cDNA Clone NCC

• Plasmid: pNEBRX1 Hygro FLAG SLC12A3 (hNCC) T46A T50A T55A
• Parent Plasmid: pNEBRX1
• Expressed: FLAG-SLC12A3 (hNCC) T46A T50A T55A
• Genbank: P55017
• Tag: FLAG
• Species: Human

Sequence of Insert

CCCGCCCCCGGCGGATACTGTCCTCCGAGCGAATACTGTCCTCCGAGCGG
AGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGTTCTGTCCTC
CGAGCGGAGACTCTAGAGGGTATATAATGGGGGCCGTCAGCTACTACCAG
AGCTCATGGGCCCGTGCAATTGAAGCCGGCTGGCGCCAAGCTTCTCTGCA
GGATATCTGGATCCGCCACCATGGACTACAAGGACGACGATGACAAGATG
GCAGAACTGCCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGG
GCGCTTCACCATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAG
CTGCCTATGACAGCAGCCACCCCAGCCACCTGGCCCACAGCAGCGCCTTC
TGCATGCGCGCCTTTGGCTATAACACGATCGATGTGGTGCCCACATATGA
GCACTATGCCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCA
CACTGGCTGACCTGCACTCCTTCCTCAAGCAGGAAGGCAGACACCTGCAT
GCCCTGGCCTTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGT
GGAGGGCGAGGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGC
GCTTCGGCTGGGTCAAGGGGGTGATGATTCGTTGCATGCTCAACATTTGG
GGCGTGATCCTCTACCTGCGGCTGCCCTGGATTACGGCCCAGGCAGGCAT
CGTCCTGACCTGGATCATCATCCTGCTGTCGGTCACGGTGACCTCCATCA
CAGGCCTCTCCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGT
GGCACCTACTTCCTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTC
CATCGGCCTCATTTTCGCTTTCGCCAATGCCGTGGGTGTGGCCATGCACA
CGGTGGGCTTTGCAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGGGCA
CCCATCGTGGACCCCATTAACGACATCCGCATCATTGGCGTGGTCTCGGT
CACTGTGCTGCTGGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCAAGG
CCCAGGTGCTGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAACTATTTA
GTGGGGACGCTGATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTT
CAGCTACCGGGCGGACATTTTTGTCCAAAACTTGGTGCCTGACTGGCGGG
GTCCAGATGGCACCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCGGCC
ACAGGCATCCTGGCAGGGGCCAACATATCTGGTGACCTCAAGGACCCTGC
TATAGCCATCCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTT
CCTACCTGGCCATCTCAGCCACCATTGGCTCCTGCGTGGTGCGTGATGCC
TCTGGGGTCCTGAATGACACAGTGACCCCTGGCTGGGGTGCCTGCGAGGG
GCTGGCCTGCAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACA
GCTGCCACTACGGCCTCATCAACTATTACCAGACCATGAGCATGGTGTCA
GGCTTCGCGCCCCTGATCACGGCTGGCATCTTCGGGGCCACCCTCTCCTC
TGCCCTGGCCTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTTTGCG
AGGACCAGCTGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGCAAG
AACAAGGAGCCCGTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGC
CTTCATCATCATCGCTGAGCTCAACACCATAGCCCCCATCATTTCCAACT
TCTTCCTCTGCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCC
ATCACCAACTCGCCTGGGTGGAGACCTTCATTCCAATACTACAACAAGTG
GGCGGCGCTGTTTGGGGCTATCATCTCCGTGGTCATCATGTTCCTCCTCA
CCTGGTGGGCGGCCCTCATCGCCATTGGCGTGGTGCTCTTCCTCCTGCTC
TATGTCATCTACAAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACAGGC
TGGCTCCTACAACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGG
AAGACCACATCAAGAACTACCGCCCCCAGTGCCTGGTGCTCACGGGGCCC
CCCAACTTCCGCCCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCGGAA
CCTCAGCCTGATGATCTGTGGCCACGTGCTCATCGGACCCCACAAGCAGA
GGATGCCTGAGCTCCAGCTCATCGCCAACGGGCACACCAAGTGGCTGAAC
AAGAGGAAGATCAAGGCCTTCTACTCGGATGTCATTGCTGAGGACCTCCG
CAGAGGCGTCCAGATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGAAGC
CCAACATTCTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCG
GCCACAGTGGAAGACTACATTGGCATCCTCCATGATGCCTTTGATTTCAA
CTATGGCGTGTGTGTCATGAGGATGCGGGAGGGACTCAACGTGTCCAAGA
TGATGCAGGCGCACATTAACCCCGTGTTTGACCCAGCGGAGGACGGGAAG
GAAGCCAGCGCCAGAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGC
CACCACCATCTTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACT
GGCTCTTTGACGATGGAGGCCTCACCCTCCTCATTCCCTATCTCCTTGGC
CGCAAGAGGAGGTGGAGCAAATGCAAGATCCGTGTGTTCGTAGGTGGCCA
GATTAACAGGATGGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGAGCA
AGTTCCGACTGGGATTCCATGAAGTCCACATCCTCCCTGACATCAACCAG
AACCCTCGGGCTGAGCACACCAAGAGGTTTGAGGACATGATTGCACCCTT
CCGTCTGAATGATGGCTTCAAGGATGAGGCCACTGTCAACGAGATGCGGC
GGGACTGCCCCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTC
AAGTCCCTTCGGCAGGTGAGGCTGAATGAGATTGTGCTGGATTACTCCCG
AGACGCTGCTCTCATCGTCATCACTTTGCCCATAGGGAGGAAGGGGAAGT
GCCCCAGCTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTC
AGACCTCCAGTCATCCTGATCCGAGGAAACCAGGAAAACGTGCTCACCTT
TTACTGCCAGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKMAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSH
PSHLAHSSAFCMRAFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHS
FLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKG
VMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISA
ISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAET
VRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFL
VIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFF
GMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISA
TIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLI
NYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPL
IGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYA
LINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALI
AIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNY
RPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQL
IANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVG
FKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHIN
PVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGG
LTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFH
EVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKI
SDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYM
AWLETLSQDLRPPVILIRGNQENVLTFYCQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4647
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal FLAG.