cDNA Clone UBE2A

• Plasmid: pGEX6P-3-UBE2A
• Parent Plasmid: pGEX6P3
• Expressed: GST-UBE2A
• Genbank: NP_003327
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATT
CCCGGGTCGACTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAG
AGGTTGCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAA
CAACATAATGGTGTGGAACGCGGTCATTTTCGGGCCTGAAGGGACCCCGT
TTGAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCCA
AATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGT
CTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTC
CAACCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGAT
GAACCCAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCA
GGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAA
GCTGGCGTGATTGTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDSTPARRRLMRDFK
RLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYP
NKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLD
EPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4203
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.