cDNA Clone NCC

• Plasmid: pGEX6P-1-SLC12A3 (hNCC) 1-139
• Parent Plasmid: pGEX6P1
• Expressed: GST-SLC12A3 (hNCC) 1-139
• Genbank: P55017
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGGCAG
AACTGCCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGGGCGC
TTCACCATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGC
CTATGACAGCAGCCACCCCAGCCACCTGACCCACAGCAGCACCTTCTGCA
TGCGCACCTTTGGCTACAACACGATCGATGTGGTGCCCACATATGAGCAC
TATGCCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCACACT
GGCTGACCTGCACTCCTTCCTCAAGCAGGAAGGCAGACACCTGCATGCCC
TGGCCTTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGTGGAG
GGCGAGGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGCGCTT
CGGCTGGGTCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAELPTTETPGDATLCSGR
FTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEH
YANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVE
GEAGTSSEKNPEEPVRFGWV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4467
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.