cDNA Clone UBE2Q1

• Plasmid: pGEX6P1 UBE2Q1
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C UBE2Q1
• Genbank: NP_060052
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAG
CTGGGGGGCCAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGGGGGG
CCCGGGGCCGGGGCCCTGCCTGAGGCGAGAGCTGAAGCTGCTCGAGTCCA
TCTTCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCTGGAC
GAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGGGCCGGGGCGGG
GGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGGGATC
CTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCCCC
ATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAG
GCTGGTGGACATAAAGAAAGGGAATACTCTGCTATTGCAGCATCTGAAGA
GGATCATCTCCGACCTGTGTAAACTCTATAACCTCCCTCAGCATCCAGAT
GTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGCACACAGGAAGA
CGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGACT
TAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAAATCT
GAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTAA
AAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCGGTGC
AGGCCACTGACCGGCTGATGAAGGAGCTCAGGGATATATACCGATCACAG
AGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAATGACAGTCTGTA
TGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCACA
ACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTT
AACTTTTCCTTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCAGGGT
TGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCCATCT
GCATGGAACTTCTCACCAAACAGGGCTGGAGCAGTGCCTACTCCATAGAG
TCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGGAAAGCACGAGT
GCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAGCAGT
CCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCC
CCAAAAGAAGACGGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFQQPQPQGQQQPGPGQQ
LGGQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFRIASACLD
ELSCEFLLAGAGGAGAGAAPGPHLPPRGSVPGDPVRIHCNITESYPAVPP
IWSVESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLYNLPQHPD
VEMLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKS
EDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQ
SFKGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILL
NFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIE
SVIMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTP
PKEDG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4213
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.
• Low Temp: No