cDNA Clone IKK alpha

• Plasmid: pFBHTa IKK alpha P147R
• Parent Plasmid: pCR2.1
• Expressed: His-IKKa alpha P147R
• Genbank: BI551198
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGATCCGGAATTCGAGCGGCCCC
CGGGGCTGCGGCCGGGCGCGGGCGGGCCCTGGGAGATGCGGGAGCGGCTG
GGCACCGGCGGCTTCGGGAACGTCTGTCTGTACCAGCATCGGGAACTTGA
TCTCAAAATAGCAATTAAGTCTTGTCGCCTAGAGCTAAGTACCAAAAACA
GAGAACGATGGTGCCATGAAATCCAGATTATGAAGAAGTTGAACCATGCC
AATGTTGTAAAGGCCTGTGATGTTCCTGAAGAATTGAATATTTTGATTCA
TGATGTGCCTCTTCTAGCAATGGAATACTGTTCTGGAGGAGATCTCCGAA
AGCTGCTCAACAAACCAGAAAATTGTTGTGGACTTAAAGAAAGCCAGATA
CTTTCTTTACTAAGTGATATAGGGTCTGGGATTCGATATTTGCATGAAAA
CAAAATTATACATCGAGATCTAAAACGTGAAAACATAGTTCTTCAGGATG
TTGGTGGAAAGATAATACATAAAATAATTGATCTGGGATATGCCAAAGAT
GTTGATCAAGGAAGTCTGTGTACATCTTTTGTGGGAACACTGCAGTATCT
GGCCCCAGAGCTCTTTGAGAATAAGCCTTACACAGCCACTGTTGATTATT
GGAGCTTTGGGACCATGGTATTTGAATGTATTGCTGGATATAGGCCTTTT
TTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGATTAAGAAGAAGGA
TCCAAAGTGTATATTTGCATGTGAAGAGATGTCAGGAGAAGTTCGGTTTA
GTAGCCATTTACCTCAACCAAATAGCCTTTGTAGTTTAATAGTAGAACCC
ATGGAAAACTGGCTACAGTTGATGTTGAATTGGGACCCTCAGCAGAGAGG
AGGACCTGTTGACCTTACTTTGAAGCAGCCAAGATGTTTTGTATTAATGG
ATCACATTTTGAATTTGAAGATAGTACACATCCTAAATATGACTTCTGCA
AAGATAATTTCTTTTCTGTTACCACCTGATGAAAGTCTTCATTCACTACA
GTCTCGTATTGAGCGTGAAACTGGAATAAATACTGGTTCTCAAGAACTTC
TTTCAGAGACAGGAATTTCTCTGGATCCTCGGAAACCAGCCTCTCAATGT
GTTCTAGATGGAGTTAGAGGCTGTGATAGCTATATGGTTTATTTGTTTGA
TAAAAGTAAAACTGTATATGAAGGGCCATTTGCTTCCAGAAGTTTATCTG
ATTGTGTAAATTATATTGTACAGGACAGCAAAATACAGCTTCCAATTATA
CAGCTGCGTAAAGTGTGGGCTGAAGCAGTGCACTATGTGTCTGGACTAAA
AGAAGACTATAGCAGGCTCTTTCAGGGACAAAGGGCAGCAATGTTAAGTC
TTCTTAGATATAATGCTAACTTAACAAAAATGAAGAACACTTTGATCTCA
GCATCACAACAACTGAAAGCTAAATTGGAGTTTTTTCACAAAAGCATTCA
GCTTGACTTGGAGAGATACAGCGAGCAGATGACGTATGGGATATCTTCAG
AAAAAATGCTAAAAGCATGGAAAGAAATGGAAGAAAAGGCCATCCACTAT
GCTGAGGTTGGTGTCATTGGATACCTGGAGGATCAGATTATGTCTTTGCA
TGCTGAAATCATGGAGCTACAGAAGAGCCCCTATGGAAGACGTCAGGGAG
ACTTGATGGAATCTCTGGAACAGCGTGCCATTGATCTATATAAGCAGTTA
AAACACAGACCTTCAGATCACTCCTACAGTGACAGCACAGAGATGGTGAA
AATCATTGTGCACACTGTGCAGAGTCAGGACCGTGTGCTCAAGGAGCTGT
TTGGTCATTTGAGCAAGTTGTTGGGCTGTAAGCAGAAGATTATTGATCTA
CTCCCTAAGGTGGAAGTGGCCCTCAGTAATATCAAAGAAGCTGACAATAC
TGTCATGTTCATGCAGGGGAAAAGGCAGAAAGAAATATGGCATCTCCTTA
AAATTGCATGTACACAGAGTTCTGCCCGGTCCCTTGTAGGATCCAGTCTA
GAAGGTGCAGTAACCCCTCAGACATCAGCATGGCTGCCCCCGACTTCAGC
AGAACATGATCATTCTCTGTCATGTGTGGTAACTCCTCAAGATGGGGAGA
CTTCAGCACAAATGATAGAAGAAAATTTGAACTGCCTTGGCCATTTAAGC
ACTATTATTCATGAGGCAAATGAGGAACAGGGCAATAGTATGATGAATCT
TGATTGGAGTTGGTTAACAGAATGA

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFERPPGLRPGAGGPWEMRERL
GTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHA
NVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQI
LSLLSDIGSGIRYLHENKIIHRDLKRENIVLQDVGGKIIHKIIDLGYAKD
VDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPF
LHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLIVEP
MENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSA
KIISFLLPPDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQC
VLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPII
QLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLIS
ASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHY
AEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQL
KHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDL
LPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSL
EGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLS
TIIHEANEEQGNSMMNLDWSWLTE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4018
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.