cDNA Clone PRMT5

• Synonyms: HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs
• Plasmid: pGEX6P-3-PRMT5
• Parent Plasmid: pGEX6P3
• Expressed: GST PRMT5
• Genbank: NM_006109.3 GI:88900510
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTC
CAGCGGGAGGGACCTGAATTGCGTCCCCGAAATAGCTGACACACTAGGGG
CTGTGGCCAAGCAGGGGTTTGATTTCCTCTGCATGCCTGTCTTCCATCCG
CGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCCGGTCC
CCAGACACGATCAGACCTACTGCTGTCAGGAAGGGACTGGAATACGCTAA
TTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAAAGTGGAGAAG
ATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGC
ATATTTGGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACA
CCAACCTGGCCAGAGTTTTGACCAACCACATCCACACTGGCCATCACTCT
TCCATGTTCTGGATGCGGGTACCCTTGGTGGCACCAGAGGACCTGAGAGA
TGATATAATTGAGAATGCACCAACTACACACACAGAGGAGTACAGTGGGG
AGGAGAAAACGTGGATGTGGTGGCACAACTTCCGGACTTTGTGTGACTAT
AGTAAGAGGATTGCAGTGGCTCTTGAAATTGGGGCTGACCTCCCATCTAA
TCATGTCATTGATCGCTGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCC
CCACTAGCATTTTCCTGACCAATAAGAAGGGATTTCCTGTTCTTTCTAAG
ATGCACCAGAGGCTCATCTTCCGGCTCCTCAAGTTGGAGGTGCAGTTCAT
CATCACAGGCACCAACCACCACTCAGAGAAGGAGTTCTGCTCCTACCTCC
AATACCTGGAATACTTAAGCCAGAACCGTCCTCCACCTAATGCCTATGAA
CTCTTTGCCAAGGGCTATGAAGACTATCTGCAGTCCCCGCTTCAGCCACT
GATGGACAATCTGGAATCTCAGACATATGAAGTGTTTGAAAAGGACCCCA
TCAAATACTCTCAGTACCAGCAGGCCATCTATAAATGTCTGCTAGACCGA
GTACCAGAAGAGGAGAAGGATACCAATGTCCAGGTACTGATGGTGCTGGG
AGCAGGACGGGGACCCCTGGTGAACGCTTCCCTGCGGGCAGCCAAGCAGG
CCGACCGGCGGATAAAGCTGTATGCTGTGGAGAAAAACCCAAATGCCGTG
GTGACGCTAGAGAACTGGCAGTTTGAAGAATGGGGAAGCCAAGTGACCGT
AGTCTCATCAGACATGAGGGAATGGGTGGCTCCAGAGAAAGCAGACATCA
TTGTCAGTGAGCTTCTGGGCTCATTTGCTGACAATGAATTGTCGCCTGAG
TGCCTGGATGGAGCCCAGCACTTCCTAAAAGATGATGGTGTGAGCATCCC
CGGGGAGTACACTTCCTTTCTGGCTCCCATCTCTTCCTCCAAGCTGTACA
ATGAGGTCCGAGCCTGTAGGGAGAAGGACCGTGACCCTGAGGCCCAGTTT
GAGATGCCTTATGTGGTACGGCTGCACAACTTCCACCAGCTCTCTGCACC
CCAGCCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATGATTGACA
ACAACCGCTATTGCACCTTGGAATTTCCTGTGGAGGTGAACACAGTACTA
CATGGCTTTGCCGGCTACTTTGAGACTGTGCTTTATCAGGACATCACTCT
GAGTATCCGTCCAGAGACTCACTCTCCTGGGATGTTCTCATGGTTTCCCA
TCCTCTTCCCTATTAAGCAGCCCATAACGGTACGTGAAGGCCAAACCATC
TGTGTGCGTTTCTGGCGATGCAGCAATTCCAAGAAGGTGTGGTATGAGTG
GGCTGTGACAGCACCAGTCTGTTCTGCTATTCATAACCCCACAGGCCGCT
CATATACCATTGGCCTCTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAMAVGGAGGSRVSSGRD
LNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRS
DLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGL
PAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIE
NAPTTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVID
RWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGT
NHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNL
ESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPEEEKDTNVQVLMVLGAGRG
PLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSD
MREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYT
SFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCF
TFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRP
ETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTA
PVCSAIHNPTGRSYTIGL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36336
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 99505 Daltons