cDNA Clone PRP4K

• Plasmid: pCMV5-HA PRP4K
• Parent Plasmid: pCMV5
• Expressed: HA-PRP4K
• Genbank: AAH34969
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCAGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGC
CGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTA
ATTCTGAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAG
TCTCAAAATAAGCACAGTCGTCACAAAAAAAAGAAGCATAAACACAGAAG
TAAACATAAGAAACATAAACATTCCTCAGAAGAAGACAAGGATAAAAAAC
ATAAACATAAGCATAAACATAAGAAACACAAAAGAAAAGAGGTTATTGAT
GCTTCTGATAAAGAGGGTATGTCTCCAGCAAAAAGAACTAAACTTGATGA
TTTAGCTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAAGGCCG
AACTTGATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTC
ATTTTGCAAGGTTATGAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGA
AAAGGCAAGAAATGGAAATAGGTCTAGTACTAGATCTTCAAGTACAAAGG
GGAAACTTGAACTTGTGGACAATAAAATTACTACAAAGAAACGAAGTAAA
AGCAGATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAAAGTAA
GGGGGGTATTGAAATCGTTAAAGAGAAAACAACTAGGAGCAAGTCAAAGG
AGAGGAAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAGATCAA
GCAAGGAAATCAAAATCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAAT
TGGTAAGGCCAGATCTCCTACTGATGATAAGGTTAAAATTGAAGATAAAA
GTAAATCAAAAGATAGGAAAAAATCCCCAATTATAAATGAAAGTAGAAGT
CGCGATCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGTAAATC
CAAAGACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAA
CTGATAAAGAAAAGAAGCCAATTAAATCTCCCTCTAAAGATGCTTCATCT
GGGAAAGAAAATAGGTCACCCAGCAGAAGACCTGGTCGTAGTCCTAAAAG
AAGAAGTTTGTCTCCAAAACCACGTGATAAATCAAGAAGAAGCAGGTCTC
CACTTTTGAATGATAGAAGATCTAAGCAGAGCAAATCCCCCTCGCGGACA
CTGTCTCCTGGGAGAAGAGCCAAGAGCCGATCCTTAGAAAGAAAACGACG
AGAACCAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATG
ATATCCTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGA
TGGTCTCCAACCCGAAGAAGAAGTAGATCTCCCATTAGAAGGAGGTCTCG
TTCCCCACTCAGACGTAGCAGGTCTCCAAGAAGAAGAAGCAGATCTACTC
GGAGAAGGGACAGAGGTCGGAGGAGCAGATCACGCTTGCGAAGGCGGTCT
CGATCACGCGGTGGTCGTAGACGAAGGAGCAGAAGCAAAGTAAAGGAAGA
TAAATTTAAAGGAAGTCTTTCTGAAGGAATGAAAGTTGAGCAGGAATCTT
CGTCTGATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAA
GCCCTAATAGAACAGAGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATA
TAAATACCTTGCTGAAGATAGCAACATGTCTGTGCCATCTGAACCAAGCA
GCCCCCAGAGCAGTACGAGAACACGATCACCATCTCCAGATGACATTCTG
GAGCGAGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTTGATAC
ATTTGAGGCCTCAGTGAAAGCCAAGCATAATCTAATGACAGTTGAACAGA
ATAATGGTTCATCTCAGAAGAAGTTGTTGGCACCTGATATGTTTACAGAA
TCTGATGATATGTTTGCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGC
TGGCATTGGAAAAGATTTCAAAGAGAATCCCAACCTCAGAGATAACTGGA
CCGATGCAGAAGGCTATTATCGTGTGAACATAGGTGAAGTCCTAGATAAA
CGTTACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAATGTTGT
ACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAAAGATCA
TCAGAAACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTC
TTGAAAAAACTTAATGATGCTGATCCTGATGACAAATTTCATTGTCTGAG
ACTCTTAAGGCACTTCTATCACAAGCAGCATCTTTGTCTGGTATTCGAGC
CTCTCAGCATGAACTTACGAGAGGTGTTAAAAAAATATGGTAAAGATGTT
GGTCTTCATATTAAAGCTGTAAGATCCTATAGTCAGCAGTTGTTCCTGGC
ATTGAAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAAGCCAG
ACAATATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTT
GGGTCGGCTTCACATGTTGCGGATAATGACATAACACCTTATCTTTTCAG
TAGATTTTATCGTGCTCCTGAAATCATTATAGGTAAAAGCTATGACTATG
GTATAGATATGTGGTCTGTAGGTTGCACCTTATACGAACTCTATACTGGA
AAAATTTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTTGCAAT
GGATCTCAAAGGAAAGATGCCAAATAAGATGATTCGAAAAGGTGTGTTCA
AAGATCAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAGTTGAT
AAAGTAACAGAGAGGGAGAAAGTTACTGTTATGAGCACCATTAATCCAAC
TAAGGACCTGTTGGCTGACTTGATTGGGTGCCAGAGACTTCCTGAAGACC
AACGTAAGAAAGTACACCAGCTAAAGGACTTGTTGGACCAGATTCTGATG
TTGGACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCACGCCTT
CATCCAGGAAAAAATTTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAAAAETQSLREQPEMEDANSEKSINEENGEVSEDQSQNKHS
RHKKKKHKHRSKHKKHKHSSEEDKDKKHKHKHKHKKHKRKEVIDASDKEG
MSPAKRTKLDDLALLEDLEKQRALIKAELDNELMEGKVQSGMGLILQGYE
SGSEEEGEIHEKARNGNRSSTRSSSTKGKLELVDNKITTKKRSKSRSKER
TRHRSDKKKSKGGIEIVKEKTTRSKSKERKKSKSPSKRSKSQDQARKSKS
PTLRRRSQEKIGKARSPTDDKVKIEDKSKSKDRKKSPIINESRSRDRGKK
SRSPVDLRGKSKDRRSRSKERKSKRSETDKEKKPIKSPSKDASSGKENRS
PSRRPGRSPKRRSLSPKPRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRR
AKSRSLERKRREPERRRLSSPRTRPRDDILSRRERSKDASPINRWSPTRR
RSRSPIRRRSRSPLRRSRSPRRRSRSTRRRDRGRRSRSRLRRRSRSRGGR
RRRSRSKVKEDKFKGSLSEGMKVEQESSSDDNLEDFDVEEEDEEALIEQR
RIQRQAIVQKYKYLAEDSNMSVPSEPSSPQSSTRTRSPSPDDILERVAAD
VKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAPDMFTESDDMFA
AYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYG
YTGQGVFSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLND
ADPDDKFHCLRLLRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKA
VRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHV
ADNDITPYLFSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPG
KTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTERE
KVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKR
ISINQALQHAFIQEKI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4144
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal HA.