cDNA Clone PIK4CB

• Plasmid: pGEX6P-1-PIK4CB delta 316-330
• Parent Plasmid: pGEX6P1
• Expressed: GST-PIK4CB delta 316-330
• Genbank: NP_002642
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCTAGATTCT
TGGAAGCTCGAAGTCTGGCTGTGGCCATGGGAGATACAGTAGTGGAGCCT
GCCCCCTTGAAGCCAACTTCTGAGCCCACTTCTGGCCCACCAGGGAATAA
TGGGGGGTCCCTGCTAAGTGTCATCACGGAGGGGGTCGGGGAACTATCAG
TGATTGACCCTGAGGTGGCCCAGAAGGCCTGCCAGGAGGTGTTGGAGAAA
GTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGCACCCCACT
GGAGTTGGTCAATGGGGATGGTGTGGACAGTGAGATCCGTTGCCTAGATG
ATCCACCTGCCCAGATCAGGGAGGAGGAAGATGAGATGGGGGCCGCTGTG
GCCTCAGGCACAGCCAAAGGAGCAAGAAGACGGCGGCAGAACAACTCAGC
TAAACAGTCTTGGCTGCTGAGGCTGTTTGAGTCAAAACTGTTTGACATCT
CCATGGCCATTTCATACCTGTATAACTCCAAGGAGCCTGGAGTACAAGCC
TACATTGGCAACCGGCTCTTCTGCTTTCGCAACGAGGACGTGGACTTCTA
TCTGCCCCAGTTGCTTAACATGTACATCCACATGGATGAGGACGTGGGTG
ATGCCATTAAGCCCTACATAGTCCACCGTTGCCGCCAGAGCATTAACTTT
TCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTATTCTTCAGACATGCACAT
TTCCACTCAACGACACTCCCGTGGGACCAAGCTACGGAAGCTGATCCTCT
CAGATGAGCTAAAGCCAGCTCACAGGAAGAGGGAGCTGCCCTCCTTGAGC
CCGGCCCCTGATACAGGGCTGTCTCCCTCCAAAAGGACTCACCAGCGCTC
TAAGTCAGATGCCACTGCCAGCATAAGTCTCAGCAGCAACCTGAAACGAA
CAGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGCCTGTTCGACTGGCT
CCTGAGAGAGAATTCATCAAGTCCCTGATGGCGATCGGCAAGCGGCTGGC
CACGCTCCCCACCAAAGAGCAGAAAACACAGAGGCTGATCTCAGAGCTCT
CCCTGCTCAACCATAAGCTCCCTGCCCGAGTCTGGCTGCCCACTGCTGGC
TTTGACCACCACGTGGTCCGTGTACCCCACACACAGGCTGTTGTCCTCAA
CTCCAAGGACAAGGCTCCCTACCTGATTTATGTGGAAGTCCTTGAATGTG
AAAACTTTGACACCACCAGTGTCCCTGCCCGGATCCCCGAGAACCGAATT
CGGAGTACGAGGTCCGTAGAAAACTTGCCCGAATGTGGTATTACCCATGA
GCAGCGAGCTGGCAGCTTCAGCACTGTGCCCAACTATGACAACGATGATG
AGGCCTGGTCGGTGGATGACATAGGCGAGCTGCAAGTGGAGCTCCCCGAA
GTGCATACCAACAGCTGTGACAACATCTCCCAGTTCTCTGTGGACAGCAT
CACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTGCAGCAGGGGACATCC
GCCGGCGCCTTTCGGAACAGCTGGCTCATACCCCGACAGCCTTCAAACGA
GACCCAGAAGATCCTTCTGCAGTTGCTCTCAAAGAGCCCTGGCAGGAGAA
AGTACGGCGGATCAGAGAGGGCTCCCCCTACGGCCATCTCCCCAATTGGC
GGCTCCTGTCAGTCATTGTCAAGTGTGGGGATGACCTTCGGCAAGAGCTT
CTGGCCTTTCAGGTGTTGAAGCAACTGCAGTCCATTTGGGAACAGGAGCG
AGTGCCCCTTTGGATCAAGCCATACAAGATTCTTGTGATTTCGGCTGATA
GTGGCATGATTGAACCAGTGGTCAATGCTGTGTCCATCCATCAGGTGAAG
AAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAGGAGCACGGCAG
TTACACCACTGAGGCATTCCTCAGTGCACAGCGCAATTTTGTGCAAAGTT
GTGCTGGGTACTGCTTGGTCTGCTACCTGCTGCAAGTCAAGGACAGACAC
AATGGGAATATCCTTTTGGACGCAGAAGGCCACATCATCCACATCGACTT
TGGCTTCATCCTCTCCAGCTCACCCCGAAATCTGGGCTTTGAGACGTCAG
CCTTTAAGCTGACCACAGAGTTTGTGGATGTGATGGGCGGCCTGGATGGC
GACATGTTCAACTACTATAAGATGCTGATGCTGCAAGGGCTGATTGCCGC
TCGGAAACACATGGACAAGGTGGTGCAGATCGTGGAGATCATGCAGCAAG
GTTCTCAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGAAACCTCAAA
GAGAGGTTCCACATGAGCATGACTGAGGAGCAGCTGCAGCTGCTGGTGGA
GCAGATGGTGGATGGCAGTATGCGGTCTATCACCACCAAACTCTATGACG
GCTTCCAGTACCTCACCAACGGCATCATGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSRFLEARSLAVAMGDTVVEP
APLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEK
VKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAV
ASGTAKGARRRRQNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQA
YIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINF
SLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLS
PAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEPVRLA
PEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAG
FDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRI
RSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPE
VHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKR
DPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQEL
LAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVK
KQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRH
NGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDG
DMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLK
ERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4273
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST. Compare with protein sequence with AAH40300.