cDNA Clone DAZAP1-b

• Plasmid: pEBG6P-1 DAZAP1-b T269A T315A
• Parent Plasmid: pEBG6P
• Expressed: GST-DAZAP1-b T269A T315A
• Genbank: NM_018959
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTG
GGCGGTCTTGACTGGAGCACGACCCAAGAGACTCTGCGCAGCTACTTTTC
CCAATATGGAGAAGTCGTAGATTGTGTTATCATGAAAGATAAAACCACCA
ACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGACCCAAACTGTGTG
GGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATCGA
CCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGA
AGGAAGGATGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAG
ATATTTGTCGGTGGAATTCCTCACAATTGTGGTGAGACAGAGCTCAGGGA
ATACTTCAAGAAGTTCGGAGTGGTCACGGAGGTAGTCATGATCTATGACG
CCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACTTTCGAGGACGAA
CAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGCAA
AAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGC
CGGGACAGCCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCT
GCCAATGGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAGCAAGGATATGG
CCCGCAAGGAATGTGGGTGCCGGCAGGACAGGCGATTGGTGGCTATGGAC
CGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCACCGTTCACCTCC
TACATCGTGTCCGCCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTCCC
TCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCAC
CTCCACCGCCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCC
GCTCCCGGGGCAGCACCTCTGGCTTTCCCACCGCCTCCGTCTCAGGCTGC
CCCGGACATGAGCAAGCCCCCGACAGCTCAGCCAGACTTCCCCTATGGTC
AGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGACAGGGCTTCTCA
GACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGGTC
GGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACA
ACGTGCAAGGGTTCCACCCCTACCGACGCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSNNSGADEIGKLFV
GGLDWSTTQETLRSYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCV
GTVLASRPHTLDGRNIDPKPCTPRGMQPERTRPKEGWQKGPRSDNSKSNK
IFVGGIPHNCGETELREYFKKFGVVTEVVMIYDAEKQRPRGFGFITFEDE
QSVDQAVNMHFHDIMGKKVEVKRAEPRDSKSQAPGQPGASQWGSRVVPNA
ANGWAGQPPPTWQQGYGPQGMWVPAGQAIGGYGPPPAGRGAPPPPPPFTS
YIVSAPPGGFPPPQGFPQGYGAPPQFSFGYGPPPPPPDQFAPPGVPPPPA
APGAAPLAFPPPPSQAAPDMSKPPTAQPDFPYGQYAGYGQDLSGFGQGFS
DPSQQPPSYGGPSVPGSGGPPAGGSGFGRGQNHNVQGFHPYRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4057
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.