cDNA Clone PRP4K

• Plasmid: pGEX6P-1-PRP4K catalytic domain
• Parent Plasmid: pGEX6P1
• Expressed: GST-PRP4K catalytic domain
• Genbank: AAH34969
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCAAACGTT
ACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAATGTTGTACGA
GCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAAAGATCATCAG
AAACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGA
AAAAACTTAATGATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTC
TTAAGGCACTTCTATCACAAGCAGCATCTTTGTCTGGTATTCGAGCCTCT
CAGCATGAACTTACGAGAGGTGTTAAAAAAATATGGTAAAGATGTTGGTC
TTCATATTAAAGCTGTAAGATCCTATAGTCAGCAGTTGTTCCTGGCATTG
AAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAAGCCAGACAA
TATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGT
CGGCTTCACATGTTGCGGATAATGACATAACACCTTATCTTTTCAGTAGA
TTTTATCGTGCTCCTGAAATCATTATAGGTAAAAGCTATGACTATGGTAT
AGATATGTGGTCTGTAGGTTGCACCTTATACGAACTCTATACTGGAAAAA
TTTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTTGCAATGGAT
CTCAAAGGAAAGATGCCAAATAAGATGATTCGAAAAGGTGTGTTCAAAGA
TCAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAGTTGATAAAG
TAACAGAGAGGGAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAG
GACCTGTTGGCTGACTTGATTGGGTGCCAGAGACTTCCTGAAGACCAACG
TAAGAAAGTACACCAGCTAAAGGACTTGTTGGACCAGATTCTGATGTTGG
ACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCACGCCTTCATC
CAGGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSKRYNVYGYTGQGVFSNVVR
ARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRL
LRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLAL
KLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPYLFSR
FYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMD
LKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTK
DLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFI
QE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4146
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.