cDNA Clone Speg (mouse)

• Plasmid: pGEX6P-3 Speg (mouse) Y1606-F1859 (NP_031489 numbering)
• Parent Plasmid: pGEX6P3
• Expressed: GST-Speg (mouse) Y1606-F1859 (NP_031489 numbering)
• Genbank: NP_031489
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCTACGACATCCACCAAGAGATTGGCAGGGGTGCCTTCTCCTACCT
GCGGCGTGTAGTGGAGAGGAGCTCTGGCCTGGAGTTTGCAGCCAAGTTCA
TCCCTAGCCAGGCTAAGCCTAAAGCATCAGCAAGACGCGAGGCGCGCTTG
CTGGCCCGGCTCCAGCATGGCTGTGTGCTCTACTTCCACGAAGCTTTTGA
GAGGCGCAGGGGACTTGTCATAGTCACGGAGCTCTGCACAGAAGAGCTTC
TAGAGCGCATGGCCAGGAAGCCCACCGTGTGTGAGTCTGAGACTCGGACC
TATATGCGTCAAGTGCTAGAGGGGATATGCTATCTGCATCAGAGCCATGT
GCTGCACCTGGATGTCAAGCCGGAGAACCTGCTTGTGTGGGATGGTGCAG
GGGGTGAAGAGCAGGTGCGCATCTGTGACTTCGGGAACGCCCAGGAACTC
ACTCCGGGAGAGCCCCAGTACTGTCAGTATGGCACGCCAGAGTTTGTAGC
ACCTGAGATTGTCAACCAGAGCCCTGTGTCTGGAGTCACTGACATCTGGC
CCGTGGGCGTCGTTGCCTTTCTCTGTTTGACGGGCATCTCCCCATTTGTT
GGGGAGAATGACCGGACAACACTCATGAATATCCGAAACTACAACGTGGC
ATTTGAGGAGACTACGTTCCTGAGTCTGAGCAGGGAGGCCCGGGGCTTCC
TCATCAAAGTGCTGGTGCAGGATCGACTGAGACCTACTGCAGAAGAGACA
TTGGAACATCCTTGGTTCTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSYDIHQEIGRGAFSYLRRVV
ERSSGLEFAAKFIPSQAKPKASARREARLLARLQHGCVLYFHEAFERRRG
LVIVTELCTEELLERMARKPTVCESETRTYMRQVLEGICYLHQSHVLHLD
VKPENLLVWDGAGGEEQVRICDFGNAQELTPGEPQYCQYGTPEFVAPEIV
NQSPVSGVTDIWPVGVVAFLCLTGISPFVGENDRTTLMNIRNYNVAFEET
TFLSLSREARGFLIKVLVQDRLRPTAEETLEHPWF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36494
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 55768 Daltons