cDNA Clone PAK2

• Plasmid: pCMV5-HA-1 PAK2 G258R
• Parent Plasmid: pCMV5
• Expressed: HA-PAK2 G258R
• Genbank: BC069613
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGTCTGA
TAACGGAGAACTGGAAGATAAGCCTCCAGCACCTCCTGTGCGAATGAGCA
GCACCATCTTTAGCACTGGAGGCAAAGACCCTTTGTCAGCCAATCACAGT
TTGAAACCTTTGCCCTCTGTTCCAGAAGAGAAAAAGCCCAGGCATAAAAT
CATCTCCATATTCTCAGGCACAGAGAAAGGAAGTAAAAAGAAGGAAAAGG
AACGGCCAGAAATTTCTCCTCCATCTGATTTTGAGCACACCATCCATGTT
GGTTTTGATGCTGTTACTGGAGAATTCACTGGCATGCCAGAACAGTGGGC
TCGATTACTACAGACCTCCAATATCACCAAACTAGAGCAAAAGAAGAACC
CTCAGGCTGTGCTGGATGTCCTAAAGTTCTACGACTCCAACACAGTGAAG
CAGAAATATCTGAGCTTTACTCCTCCTGAGAAAGATGGCTTTCCTTCTGG
AACACCAGCACTGAATGCCAAGGGAACAGAAGCACCCGCAGTAGTGACAG
AGGAGGAGGATGATGATGAAGAGACTGCTCCTCCCGTTATTGCCCCGCGA
CCGGATCATACGAAATCAATTTACACACGGTCTGTAATTGACCCTGTTCC
TGCACCAGTTGGTGATTCACATGTTGATGGTGCTGCCAAGTCTTTAGACA
AACAGAAAAAGAAGACTAAGATGACAGATGAAGAGATTATGGAGAAATTA
AGAACTATCGTGAGCATAGGTGACCCTAAGAAAAAATATACAAGATATGA
AAAAATTGGACAAAGGGCTTCTGGTACAGTTTTCACTGCTACTGACGTTG
CACTGGGACAGGAGGTTGCTATCAAACAAATTAATTTACAGAAACAGCCA
AAGAAGGAACTGATCATTAACGAGATTCTGGTGATGAAAGAATTGAAAAA
TCCCAACATCGTTAACTTTTTGGACAGTTACCTGGTAGGAGATGAATTGT
TTGTGGTCATGGAATACCTTGCTGGGGGGTCACTCACTGATGTGGTAACA
GAAACGTGCATGGATGAAGCACAGATTGCTGCTGTATGCAGAGAGTGTTT
ACAGGCATTGGAGTTTTTACATGCTAATCAAGTGATCCACAGAGACATCA
AAAGTGACAATGTACTTTTGGGAATGGAAGGATCTGTTAAGCTCACTGAC
TTTGGTTTCTGTGCCCAGATCACCCCTGAGCAGAGCAAACGCAGTACCAT
GGTCGGAACGCCATACTGGATGGCACCAGAGGTGGTTACACGGAAAGCTT
ATGGCCCTAAAGTCGACATATGGTCTCTGGGTATCATGGCTATTGAGATG
GTAGAAGGAGAGCCTCCATACCTCAATGAAAATCCCTTGAGGGCCTTGTA
CCTAATAGCAACTAATGGAACCCCAGAACTTCAGAATCCAGAGAAACTTT
CCCCAATATTTCGGGATTTCTTAAATCGATGTTTGGAAATGGATGTGGAA
AAAAGGGGTTCAGCCAAAGAATTATTACAGCATCCTTTCCTGAAACTGGC
CAAACCGTTATCTAGCTTGACACCACTGATCATGGCAGCTAAAGAAGCAA
TGAAGAGTAACCGTTAAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSMSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLK
PLPSVPEEKKPRHKIISIFSGTEKGSKKKEKERPEISPPSDFEHTIHVGF
DAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQK
YLSFTPPEKDGFPSGTPALNAKGTEAPAVVTEEEDDDEETAPPVIAPRPD
HTKSIYTRSVIDPVPAPVGDSHVDGAAKSLDKQKKKTKMTDEEIMEKLRT
IVSIGDPKKKYTRYEKIGQRASGTVFTATDVALGQEVAIKQINLQKQPKK
ELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTET
CMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFG
FCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVE
GEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKR
GSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4499
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA.; Note: This sequence differs from the genomic sequence of PAK2 because there is a G258R mutation in this particular accession.