cDNA Clone DAZAP1-b

• Plasmid: pET28a(+) DAZAP1-b
• Parent Plasmid: pET28a original
• Expressed: His-DAZAP1-b
• Genbank: NM_018959
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG
CGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGAT
CCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTT
GACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGG
AGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCAACCAGTCTC
GAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTGGGGACGGTG
CTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGACCCCAAGCC
ATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGAT
GGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTC
GGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAA
GAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACGCCGAGAAGC
AGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTG
GACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGA
AGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAGC
CAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGC
TGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGG
AATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGACCGCCCCCTG
CAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCCTACATCGTG
TCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCCTCAGGGCTA
CGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCGC
CAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGG
GCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACAT
GAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTCAGTATGCAG
GTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCAGACCCCAGC
CAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTCGGGGGGCCC
CCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAAG
GGTTCCACCCCTACCGACGCTAGGGATCC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSNNSGADEIGKLFVGGL
DWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVGTV
LASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNKIFV
GGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDEQSV
DQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNAANG
WAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTSYIV
STPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPATPG
AAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFSDPS
QQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU4128
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal 6H tag