cDNA Clone MEF-DAZAP1-b

• Plasmid: pGEX6P-1-MEF-DAZAP1-b T269D
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEF-DAZAP1-b T269D
• Genbank: NP_005911/NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCGGGAGGAAAAAGATTCAGATCCAGCGAATCACCGACGAGCGGAACCGA
CAGGTGACTTTCACCAAGCGGAAGTTTGGCCTGATGAAGAAGGCGTATGA
GCTGAGCGTGCTATGTGACTGCGAGATCGCACTCATCATCTTCAACCACT
CCAACAAGCTGTTCCAGTACGCCAGCACCGACATGGACAAGGTGCTGCTC
AAGTACACGGAGTACAATGAGCCACACGAGAGCCGCACCAACGCCGACAT
CATCGAGACCCTGAGGAAGAAGGGCTTCAACGGCTGCGACAGTCCCGAGC
CCGACGGGGAGGACTCGCTGGAACAGAGCCCCCTGCTGGAGGACAAGTAC
CGACGCGCCAGCGAGGAGCTCGACGGGCTCTTCCGGCGCTATGGGTCAAC
TGTCCCGGCCCCCAACTTTGCCATGCCTGTCACGGTGCCCGTGTCCAATC
AGAGCTCACTGCAGTTCAGCAATCCCAGCGGCTCCCTGGTCACCCCTTCC
CTGGTGACATCATCCCTCACGGACCCGCGGCTCCTGTCCCCCCAGCAGCC
AGCACTACAGAGGAACAGTGTGTCTCCTGGCCTGCCCCAGCGGCCAGCTA
GTGCGGGGGCCATGCTGGGGGGTGACCTGAACAGTGCTAACGGAGCCTGC
CCCAGCCCTGTTGGGCGAGGTTTTGGATTTATTACTTTCGAGGACGAACA
ATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAA
AAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCG
GGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGC
CAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCC
CGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCG
CCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTA
CATCGTGTCCGACCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTC
AGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCT
CCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCGA
TCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCC
CGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAG
TATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGA
CCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGG
GGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAAC
GTGCAAGGGTTCCACCCCTACCGACGCTAGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFGRKKIQIQRITDERNR
QVTFTKRKFGLMKKAYELSVLCDCEIALIIFNHSNKLFQYASTDMDKVLL
KYTEYNEPHESRTNADIIETLRKKGFNGCDSPEPDGEDSLEQSPLLEDKY
RRASEELDGLFRRYGSTVPAPNFAMPVTVPVSNQSSLQFSNPSGSLVTPS
LVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPASAGAMLGGDLNSANGAC
PSPVGRGFGFITFEDEQSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAP
GQPGASQWGSRVVPNAANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGP
PPAGRGAPPPPPPFTSYIVSDPPGGFPPPQGFPQGYGAPPQFSFGYGPPP
PPPDQFAPPGVPPPPADPGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQ
YAGYGQDLSGFGQGFSDPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHN
VQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4338
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag.