cDNA Clone MEF-DAZAP1-b

• Plasmid: pCMV5-HA MEF-DAZAP T315D
• Parent Plasmid: pCMV5
• Expressed: HA-MEF-DAZAP T315D
• Genbank: NP_005911/NM_018959
• Tag: HA
• Species: Human

Sequence of Insert

ATGTACCCATACGATGTGCCAGATTACGCCGGGAGGAAAAAGATTCAGAT
CCAGCGAATCACCGACGAGCGGAACCGACAGGTGACTTTCACCAAGCGGA
AGTTTGGCCTGATGAAGAAGGCGTATGAGCTGAGCGTGCTATGTGACTGC
GAGATCGCACTCATCATCTTCAACCACTCCAACAAGCTGTTCCAGTACGC
CAGCACCGACATGGACAAGGTGCTGCTCAAGTACACGGAGTACAATGAGC
CACACGAGAGCCGCACCAACGCCGACATCATCGAGACCCTGAGGAAGAAG
GGCTTCAACGGCTGCGACAGTCCCGAGCCCGACGGGGAGGACTCGCTGGA
ACAGAGCCCCCTGCTGGAGGACAAGTACCGACGCGCCAGCGAGGAGCTCG
ACGGGCTCTTCCGGCGCTATGGGTCAACTGTCCCGGCCCCCAACTTTGCC
ATGCCTGTCACGGTGCCCGTGTCCAATCAGAGCTCACTGCAGTTCAGCAA
TCCCAGCGGCTCCCTGGTCACCCCTTCCCTGGTGACATCATCCCTCACGG
ACCCGCGGCTCCTGTCCCCCCAGCAGCCAGCACTACAGAGGAACAGTGTG
TCTCCTGGCCTGCCCCAGCGGCCAGCTAGTGCGGGGGCCATGCTGGGGGG
TGACCTGAACAGTGCTAACGGAGCCTGCCCCAGCCCTGTTGGGCGAGGTT
TTGGATTTATTACTTTCGAGGACGAACAATCAGTGGACCAGGCTGTCAAC
ATGCATTTTCACGACATCATGGGCAAAAAAGTGGAAGTTAAACGAGCTGA
GCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGCCAGGTGCCAGCCAGT
GGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGCCAGCCC
CCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGC
AGGACAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCC
CCCCGCCACCCCCACCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGA
GGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTACGGTGCCCCGCCACA
GTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGCCAGATCAGTTTGCCC
CTCCGGGGGTTCCTCCTCCACCAGCCGATCCCGGGGCAGCACCTCTGGCT
TTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGAC
AGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACT
TGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCC
TACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCCCCCCGCCGGCGGCAG
CGGCTTTGGACGAGGGCAGAACCACAACGTGCAAGGGTTCCACCCCTACC
GACGCTAGGTCGAC

Amino Acid Sequence

MYPYDVPDYAGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDC
EIALIIFNHSNKLFQYASTDMDKVLLKYTEYNEPHESRTNADIIETLRKK
GFNGCDSPEPDGEDSLEQSPLLEDKYRRASEELDGLFRRYGSTVPAPNFA
MPVTVPVSNQSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNSV
SPGLPQRPASAGAMLGGDLNSANGACPSPVGRGFGFITFEDEQSVDQAVN
MHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANGWAGQP
PPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIVSTPPG
GFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPADPGAAPLA
FPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPSQQPPS
YGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4260
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA tag.