cDNA Clone Pias4

• Plasmid: pGEX6P-1-Pias4
• Parent Plasmid: pGEX6P1
• Expressed: GST-Pias4
• Genbank: BC025159
• Tag: GST
• Species: Mouse

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCGCGGCAGAGCTGGTGGAGGCCAAAAACATGGTGATGAGCTTCCGAGTA
TCAGACCTGCAGATGCTGCTGGGCTTTGTAGGCCGGAGCAAGAGTGGGCT
GAAGCACGAACTGGTGACCAGGGCCTTGCAGCTGGTGCAGTTCGACTGTA
GCCCAGAGCTTTTCAAGAAGATCAAAGAGCTGTATGAGACTCGCTATGCC
AAGAAGAGTGCAGAGCCCGGCCCACAGGCACCACGGCCCCTGGATCCCCT
GGCACTACACTCCATGCCCAGGACTCCCCTGTCAGGGCCCACCGTGGACT
ACCCCGTGCTGTACGGGAAGTACTTGAATGGACTGGGGCGCCTGCCCACC
AAGACCCTTAAGCCGGAGGTACGCCTGGTTAAGCTCCCCTTCTTCAACAT
GCTGGACGAATTGCTGAAGCCCACTGAACTGGTCCCACAGAGTGCAGAGA
AGCTGCAAGAGAGCCCATGCATCTTCGCGCTAACACCTCGGCAGGTGGAG
ATGATCCGCAACTCCAGGGAGCTGCAGCCTGGAGTGAAAGCTGTGCAGGT
GGTGCTGAGGATCTGTTACTCTGATACCAGCTGTCCGCAGGAGGACCAAT
ACCCACCCAACATCGCTGTGAAGGTCAACCACAGCTACTGCTCAGTGCCG
GGCTACTATCCCTCCAACAAGCCTGGTGTGGAACCTAAGAGGCCCTGCCG
CCCCATCAACCTTACCCACCTCATGTATCTGTCCTCGGCCACCAACCGCA
TTACTGTCACCTGGGGCAACTATGGCAAGAGCTACTCTGTGGCTTTGTAC
CTGGTGCGACAGCTGACCTCCTCAGACCTGCTACAGAGGTTGAAGACGAT
TGGGGTGAAGCATCCTGAGCTGTGCAAGGCTTTGGTCAAGGAGAAGCTTC
GCCTAGACCCCGACAGCGAGATCGCCACTACCGGAGTGCGAGTGTCCCTC
ATCTGCCCACTGGTGAAGATGCGCCTCTCGGTGCCCTGCCGTGCAGAGAC
CTGCGCACACCTGCAGTGCTTTGATGCTGTGTTCTACCTGCAAATGAATG
AGAAGAAGCCCACCTGGATGTGCCCTGTGTGTGACAAGCCTGCAGCCTAT
GACCAGCTCATCATAGATGGGCTGCTGTCGAAGATCCTGAGTGAGTGTGA
GGGCGCGGATGAGATTGAGTTCCTGGCGGAAGGCTCATGGCGTCCAATCC
GTGCAGAGAAGGAGCCAAGCTGCAGCCCACAGGGCCCCATCCTGGTGCTA
GGCACCTCGGACGCCAATGGGCTCGCACCAGCCTCCAGCACCCCCGGTAT
AGGCAGTGGCCTGAGTGGCCCAGGCAGCGCCGGCAGCGGTGCGGGGGCAG
CAGGCAGCCTGGAGAATGGCAAGACGGGAGCCGACGTGGTGGACCTCACA
CTGGACAGCTCGTCGTCCTCGGAAGATGAAGATGAGGACGAGGACGACGA
TGAGGACGAGGACGAGGGCCCCCGGCCGAAGCGCCGCTGCCCGTTCCAGA
AAGGCCTGGTGCCCGCGTGCTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFAAELVEAKNMVMSFRV
SDLQMLLGFVGRSKSGLKHELVTRALQLVQFDCSPELFKKIKELYETRYA
KKSAEPGPQAPRPLDPLALHSMPRTPLSGPTVDYPVLYGKYLNGLGRLPT
KTLKPEVRLVKLPFFNMLDELLKPTELVPQSAEKLQESPCIFALTPRQVE
MIRNSRELQPGVKAVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVP
GYYPSNKPGVEPKRPCRPINLTHLMYLSSATNRITVTWGNYGKSYSVALY
LVRQLTSSDLLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSL
ICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAAY
DQLIIDGLLSKILSECEGADEIEFLAEGSWRPIRAEKEPSCSPQGPILVL
GTSDANGLAPASSTPGIGSGLSGPGSAGSGAGAAGSLENGKTGADVVDLT
LDSSSSSEDEDEDEDDDEDEDEGPRPKRRCPFQKGLVPAC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4252
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.