cDNA Clone PAK2

• Plasmid: pGEX6P-1-PAK2 G258R T402E
• Parent Plasmid: pGEX6P1
• Expressed: GST-PAK2 G258R T402E
• Genbank: BC069613
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGTCTG
ATAACGGAGAACTGGAAGATAAGCCTCCAGCACCTCCTGTGCGAATGAGC
AGCACCATCTTTAGCACTGGAGGCAAAGACCCTTTGTCAGCCAATCACAG
TTTGAAACCTTTGCCCTCTGTTCCAGAAGAGAAAAAGCCCAGGCATAAAA
TCATCTCCATATTCTCAGGCACAGAGAAAGGAAGTAAAAAGAAGGAAAAG
GAACGGCCAGAAATTTCTCCTCCATCTGATTTTGAGCACACCATCCATGT
TGGTTTTGATGCTGTTACTGGAGAATTCACTGGCATGCCAGAACAGTGGG
CTCGATTACTACAGACCTCCAATATCACCAAACTAGAGCAAAAGAAGAAC
CCTCAGGCTGTGCTGGATGTCCTAAAGTTCTACGACTCCAACACAGTGAA
GCAGAAATATCTGAGCTTTACTCCTCCTGAGAAAGATGGCTTTCCTTCTG
GAACACCAGCACTGAATGCCAAGGGAACAGAAGCACCCGCAGTAGTGACA
GAGGAGGAGGATGATGATGAAGAGACTGCTCCTCCCGTTATTGCCCCGCG
ACCGGATCATACGAAATCAATTTACACACGGTCTGTAATTGACCCTGTTC
CTGCACCAGTTGGTGATTCACATGTTGATGGTGCTGCCAAGTCTTTAGAC
AAACAGAAAAAGAAGACTAAGATGACAGATGAAGAGATTATGGAGAAATT
AAGAACTATCGTGAGCATAGGTGACCCTAAGAAAAAATATACAAGATATG
AAAAAATTGGACAAAGGGCTTCTGGTACAGTTTTCACTGCTACTGACGTT
GCACTGGGACAGGAGGTTGCTATCAAACAAATTAATTTACAGAAACAGCC
AAAGAAGGAACTGATCATTAACGAGATTCTGGTGATGAAAGAATTGAAAA
ATCCCAACATCGTTAACTTTTTGGACAGTTACCTGGTAGGAGATGAATTG
TTTGTGGTCATGGAATACCTTGCTGGGGGGTCACTCACTGATGTGGTAAC
AGAAACGTGCATGGATGAAGCACAGATTGCTGCTGTATGCAGAGAGTGTT
TACAGGCATTGGAGTTTTTACATGCTAATCAAGTGATCCACAGAGACATC
AAAAGTGACAATGTACTTTTGGGAATGGAAGGATCTGTTAAGCTCACTGA
CTTTGGTTTCTGTGCCCAGATCACCCCTGAGCAGAGCAAACGCAGTGAGA
TGGTCGGAACGCCATACTGGATGGCACCAGAGGTGGTTACACGGAAAGCT
TATGGCCCTAAAGTCGACATATGGTCTCTGGGTATCATGGCTATTGAGAT
GGTAGAAGGAGAGCCTCCATACCTCAATGAAAATCCCTTGAGGGCCTTGT
ACCTAATAGCAACTAATGGAACCCCAGAACTTCAGAATCCAGAGAAACTT
TCCCCAATATTTCGGGATTTCTTAAATCGATGTTTGGAAATGGATGTGGA
AAAAAGGGGTTCAGCCAAAGAATTATTACAGCATCCTTTCCTGAAACTGG
CCAAACCGTTATCTAGCTTGACACCACTGATCATGGCAGCTAAAGAAGCA
ATGAAGAGTAACCGTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSDNGELEDKPPAPPVRMS
STIFSTGGKDPLSANHSLKPLPSVPEEKKPRHKIISIFSGTEKGSKKKEK
ERPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKN
PQAVLDVLKFYDSNTVKQKYLSFTPPEKDGFPSGTPALNAKGTEAPAVVT
EEEDDDEETAPPVIAPRPDHTKSIYTRSVIDPVPAPVGDSHVDGAAKSLD
KQKKKTKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQRASGTVFTATDV
ALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDEL
FVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDI
KSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSEMVGTPYWMAPEVVTRKA
YGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKL
SPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEA
MKSNR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4558
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.; Note: This sequence differs from the genomic sequence of PAK2 because there is a G258R mutation in this particular accession.