cDNA Clone PRP4K

• Plasmid: pEBG6P-1 PRP4K catalytic domain
• Parent Plasmid: pEBG6P
• Expressed: GST-PRP4K catalytic domain
• Genbank: AAH34969
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCAAACGTTACAATGTGTATGGCTACACTGGGCAAGGTGTA
TTCAGTAATGTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGT
GGCTGTAAAGATCATCAGAAACAATGAGCTCATGCAAAAGACTGGTTTAA
AAGAATTAGAGTTCTTGAAAAAACTTAATGATGCTGATCCTGATGACAAA
TTTCATTGTCTGAGACTCTTAAGGCACTTCTATCACAAGCAGCATCTTTG
TCTGGTATTCGAGCCTCTCAGCATGAACTTACGAGAGGTGTTAAAAAAAT
ATGGTAAAGATGTTGGTCTTCATATTAAAGCTGTAAGATCCTATAGTCAG
CAGTTGTTCCTGGCATTGAAACTCCTTAAAAGATGCAATATCCTACATGC
AGATATCAAGCCAGACAATATCCTGGTTAATGAATCCAAAACTATTTTAA
AGCTTTGCGATTTTGGGTCGGCTTCACATGTTGCGGATAATGACATAACA
CCTTATCTTTTCAGTAGATTTTATCGTGCTCCTGAAATCATTATAGGTAA
AAGCTATGACTATGGTATAGATATGTGGTCTGTAGGTTGCACCTTATACG
AACTCTATACTGGAAAAATTTTATTCCCTGGCAAAACCAATAACCATATG
CTGAAGCTTGCAATGGATCTCAAAGGAAAGATGCCAAATAAGATGATTCG
AAAAGGTGTGTTCAAAGATCAGCATTTTGATCAAAATCTCAACTTCATGT
ACATAGAAGTTGATAAAGTAACAGAGAGGGAGAAAGTTACTGTTATGAGC
ACCATTAATCCAACTAAGGACCTGTTGGCTGACTTGATTGGGTGCCAGAG
ACTTCCTGAAGACCAACGTAAGAAAGTACACCAGCTAAAGGACTTGTTGG
ACCAGATTCTGATGTTGGACCCAGCTAAACGAATTAGCATCAACCAGGCC
CTACAGCACGCCTTCATCCAGGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSKRYNVYGYTGQGV
FSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDK
FHCLRLLRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQ
QLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDIT
PYLFSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHM
LKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMS
TINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQA
LQHAFIQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4147
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.