTGCTGGACCGCTTTTACA
ATGCAGATATTGCGGTGGTGGAGATGAGCGATGCCTTCCGGCAGCCGTCC
TTGTTTTACCACCTTGGGGTGAGAGAAAGTTTCAGCATGGCCAACAACAT
CATCCTCTACTGTGATACTAACTCGGACTCTCTGCAGTCACTGAAGGAAA
TCATTTGCCAGAAGAATACTATGTGCACTGGGAACTACACCTTTGTTCCT
TACATGATAACTCCACATAACAAAGTCTACTGCTGTGACAGCAGCTTCAT
GAAGGGGTTGACAGAGCTCATGCAACCGAACTTCGAGCTGCTTCTTGGAC
CCATCTGCTTACCTCTTGTGGATCGTTTTATTCAACTTTTGAAGGTGGCA
CAAGCAAGTTCTAGCCAGTACTTCCGGGAATCTATACTCAATGACATCAG
GAAAGCTCGTAATTTATACACTGGTAAAGAATTGGCAGCTGAGTTGGCAA
GAATTCGGCAGCGAGTAGATAATATCGAAGTCTTGACAGCAGATATTGTC
ATAAATCTGTTACTTTCCTACAGAGATATCCAGGACTATGATTCTATTGT
GAAGCTGGTAGAGACTTTAGAAAAACTGCCAACCTTTGATTTGGCCTCCC
ATCACCATGTGAAGTTTCATTATGCATTTGCACTGAATAGGAGAAATCTC
CCTGGTGACAGAGCAAAAGCTCTTGATATTATGATTCCCATGGTGCAAAG
CGAAGGACAAGTTGCTTCAGATATGTATTGCCTAGTTGGTCGAATCTACA
AAGATATGTTTTTGGACTCTAATTTCACGGACACTGAAAGCAGAGACCAT
GGAGCTTCTTGGTTCAAAAAGGCATTTGAATCTGAGCCAACACTACAGTC
AGGAATTAATTATGCGGTCCTCCTCCTGGCAGCTGGACACCAGTTTGAAT
CTTCCTTTGAGCTCCGGAAAGTTGGGGTGAAGCTAAGTAGTCTTCTTGGT
AAAAAGGGAAACTTGGAAAAACTCCAGAGCTACTGGGAAGTTGGATTTTT
TCTGGGGGCCAGCGTCCTAGCCAATGACCACATGAGAGTCATTCAAGCAT
CTGAAAAGCTTTTTAAACTGAAGACACCAGCATGGTACCTCAAGTCTATT
GTAGAGACAATTTTAATATATAAGCATTTTGTGAAACTGACCACAGAACA
GCCTGTGGCCAAGCAAGAACTTGTGGACTTTTGGATGGATTTCCTGGTCG
AGGCCACAAAGACAGATGTTACTGTGGTTAGGTTTCCAGTATTAATATTA
GAACCAACCAAAATCTATCAACCTTCTTATTTGTCTATCAACAATGAAGT
TGAGGAAAAGACAATCTCTATTTGGCACGTGCTTCCTGATGACAAGAAAG
GTATACATGAGTGGAATTTTAGTGCCTCTTCTGTCAGGGGAGTGAGTATT
TCTAAATTTGAAGAAAGATGCTGCTTTCTTTATGTGCTTCACAATTCTGA
TGATTTCCAAATCTATTTCTGTACAGAACTTCATTGTAAAAAGTTTTTTG
AGATGGTGAACACCATTACCGAAGAGAAGGGGAGAAGCACAGAGGAAGGA
GACTGTGAAAGTGACTTGCTGGAGTATGACTATGAATATGATGAAAATGG
TGACAGAGTCGTTTTAGGAAAAGGCACTTATGGGATAGTCTACGCAGGTC
GGGACTTGAGCAACCAAGTCAGAATTGCTATTAAGGAAATCCCAGAGAGA
GACAGCAGATACTCTCAGCCCCTGCATGAAGAAATAGCATTGCATAAACA
CCTGAAGCACAAAAATATTGTCCAGTATCTGGGCTCTTTCAGTGAGAATG
GTTTCATTAAAATCTTCATGGAGCAGGTCCCTGGAGGAAGTCTTTCTGCT
CTCCTTCGTTCCAAATGGGGTCCATTAAAAGACAATGAGCAAACAATTGG
CTTTTATACAAAGCAAATACTGGAAGGATTAAAATATCTCCATGACAATC
AGATAGTTCACCGGGACATAAAGGGTGACAATGTGTTGATTAATACCTAC
AGTGGTGTTCTCAAGATCTCTGACTTCGGAACATCAAAGAGGCTTGCTGG
CATAAACCCCTGTACTGAAACTTTTACTGGTACCCTCCAGTATATGGCAC
CAGAAATAATAGATAAAGGACCAAGAGGCTACGGAAAAGCAGCAGACATC
TGGTCTCTGGGCTGTACAATCATTGAAATGGCCACAGGAAAACCCCCATT
TTATGAACTGGGAGAACCACAAGCAGCTATGTTCAAGGTGGGAATGTTTA
AAGTCCACCCTGAGATCCCAGAGTCCATGTCTGCAGAGGCCAAGGCATTC
ATACTGAAATGTTTTGAACCAGATCCTGACAAGAGAGCCTGTGCTAACGA
CTTGCTTGTTGATGAGTTTTTAAAAGTTTCAAGCAAAAAGAAAAAGACAC
AACCTAAGCTTTCAGCTCTTTCAGCTGGATCAAATGAATATCTCAGGAGT
ATATCCTTGCCGGTACCTGTGCTGGTGGAGGACACCAGCAGCAGCAGTGA
GTACGGCTCAGTTTCACCCGACACGGAGTTGAAAGTGGACCCCTTCTCTT
TCAAAACAAGAGCCAAGTCCTGCGGAGAAAGAGATGTCAAGGGAATTCGG
ACACTCTTTTTGGGCATTCCAGATGAGAATTTTGAAGATCACAGTGCTCC
TCCTTCCCCTGAAGAAAAAGATTCTGGATTCTTCATGCTGAGGAAGGACA
GTGAGAGGCGAGCTACCCTTCACAGGATCCTGACGGAAGACCAAGACAAA
ATTGTGAGAAACCTAATGGAATCTTTAGCTCACGGGGCTGAAGAACCGAA
ACTAAAATGGGAACACATCACAACCCTCATTGCAAGCCTCAGAGAATTTG
TGAGATCCACTGACCGAAAAATCATAGCCACCACACTGTCAAAGCTGAAA
CTGGAGCTGGACTTCGACAGCCATGGCATTAGCCAAGTCCAGGTGGTACT
CTTTGGTTTTCAAGATGCTGTCAATAAAGTTCTTCGGAATCATAACATCA
AGCCGCACTGGATGTTTGCCTTAGACAGTATCATTCGGAAGGCGGTACAG
ACAGCCATTACCATCCTGGTTCCAGAACTAAGGCCACATTTCAGCCTTGC
ATCTGAGAGTGATACTGCTGATCAAGAAGACTTGGATGTAGAAGATGACC
ATGAGGAACAGCCTTCAAATCAAACTGTCCGAAGACCTCAGGCTGTCATT
GAAGATGCTGTGGCTACCTCAGGCGTGAGCACGCTCAGTTCTACTGTGTC
TCATGATTCCCAGAGTGCTCACCGGTCACTGAATGTACAGCTTGGAAGGA
TGAAAATAGAAACCAATAGATTACTGGAAGAATTGGTTCGGAAAGAGAAA
GAATTACAAGCACTCCTTCATCGAGCTATTGAAGAAAAAGACCAAGAAAT
TAAACACCTGAAGCTTAAGTCCCAACCCATAGAAATTCCTGAATTGCCTG
TATTTCATCTAAATTCTTCTGGCACAAATACTGAAGATTCTGAACTTACC
GACTGGCTGAGAGTGAATGGAGCTGATGAAGACACTATAAGCCGGTTTTT
GGCTGAAGATTATACACTATTGGATGTTCTCTACTATGTTACACGTGATG
ACTTAAAATGCTTGAGACTAAGGGGAGGGATGCTGTGCACACTGTGGAAG
GCTATCATTGACTTTCGAAACAAACAGACTTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDSTEADEGITFSVP
PFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPG
IGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQ
SLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPS
LFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVP
YMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVA
QASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIV
INLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNL
PGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDH
GASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLG
KKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSI
VETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLIL
EPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSI
SKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEG
DCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPER
DSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSA
LLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTY
SGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADI
WSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAF
ILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRS
ISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIR
TLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDK
IVRNLMESLAHGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLK
LELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQ
TAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVI
EDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEK
ELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELT
DWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWK
AIIDFRNKQT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4707
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.