cDNA Clone Pias4

• Plasmid: pEBG6P-1 Pias4 W356A
• Parent Plasmid: pEBG6P
• Expressed: GST-Pias4 W356A
• Genbank: BC025159
• Tag: GST
• Species: Mouse

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCGCGGCAGAGCTGGTGGAGGCCAAAAAC
ATGGTGATGAGCTTCCGAGTATCAGACCTGCAGATGCTGCTGGGCTTTGT
AGGCCGGAGCAAGAGTGGGCTGAAGCACGAACTGGTGACCAGGGCCTTGC
AGCTGGTGCAGTTCGACTGTAGCCCAGAGCTTTTCAAGAAGATCAAAGAG
CTGTATGAGACTCGCTATGCCAAGAAGAGTGCAGAGCCCGGCCCACAGGC
ACCACGGCCCCTGGATCCCCTGGCACTACACTCCATGCCCAGGACTCCCC
TGTCAGGGCCCACCGTGGACTACCCCGTGCTGTACGGGAAGTACTTGAAT
GGACTGGGGCGCCTGCCCACCAAGACCCTTAAGCCGGAGGTACGCCTGGT
TAAGCTCCCCTTCTTCAACATGCTGGACGAATTGCTGAAGCCCACTGAAC
TGGTCCCACAGAGTGCAGAGAAGCTGCAAGAGAGCCCATGCATCTTCGCG
CTAACACCTCGGCAGGTGGAGATGATCCGCAACTCCAGGGAGCTGCAGCC
TGGAGTGAAAGCTGTGCAGGTGGTGCTGAGGATCTGTTACTCTGATACCA
GCTGTCCGCAGGAGGACCAATACCCACCCAACATCGCTGTGAAGGTCAAC
CACAGCTACTGCTCAGTGCCGGGCTACTATCCCTCCAACAAGCCTGGTGT
GGAACCTAAGAGGCCCTGCCGCCCCATCAACCTTACCCACCTCATGTATC
TGTCCTCGGCCACCAACCGCATTACTGTCACCTGGGGCAACTATGGCAAG
AGCTACTCTGTGGCTTTGTACCTGGTGCGACAGCTGACCTCCTCAGACCT
GCTACAGAGGTTGAAGACGATTGGGGTGAAGCATCCTGAGCTGTGCAAGG
CTTTGGTCAAGGAGAAGCTTCGCCTAGACCCCGACAGCGAGATCGCCACT
ACCGGAGTGCGAGTGTCCCTCATCTGCCCACTGGTGAAGATGCGCCTCTC
GGTGCCCTGCCGTGCAGAGACCTGCGCACACCTGCAGTGCTTTGATGCTG
TGTTCTACCTGCAAATGAATGAGAAGAAGCCCACCGCGATGTGCCCTGTG
TGTGACAAGCCTGCAGCCTATGACCAGCTCATCATAGATGGGCTGCTGTC
GAAGATCCTGAGTGAGTGTGAGGGCGCGGATGAGATTGAGTTCCTGGCGG
AAGGCTCATGGCGTCCAATCCGTGCAGAGAAGGAGCCAAGCTGCAGCCCA
CAGGGCCCCATCCTGGTGCTAGGCACCTCGGACGCCAATGGGCTCGCACC
AGCCTCCAGCACCCCCGGTATAGGCAGTGGCCTGAGTGGCCCAGGCAGCG
CCGGCAGCGGTGCGGGGGCAGCAGGCAGCCTGGAGAATGGCAAGACGGGA
GCCGACGTGGTGGACCTCACACTGGACAGCTCGTCGTCCTCGGAAGATGA
AGATGAGGACGAGGACGACGATGAGGACGAGGACGAGGGCCCCCGGCCGA
AGCGCCGCTGCCCGTTCCAGAAAGGCCTGGTGCCCGCGTGCTGAGTCGAC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTAAELVEAKN
MVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKE
LYETRYAKKSAEPGPQAPRPLDPLALHSMPRTPLSGPTVDYPVLYGKYLN
GLGRLPTKTLKPEVRLVKLPFFNMLDELLKPTELVPQSAEKLQESPCIFA
LTPRQVEMIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVN
HSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGK
SYSVALYLVRQLTSSDLLQRLKTIGVKHPELCKALVKEKLRLDPDSEIAT
TGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTAMCPV
CDKPAAYDQLIIDGLLSKILSECEGADEIEFLAEGSWRPIRAEKEPSCSP
QGPILVLGTSDANGLAPASSTPGIGSGLSGPGSAGSGAGAAGSLENGKTG
ADVVDLTLDSSSSSEDEDEDEDDDEDEDEGPRPKRRCPFQKGLVPAC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4395
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.