cDNA Clone PIK4CB

• Plasmid: pEBG6P-1 PIK4CB delta 316-330
• Parent Plasmid: pEBG6P
• Expressed: GST-PIK4CB delta 316-330
• Genbank: NP_002642
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCTAGATTCTTGGAAGCTCGAAGTCTGGCTGTGGCCATGGGA
GATACAGTAGTGGAGCCTGCCCCCTTGAAGCCAACTTCTGAGCCCACTTC
TGGCCCACCAGGGAATAATGGGGGGTCCCTGCTAAGTGTCATCACGGAGG
GGGTCGGGGAACTATCAGTGATTGACCCTGAGGTGGCCCAGAAGGCCTGC
CAGGAGGTGTTGGAGAAAGTCAAGCTTTTGCATGGAGGCGTGGCAGTCTC
TAGCAGAGGCACCCCACTGGAGTTGGTCAATGGGGATGGTGTGGACAGTG
AGATCCGTTGCCTAGATGATCCACCTGCCCAGATCAGGGAGGAGGAAGAT
GAGATGGGGGCCGCTGTGGCCTCAGGCACAGCCAAAGGAGCAAGAAGACG
GCGGCAGAACAACTCAGCTAAACAGTCTTGGCTGCTGAGGCTGTTTGAGT
CAAAACTGTTTGACATCTCCATGGCCATTTCATACCTGTATAACTCCAAG
GAGCCTGGAGTACAAGCCTACATTGGCAACCGGCTCTTCTGCTTTCGCAA
CGAGGACGTGGACTTCTATCTGCCCCAGTTGCTTAACATGTACATCCACA
TGGATGAGGACGTGGGTGATGCCATTAAGCCCTACATAGTCCACCGTTGC
CGCCAGAGCATTAACTTTTCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTA
TTCTTCAGACATGCACATTTCCACTCAACGACACTCCCGTGGGACCAAGC
TACGGAAGCTGATCCTCTCAGATGAGCTAAAGCCAGCTCACAGGAAGAGG
GAGCTGCCCTCCTTGAGCCCGGCCCCTGATACAGGGCTGTCTCCCTCCAA
AAGGACTCACCAGCGCTCTAAGTCAGATGCCACTGCCAGCATAAGTCTCA
GCAGCAACCTGAAACGAACAGCCAGCAACCCTAAAGTGGAGAATGAGGAT
GAGCCTGTTCGACTGGCTCCTGAGAGAGAATTCATCAAGTCCCTGATGGC
GATCGGCAAGCGGCTGGCCACGCTCCCCACCAAAGAGCAGAAAACACAGA
GGCTGATCTCAGAGCTCTCCCTGCTCAACCATAAGCTCCCTGCCCGAGTC
TGGCTGCCCACTGCTGGCTTTGACCACCACGTGGTCCGTGTACCCCACAC
ACAGGCTGTTGTCCTCAACTCCAAGGACAAGGCTCCCTACCTGATTTATG
TGGAAGTCCTTGAATGTGAAAACTTTGACACCACCAGTGTCCCTGCCCGG
ATCCCCGAGAACCGAATTCGGAGTACGAGGTCCGTAGAAAACTTGCCCGA
ATGTGGTATTACCCATGAGCAGCGAGCTGGCAGCTTCAGCACTGTGCCCA
ACTATGACAACGATGATGAGGCCTGGTCGGTGGATGACATAGGCGAGCTG
CAAGTGGAGCTCCCCGAAGTGCATACCAACAGCTGTGACAACATCTCCCA
GTTCTCTGTGGACAGCATCACCAGCCAGGAGAGCAAGGAGCCTGTGTTCA
TTGCAGCAGGGGACATCCGCCGGCGCCTTTCGGAACAGCTGGCTCATACC
CCGACAGCCTTCAAACGAGACCCAGAAGATCCTTCTGCAGTTGCTCTCAA
AGAGCCCTGGCAGGAGAAAGTACGGCGGATCAGAGAGGGCTCCCCCTACG
GCCATCTCCCCAATTGGCGGCTCCTGTCAGTCATTGTCAAGTGTGGGGAT
GACCTTCGGCAAGAGCTTCTGGCCTTTCAGGTGTTGAAGCAACTGCAGTC
CATTTGGGAACAGGAGCGAGTGCCCCTTTGGATCAAGCCATACAAGATTC
TTGTGATTTCGGCTGATAGTGGCATGATTGAACCAGTGGTCAATGCTGTG
TCCATCCATCAGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTT
CCTACAGGAGCACGGCAGTTACACCACTGAGGCATTCCTCAGTGCACAGC
GCAATTTTGTGCAAAGTTGTGCTGGGTACTGCTTGGTCTGCTACCTGCTG
CAAGTCAAGGACAGACACAATGGGAATATCCTTTTGGACGCAGAAGGCCA
CATCATCCACATCGACTTTGGCTTCATCCTCTCCAGCTCACCCCGAAATC
TGGGCTTTGAGACGTCAGCCTTTAAGCTGACCACAGAGTTTGTGGATGTG
ATGGGCGGCCTGGATGGCGACATGTTCAACTACTATAAGATGCTGATGCT
GCAAGGGCTGATTGCCGCTCGGAAACACATGGACAAGGTGGTGCAGATCG
TGGAGATCATGCAGCAAGGTTCTCAGCTTCCTTGCTTCCATGGCTCCAGC
ACCATTCGAAACCTCAAAGAGAGGTTCCACATGAGCATGACTGAGGAGCA
GCTGCAGCTGCTGGTGGAGCAGATGGTGGATGGCAGTATGCGGTCTATCA
CCACCAAACTCTATGACGGCTTCCAGTACCTCACCAACGGCATCATGTGA
GCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSRFLEARSLAVAMG
DTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKAC
QEVLEKVKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEED
EMGAAVASGTAKGARRRRQNNSAKQSWLLRLFESKLFDISMAISYLYNSK
EPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRC
RQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKR
ELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENED
EPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARV
WLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPAR
IPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGEL
QVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHT
PTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGD
DLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAV
SIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLL
QVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDV
MGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSS
TIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST. Compare with protein sequence with AAH40300.