cDNA Clone PRP4K

• Plasmid: pGEX6P-1-PRP4K
• Parent Plasmid: pGEX6P1
• Expressed: GST-PRP4K
• Genbank: AAH34969
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGCCGCCG
CGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTAATTCT
GAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCA
AAATAAGCACAGTCGTCACAAAAAAAAGAAGCATAAACACAGAAGTAAAC
ATAAGAAACATAAACATTCCTCAGAAGAAGACAAGGATAAAAAACATAAA
CATAAGCATAAACATAAGAAACACAAAAGAAAAGAGGTTATTGATGCTTC
TGATAAAGAGGGTATGTCTCCAGCAAAAAGAACTAAACTTGATGATTTAG
CTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAAGGCCGAACTT
GATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTCATTTT
GCAAGGTTATGAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGAAAAGG
CAAGAAATGGAAATAGGTCTAGTACTAGATCTTCAAGTACAAAGGGGAAA
CTTGAACTTGTGGACAATAAAATTACTACAAAGAAACGAAGTAAAAGCAG
ATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAAAGTAAGGGGG
GTATTGAAATCGTTAAAGAGAAAACAACTAGGAGCAAGTCAAAGGAGAGG
AAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAGATCAAGCAAG
GAAATCAAAATCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAATTGGTA
AGGCCAGATCTCCTACTGATGATAAGGTTAAAATTGAAGATAAAAGTAAA
TCAAAAGATAGGAAAAAATCCCCAATTATAAATGAAAGTAGAAGTCGCGA
TCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGTAAATCCAAAG
ACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAACTGAT
AAAGAAAAGAAGCCAATTAAATCTCCCTCTAAAGATGCTTCATCTGGGAA
AGAAAATAGGTCACCCAGCAGAAGACCTGGTCGTAGTCCTAAAAGAAGAA
GTTTGTCTCCAAAACCACGTGATAAATCAAGAAGAAGCAGGTCTCCACTT
TTGAATGATAGAAGATCTAAGCAGAGCAAATCCCCCTCGCGGACACTGTC
TCCTGGGAGAAGAGCCAAGAGCCGATCCTTAGAAAGAAAACGACGAGAAC
CAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATGATATC
CTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGATGGTC
TCCAACCCGAAGAAGAAGTAGATCTCCCATTAGAAGGAGGTCTCGTTCCC
CACTCAGACGTAGCAGGTCTCCAAGAAGAAGAAGCAGATCTACTCGGAGA
AGGGACAGAGGTCGGAGGAGCAGATCACGCTTGCGAAGGCGGTCTCGATC
ACGCGGTGGTCGTAGACGAAGGAGCAGAAGCAAAGTAAAGGAAGATAAAT
TTAAAGGAAGTCTTTCTGAAGGAATGAAAGTTGAGCAGGAATCTTCGTCT
GATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAAGCCCT
AATAGAACAGAGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATATAAAT
ACCTTGCTGAAGATAGCAACATGTCTGTGCCATCTGAACCAAGCAGCCCC
CAGAGCAGTACGAGAACACGATCACCATCTCCAGATGACATTCTGGAGCG
AGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTTGATACATTTG
AGGCCTCAGTGAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAAT
GGTTCATCTCAGAAGAAGTTGTTGGCACCTGATATGTTTACAGAATCTGA
TGATATGTTTGCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGCTGGCA
TTGGAAAAGATTTCAAAGAGAATCCCAACCTCAGAGATAACTGGACCGAT
GCAGAAGGCTATTATCGTGTGAACATAGGTGAAGTCCTAGATAAACGTTA
CAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAATGTTGTACGAG
CCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAAAGATCATCAGA
AACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGAA
AAAACTTAATGATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCT
TAAGGCACTTCTATCACAAGCAGCATCTTTGTCTGGTATTCGAGCCTCTC
AGCATGAACTTACGAGAGGTGTTAAAAAAATATGGTAAAGATGTTGGTCT
TCATATTAAAGCTGTAAGATCCTATAGTCAGCAGTTGTTCCTGGCATTGA
AACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAAGCCAGACAAT
ATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGTC
GGCTTCACATGTTGCGGATAATGACATAACACCTTATCTTTTCAGTAGAT
TTTATCGTGCTCCTGAAATCATTATAGGTAAAAGCTATGACTATGGTATA
GATATGTGGTCTGTAGGTTGCACCTTATACGAACTCTATACTGGAAAAAT
TTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTTGCAATGGATC
TCAAAGGAAAGATGCCAAATAAGATGATTCGAAAAGGTGTGTTCAAAGAT
CAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAGTTGATAAAGT
AACAGAGAGGGAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAGG
ACCTGTTGGCTGACTTGATTGGGTGCCAGAGACTTCCTGAAGACCAACGT
AAGAAAGTACACCAGCTAAAGGACTTGTTGGACCAGATTCTGATGTTGGA
CCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCACGCCTTCATCC
AGGAAAAAATTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAETQSLREQPEMEDANS
EKSINEENGEVSEDQSQNKHSRHKKKKHKHRSKHKKHKHSSEEDKDKKHK
HKHKHKKHKRKEVIDASDKEGMSPAKRTKLDDLALLEDLEKQRALIKAEL
DNELMEGKVQSGMGLILQGYESGSEEEGEIHEKARNGNRSSTRSSSTKGK
LELVDNKITTKKRSKSRSKERTRHRSDKKKSKGGIEIVKEKTTRSKSKER
KKSKSPSKRSKSQDQARKSKSPTLRRRSQEKIGKARSPTDDKVKIEDKSK
SKDRKKSPIINESRSRDRGKKSRSPVDLRGKSKDRRSRSKERKSKRSETD
KEKKPIKSPSKDASSGKENRSPSRRPGRSPKRRSLSPKPRDKSRRSRSPL
LNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRLSSPRTRPRDDI
LSRRERSKDASPINRWSPTRRRSRSPIRRRSRSPLRRSRSPRRRSRSTRR
RDRGRRSRSRLRRRSRSRGGRRRRSRSKVKEDKFKGSLSEGMKVEQESSS
DDNLEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSNMSVPSEPSSP
QSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNN
GSSQKKLLAPDMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTD
AEGYYRVNIGEVLDKRYNVYGYTGQGVFSNVVRARDNARANQEVAVKIIR
NNELMQKTGLKELEFLKKLNDADPDDKFHCLRLLRHFYHKQHLCLVFEPL
SMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDN
ILVNESKTILKLCDFGSASHVADNDITPYLFSRFYRAPEIIIGKSYDYGI
DMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKD
QHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQR
KKVHQLKDLLDQILMLDPAKRISINQALQHAFIQEKI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4138
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.