cDNA Clone PACT

• Plasmid: pEBG6P-1 PACT
• Parent Plasmid: pEBG6P
• Expressed: GST-PACT
• Genbank: AF072860
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGTCCCAGAGCAGGCACCGCGCCGAGGCCCCGCCGCTG
GAGCGCGAGGACAGTGGGACCTTCAGTTTGGGGAAGATGATAACAGCTAA
GCCAGGGAAAACACCGATTCAGGTATTACACGAATACGGCATGAAGACCA
AGAACATCCCAGTTTATGAATGTGAAAGATCTGATGTGCAAATACACGTG
CCCACTTTCACCTTCAGAGTAACCGTTGGTGACATAACCTGCACAGGTGA
AGGTACAAGTAAGAAGCTGGCGAAACATAGAGCTGCAGAGGCTGCCATAA
ACATTTTGAAAGCCAATGCAAGTATTTGCTTTGCAGTTCCTGACCCCTTA
ATGCCTGACCCTTCCAAGCAACCAAAGAACCAGCTTAATCCTATTGGTTC
ATTACAGGAATTGGCTATTCATCATGGCTGGAGACTTCCTGAATATACCC
TTTCCCAGGAGGGAGGACCTGCTCATAAGAGAGAATATACTACAATTTGC
AGGCTAGAGTCATTTATGGAAACTGGAAAGGGGGCATCAAAAAAGCAAGC
CAAAAGGAATGCTGCTGAGAAATTTCTTGCCAAATTTAGTAATATTTCTC
CAGAGAACCACATTTCTTTAACAAATGTAGTAGGACATTCTTTAGGATGT
ACTTGGCATTCCTTGAGGAATTCTCCTGGTGAAAAGATCAACTTACTGAA
AAGAAGCCTCCTTAGTATTCCAAATACAGATTACATCCAGCTGCTTAGTG
AAATTGCCAAGGAACAAAGTTTTAATATAACATATTTGGATATAGATGAA
CTGAGCGCCAATGGACAATATCAATGTCTTGCTGAACTGTCCACCAGCCC
CATCACAGTCTGTCATGGCTCCGGTATCTCCTGTGGCAATGCACAAAGTG
ATGCAGCTCACAATGCTTTGCAGTATTTAAAGATAATAGCAGAAAGAAAG
TAAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSQSRHRAEAPPL
EREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHV
PTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPL
MPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTIC
RLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGC
TWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQSFNITYLDIDE
LSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4523
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.