cDNA Clone SPI-1

• Plasmid: pGEX6P-1-SPI-1
• Parent Plasmid: pGEX6P1
• Expressed: GST-spi-1
• Genbank: X52056
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGAAGGGT
TTCCCCTCGTCCCCCCTCCATCAGAAGACCTGGTGCCCTATGACACGGAT
CTATACCAACGCCAAACGCACGAGTATTACCCCTATCTCAGCAGTGATGG
GGAGAGCCATAGCGACCATTACTGGGACTTCCACCCCCACCACGTGCACA
GCGAGTTCGAGAGCTTCGCCGAGAACAACTTCACGGAGCTCCAGAGCGTG
CAGCCCCCGCAGCTGCAGCAGCTCTACCGCCACATGGAGCTGGAGCAGAT
GCACGTCCTCGATACCCCCATGGTGCCACCCCATCCCAGTCTTGGCCACC
AGGTCTCCTACCTGCCCCGGATGTGCCTCCAGTACCCATCCCTGTCCCCA
GCCCAGCCCAGCTCAGATGAGGAGGAGGGCGAGCGGCAGAGCCCCCCACT
GGAGGTGTCTGACGGCGAGGCGGATGGCCTGGAGCCCAGGCCTGGGCTCC
TGCCTGGGGAGACAGGCAGCAAGAAGAAGATCCGCCTGTACCAGTTCCTG
TTGGACCTGCTCCGCAGCGGCGACATGAAGGACAGCATCTGGTGGGTGGA
CAAGGACAAGGGCACCTTCCAGTTCTCGTCCAAGCACAAGGAGGCGCTGG
CGCACCGCTGGGGCATCCAGAAGGGCAACCGCAAGAAGATGACCTACCAG
AAGATGGCGCGCGCGCTGCGCAACTACGGCAAGACGGGCGAGGTCAAGAA
GGTGAAGAAGAAGCTCACCTACCAGTTCAGCGGCGAAGTGCTGGGCCGCG
GGGGCCTGGCCGAGCGGCGCCACCCGCCCCACCACCATCACCATCACCAT
TAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEGFPLVPPPSEDLVPYDTD
LYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSV
QPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSP
AQPSSDEEEGERQSPPLEVSDGEADGLEPRPGLLPGETGSKKKIRLYQFL
LDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQ
KMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRHPPHHHHHHH
*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4032
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.