cDNA Clone PAK6

• Plasmid: pGEX6P-1-PAK6
• Parent Plasmid: pGEX6P1
• Expressed: GST-PAK6
• Genbank: Q9NQU5
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCTTCCGCAAGAAAAAGAAGAAACGCCCTGAGATCTCAGCGCCACAGAAC
TTCCAGCACCGTGTCCACACCTCCTTCGACCCCAAAGAAGGCAAGTTTGT
GGGCCTCCCCCCACAATGGCAGAACATCCTGGACACACTGCGGCGCCCCA
AGCCCGTGGTGGACCCTTCGCGAATCACACGGGTGCAGCTCCAGCCCATG
AAGACAGTGGTGCGGGGCAGCGCGATGCCTGTGGATGGCTACATCTCGGG
GCTGCTCAACGACATCCAGAAGTTGTCAGTCATCAGCTCCAACACCCTGC
GTGGCCGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCTG
GGGGATGAGCACTGGGCCACCGACCCAGACATGTACCTCCAGAGCCCCCA
GTCTGAGCGCACTGACCCCCACGGCCTCTACCTCAGCTGCAACGGGGGCA
CACCAGCAGGCCACAAGCAGATGCCGTGGCCCGAGCCACAGAGCCCACGG
GTCCTGCCCAATGGGCTGGCTGCAAAGGCACAGTCCCTGGGCCCCGCCGA
GTTTCAGGGTGCCTCGCAGCGCTGTCTGCAGCTGGGTGCCTGCCTGCAGA
GCTCCCCACCAGGAGCCTCGCCCCCCACGGGCACCAATAGGCATGGAATG
AAGGCTGCCAAGCATGGCTCTGAGGAGGCCCGGCCACAGTCCTGCCTGGT
GGGCTCAGCCACAGGCAGGCCAGGTGGGGAAGGCAGCCCTAGCCCTAAGA
CCCGGGAGAGCAGCCTGAAGCGCAGGCTATTCCGAAGCATGTTCCTGTCC
ACTGCTGCCACAGCCCCTCCAAGCAGCAGCAAGCCAGGCCCTCCACCACA
GAGCAAGCCCAACTCCTCTTTCCGACCGCCGCAGAAAGACAACCCCCCAA
GCCTGGTGGCCAAGGCCCAGTCCTTGCCCTCGGACCAGCCGGTGGGGACC
TTCAGCCCTCTGACCACTTCGGATACCAGCAGCCCCCAGAAGTCCCTCCG
CACAGCCCCGGCCACAGGCCAGCTTCCAGGCCGGTCTTCCCCAGCGGGAT
CCCCCCGCACCTGGCACGCCCAGATCAGCACCAGCAACCTGTACCTGCCC
CAGGACCCCACGGTTGCCAAGGGTGCCCTGGCTGGTGAGGACACAGGTGT
TGTGACACATGAGCAGTTCAAGGCTGCGCTCAGGATGGTGGTGGACCAGG
GTGACCCCCGGCTGCTGCTGGACAGCTACGTGAAGATTGGCGAGGGCTCC
ACCGGCATCGTCTGCTTGGCCCGGGAGAAGCACTCGGGCCGCCAGGTGGC
CGTCAAGATGATGGACCTCAGGAAGCAGCAGCGCAGGGAGCTGCTCTTCA
ACGAGGTGGTGATCATGCGGGACTACCAGCACTTCAACGTGGTGGAGATG
TACAAGAGCTACCTGGTGGGCGAGGAGCTGTGGGTGCTCATGGAGTTCCT
GCAGGGAGGAGCCCTCACAGACATCGTCTCCCAAGTCAGGCTGAATGAGG
AGCAGATTGCCACTGTGTGTGAGGCTGTGCTGCAGGCCCTGGCCTACCTG
CATGCTCAGGGTGTCATCCACCGGGACATCAAGAGTGACTCCATCCTGCT
GACCCTCGATGGCAGGGTGAAGCTCTCGGACTTCGGATTCTGTGCTCAGA
TCAGCAAAGACGTCCCTAAGAGGAAGTCCCTGGTGGGAACCCCCTACTGG
ATGGCTCCTGAAGTGATCTCCAGGTCTTTGTATGCCACTGAGGTGGATAT
CTGGTCTCTGGGCATCATGGTGATTGAGATGGTAGATGGGGAGCCACCGT
ACTTCAGTGACTCCCCAGTGCAAGCCATGAAGAGGCTCCGGGACAGCCCC
CCACCCAAGCTGAAAAACTCTCACAAGGTCTCCCCAGTGCTGCGAGACTT
CCTGGAGCGGATGCTGGTGCGGGACCCCCAAGAGAGAGCCACAGCCCAGG
AGCTCCTAGACCACCCCTTCCTGCTGCAGACAGGGCTACCTGAGTGCCTG
GTGCCCCTGATCCAGCTCTACCGAAAGCAGACCTCCACCTGCTGAGCGGC
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFFRKKKKKRPEISAPQN
FQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPM
KTVVRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLL
GDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPR
VLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGM
KAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLS
TAATAPPSSSKPGPPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGT
FSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLP
QDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGS
TGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEM
YKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYL
HAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYW
MAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSP
PPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECL
VPLIQLYRKQTSTC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4190
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.