cDNA Clone p300 (mouse)

• Plasmid: pcDNA5-FRT/TO-FLAG2 (mouse) p300
• Parent Plasmid: pcDNA5 FRT/TO FLAG2
• Genbank: NM_177821.6 GI:123173876*
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGGCCGAGAATGTGGTGGAACCCGGGCCGCCTTCAGCCAA
GCGGCCTAAACTCTCATCTCCGGCCCTCTCGGCGTCCGCCAGCGATGGCA
CAGATTTTGGTTCACTGTTTGACCTGGAACATGACTTACCAGATGAATTA
ATCAACTCTACAGAATTGGGACTAACCAATGGTGGCGATATCAGTCAGCT
TCAGACAAGTCTTGGCATAGTACAAGATGCAGCCTCGAAACATAAACAGC
TGTCAGAACTGCTGAGGTCTGGTAGCTCCCCAAACCTCAACATGGGAGTC
GGTGGCCCAGGCCAAGCGATGGCCAGCCAGGCCCAACAGAACAGCCCTGG
ATTAAGTTTGATAAATAGCATGGTCAAAAGCCCAATGGCACAGACAGGCT
TGACTTCTCCAAACATGGGGATTGGCAGTAGTGGACCAAATCAGGGTCCT
ACTCAGTCCCCAGCAGGTATGATGAACAGTCCAGTGAACCAGCCTGCCAT
GGGAATGAACACAGGGATGAATGCTGGCATGAATCCTGGAATGTTGGCTG
CAGGCAATGGACAAGGGATAATGCCCAATCAAGTCATGAACGGTTCCATT
GGAGCAGGCCGGGGACGGCCAAACATGCAGTACCCAAATGCAGGCATGGG
CAATGCTGGCAGTTTATTGACTGAGCCACTACAGCAGGGCTCTCCTCAGA
TGGGAGGACAGCCAGGATTGAGAGGCCCCCAACCACTTAAGATGGGAATG
ATGAACAATCCCAGTCCTTATGGTTCACCATACACTCAGAATTCTGGACA
GCAGATTGGAGCAAGTGGCCTTGGTCTCCAAATTCAGACAAAGACTGTTC
TACCAAATAACTTATCTCCATTTGCAATGGACAAAAAGGCAGTTCCTGGT
GGGGGAATGCCCAGTATGGGCCAGCAGCCTACCCCATCGGTCCAGCAGCC
AGGCCTGGTGACTCCAGTTGCCGCAGGAATGGGTTCTGGAGCACACACAG
CTGATCCAGAGAAGCGCAAGCTCATCCAGCAGCAGCTTGTTCTCCTTTTA
CATGCTCACAAGTGCCAGCGCCGGGAGCAAGCTAATGGGGAAGTGAGGCA
GTGCAACCTTCCTCACTGTCGTACCATGAAAAATGTCCTAAACCATATGA
CACATTGCCAGTCAGGCAAATCCTGCCAAGTGGCACATTGTGCATCTTCT
CGACAAATCATTTCACACTGGAAAAATTGCACAAGGCATGATTGTCCTGT
GTGTCTTCCTCTCAAAAATGCTGGGGATAAGCGAAATCAACAGTCAATTT
TGACTGGAGCACCAGTTGGGCTTGGAAACCCTAGCTCTCTAGGAGTGGGG
CAGCAGTCCACTCCTAGCCTAAGCACTGTTAGTCAGATTGACCCCAGCTC
TATAGAGCGAGCTTACGCTGCTCTTGGACTACCCTATCAAGTAAACCAGA
TTCCACCACAACCCCAGGTACAAGCAAAGAATCAACAAAGCCAGCCATCT
GGACAGTCTCCCCAGGGCATGCGGTCTGTGAACAACATGAGTGCTAGTCC
TATGGGTGTAAATGGAGGTGTTGGGGTTCAGACGCCAAATCTTCTTTCTG
ACTCCATGTTGCATTCAACTATAAATTCTCAAAACCCAATGATGAGTGAA
AATGCTGGTGTGGCCTCCCTGGGTCCTTTGCCAACAGCAGCTCAGCCATC
TAGTACTGGAATTCGAAAACAGTGGCATGAAGATATTACTCAGGATCTTC
GAAACCACCTTGTTCATAAACTTGTTCAAGCCATATTTCCCACTCCGGAT
CCTGCTGCTTTAAAAGACCGACGGATGGAAAATCTTGTTGCATATGCTCG
TAAAGTGGAAGGGGACATGTATGAATCTGCAAACAATAGAGCGGAATACT
ATCACCTCCTAGCCGAGAAGATCTATAAGATCCAGAAGGAACTAGAAGAG
AAACGAAGGACTAGACTACAGAAACAGAACATGCTACCGAATGCTCCTGG
CATGGGTCCAGTTCCTATGAATACTGGGTCGAACATGGAGCAGCAACCAA
CAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGC
AGTGTGCCGAACCACATGATGCCTCGGATGACTCCACAGCCTGGTTTGAA
TCAATTTGGGCAGATGAATATGCCACAGCCCCCTATTGGACCCCGGCAAC
CCTCTCCTCTTCAGCACCATGGACAATTGGCTCAGTCTGGGTCACTCAAT
CCGCCTATGGGCTATGGACCTCGCATGCAGCAGGCTTCCGGCCAGAACCA
GTTCCTCTCCCAGACTCAGTTCACATCCCAAGGAATGAATGTAACAAACA
TGCCTTTGGCTCCATCCAGCGGTCAAGCACCAGTGTCTCAAGCACAAATG
TCCAGTTCTTCTTGCCCAGTGAACTCTCCTATAATGCCTCCAGGGTCTCA
AGGGAGCCACATTCACTGTCCTACTCTGCCTCAACAAGCTCATCAGAATT
CACCTTCTCCTGTTCCTAGCCGCACCCCTACTCCTCATCATACCCCCCCA
AGCATAGGGAATCAACCACCACCAGCAACAGCAATTCCAACACCTGTTCC
TACACCTCCTGCCATACCACCTGGACCACAGCCCCCATCTCTGCATCCCT
CATCTAGACAAACACCTACACCACCAACACATCTGCCTCCCCAAGTGCAG
CCTTCACTTCCTGCTGCTCCTTCTGCTGACCAGTCCCAGCAACAGCCTCG
GTCACAGCAGAGCACAGCAGTTTCTGTTCCTACCCCAACAGCACCATTGC
TGCCTCCTCAGCCTTCCACTCCGCTTTCTCAGCCAGCTGTGAGCATTGAA
GGCCAGGTCTCAAACCCTCCATCTACTAGTAGCACCGAAGTGAATTCTCA
GACCATTCCTGAGAAGCAGCCTTCACAGGAAGTGAAAATGGAGTCTAAAA
TGGAGGTGGATAAGCCAGAACCAGCAGATGCTCAACCTGAGGATACAAAG
GAGGCTAAAGGTGAGGATGTTAAAGTAGAACCTACAGAAATGGAGGAGAG
AGGCCCTGAGTTAAAAACTGATGGGAAAGAGGAGGAAGAACAGCCAAGCA
CCTCTGCTACCCAGTCCTCCCCAGCTCCTGGACAGTCAAAGAAGAAGATT
TTCAAACCAGAGGAATTACGACAGGCTCTGATGCCAACATTGGAGGCACT
TTACCGGCAGGACCCAGAATCTCTTCCCTTTCGTCAGCCTGTGGACCCGC
AGCTTCTAGGAATCCCTGATTACTTTGATATTGTGAAGAGCCCCATGGAT
CTTTCTACAATCAAGAGGAAGTTAGATACTGGACAATACCAAGAGCCCTG
GCAATATATAGATGATATTTGGCTTATGTTCAACAATGCCTGGTTATATA
ACCGTAAAACTTCAAGGGTATACAAATATTGCTCTAAACTTTCTGAAGTA
TTTGAACAAGAAATTGACCCTGTCATGCAAAGCCTTGGATACTGTTGTGG
CAGAAAGTTGGAGTTTTCTCCACAGACTCTTTGTTGCTATGGGAAACAGT
TATGCACAATCCCCCGTGATGCTACTTACTACAGTTACCAGAACAGGTAT
CATTTCTGTGAGAAGTGTTTCAATGAAATCCAAGGGGAGAGCGTTTCTTT
GGGTGATGACCCTTCCCAACCTCAAACTACAATAAATAAAGAACAGTTTT
CCAAGAGAAAAAATGACACGTTGGATCCTGAACTGTTTGTTGAGTGCACA
GAGTGTGGAAGAAAGATGCACCAGATCTGTGTCCTTCACCACGAGATCAT
CTGGCCATCTGGGTTTGTCTGTGATGGCTGTTTAAAGAAAACTGCACGAA
CTAGGAAAGAAAATAAGCTTTCTGCTAAAAGATTGCCATCTACCAGACTT
GGGACCTTTCTGGAGAATCGAGTGAATGACTTTCTGAGGCGACAAAATCA
CCCTGAATCAGGAGAGGTCACTGTTCGGGTTGTTCATGCTTCTGACAAAA
CTGTGGAAGTGAAACCAGGCATGAAAGCAAGGTTTGTAGATAGTGGAGAG
ATGGCAGAATCTTTTCCATACCGAACAAAGGCCCTGTTTGCCTTTGAAGA
AATTGATGGTGTTGACTTGTGTTTCTTCGGCATGCATGTTCAAGAATATG
GCTCTGACTGCCCCCCTCCCAACCAGAGGAGGGTATACATATCTTACCTC
GATAGTGTTCATTTCTTCCGTCCTAAATGCTTGCGGACTGCAGTCTATCA
TGAAATTCTAATTGGATATTTGGAATATGTCAAGAAATTAGGATACACAA
CAGGGCACATCTGGGCCTGTCCACCCAGTGAAGGGGATGACTATATCTTC
CATTGCCATCCACCTGATCAGAAGATACCAAAGCCCAAGCGCCTGCAAGA
ATGGTACAAAAAGATGCTTGACAAGGCTGTATCAGAACGTATTGTCCATG
ACTACAAGGATATTTTAAAACAAGCTACTGAAGATCGATTAACAAGTGCA
AAGGAACTACCCTACTTTGAAGGTGATTTCTGGCCCAATGTTCTAGAAGA
AAGCATCAAGGAGCTTGAACAGGAAGAGGAAGAGAGGAAACGGGAAGAGA
ACACCAGCAACGAGAGTACTGATGTAACAAAAGGAGACAGCAAAAATGCT
AAGAAGAAGAATAACAAGAAAACCAGCAAAAACAAGAGCAGCCTGAGTAG
GGGCAATAAGAAGAAGCCTGGTATGCCCAATGTGTCTAATGACCTTTCTC
AGAAACTGTATGCCACCATGGAAAAGCATAAAGAGGTTTTCTTTGTGATC
CGCCTCATCGCTTGTCCTGCTCCCAATTCCCTGCCTCCCATTGTTGATCC
TGACCCTCTCATCCCCTGTGACCTGATGGACGGTAGAGATGCATTTCTCA
CCCTTGCAAGGGACAAGCACCTGGAGTTCTCTTCACTTCGAAGAGCCCAG
TGGTCTACCATGTGCATGCTGGTGGAGCTCCATACACAGAGTCAGGATCG
CTTTGTCTACACCTGTAACGAGTGCAAGCACCACGTGGAGACACGCTGGC
ATTGCACTGTGTGTGAGGATTATGACCTGTGCATCACTTGTTATAACACT
AAAAATCATGACCACAAAATGGAGAAACTAGGCCTTGGCTTGGATGATGA
GAGCAATAACCAGCAAGCTGCAGCTACACAGAGCCCAGGAGACTCCCGCC
GCCTGAGCATCCAACGCTGCATCCAGTCTCTGGTGCATGCCTGTCAGTGC
CGCAACGCCAACTGCTCCCTGCCTTCCTGCCAGAAGATGAAGAGGGTTGT
GCAGCACACCAAAGGCTGCAAACGGAAAACCAATGGTGGGTGCCCCATTT
GCAAACAGCTTATTGCCCTCTGCTGCTATCATGCCAAGCACTGCCAGGAG
AACAAGTGCCCGGTGCCATTTTGCCTCAACATCAAGCAAAAGCTCCGGCA
GCAACAGCTGCAGCACCGGCTCCAACAGGCTCAGATGCTCCGTAGGAGGA
TGGCCAGCATGCAACGAACTGGTGTGGCGGGGCAGCAGCAGGGCCTGCCG
TCCCCAACTCCTGCTACACCAACTACCCCTACTGGCCAACAGCCAGCCAC
CCCACAGACACCCCAGCCCCAACCCACCTCTCAACCACAGCCCACTCCTC
CCAACAACATGACACCCTACTTACCCAGGACTCAGACTACTGGCCCTGTG
TCCCAGGGTAAGGCACCAGGCCAGGTGACCCCACCTACCCCACCTCAGAC
TGCTCAGGCCCCCCTTCCAGGGCCTCCACCTGCAGCAGTAGAAATGGCAA
TGCAAATTCAGAGGGCAGCAGAGACACAGCGCCAGATGGCCCATGTACAG
ATTTTCCAAAGGCCAATCCAGCACCAGATGCCCCAGATGTCACCCATGGC
CCCTATGGGCATGAACCCACCTCCTATGGCCAGAGGTCCTGGTGGGCATT
TGGATCCAGGAATAGGTCCAGCAGGAATGCAGCAACAGCCACCTTGGGCC
CAAGGAGGAATGCCTCAGCCCCAGCAGATGCAGTCAGGGATGCCAAGGCC
AGCCATGATGTCAGTGGCCCAGCATGGTCAGCCTTTAAACATGGCTCCAC
AACCAGGATTGGGCCAAGTGGGTGTGAGCCCTCTCAAGCCAGGCACTGTG
TCTCAACAAGCCTTACAAAACCTTTTGCGGACTCTCAGGTCTCCCAGTTC
TCCCTTACAGCAGCAACAGGTGCTTAGTATCCTTCATGCCAACCCCCAAC
TGTTGGCTGCATTCATCAAGCAGCGGGCTGCCAAGTATGCCAACCCTAAT
CCACAGCCTCTCCCTGGACAGCCTGGCATGACCCAGGGACAGCCAGGGCT
GCAGCCACCTACCATGCCTGGCCAGCAAGGTGTCCACTCCAACCCAGCCT
TGCAGAACATGAATCCTCTGCAGGCAGGTGTCCAGAGAGCTGGCCTGCCC
CAGCAGCAGCCGCAGCAGCAGCTCCAGCCACCCATGGGAGCAATGAGTCC
CCAAGCTCAGCAAATGAACATGAATCACAATACCATGCCTTCACAGTTCA
GAGACATCTTAAGACGGCAGATGATGCAACAGCAGGGAGCAGGGCCAGGA
ATCGGCCCTGGAATGGCCAACCAGTTCCAGCAGCCCCAAGGAATTGGCTA
TCCACCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGAATGCAGCATCACA
TGCAGCAAATGCAGCAAGGAAATATGGGACAAATGGGTCAGCTTCCCCAG
GCTCTGGGGGCTGAGGCAGGAGCCAGTCTACAGGCCTATCAGCAGCGACT
TCTTCAACAACAGATGGGGTCTCCTGCTCAGCCCAACCCTATGAGCCCAC
AGCAGCACATGCTCCCAAATCAGGCACAGTCCCCACACCTACAAGGTCAA
CAAATCCCTAATTCTCTCTCCAATCAAGTGCGCTCTCCCCAGCCTGTCCC
TTCTCCTCGACCACAGTCTCAGCCCCCCCATTCCAGCCCATCTCCGAGGA
TGCAGCCTCAGCCTTCTCCACACCATGTTTCTCCACAGACCAGTTCCCCA
CATCCTGGACTGGTAGCTGCCCAGGCTGCCAACCCTATGGAACAAGGGCA
TTTTGCCAGCCCAGACCAGAATTCAATGCTTTCACAGCTCGCTAGCAATC
CTGGCATGGCAAACCTCCACGGGGCAAGTGCCACGGACCTGGGACTGAGC
AGCGATAATGCAGACTTGAATTCAAACCTCTCACAGAGTACACTAGACAT
ACACTAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMAENVVEPGPPSAKRPKLSSPALSASAS
DGTDFGSLFDLEHDLPDELINSTELGLTNGGDISQLQTSLGIVQDAASKH
KQLSELLRSGSSPNLNMGVGGPGQAMASQAQQNSPGLSLINSMVKSPMAQ
TGLTSPNMGIGSSGPNQGPTQSPAGMMNSPVNQPAMGMNTGMNAGMNPGM
LAAGNGQGIMPNQVMNGSIGAGRGRPNMQYPNAGMGNAGSLLTEPLQQGS
PQMGGQPGLRGPQPLKMGMMNNPSPYGSPYTQNSGQQIGASGLGLQIQTK
TVLPNNLSPFAMDKKAVPGGGMPSMGQQPTPSVQQPGLVTPVAAGMGSGA
HTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLN
HMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQ
SILTGAPVGLGNPSSLGVGQQSTPSLSTVSQIDPSSIERAYAALGLPYQV
NQIPPQPQVQAKNQQSQPSGQSPQGMRSVNNMSASPMGVNGGVGVQTPNL
LSDSMLHSTINSQNPMMSENAGVASLGPLPTAAQPSSTGIRKQWHEDITQ
DLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRA
EYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAPGMGPVPMNTGSNMEQ
QPTGMTTNGPVPDPSMIRGSVPNHMMPRMTPQPGLNQFGQMNMPQPPIGP
RQPSPLQHHGQLAQSGSLNPPMGYGPRMQQASGQNQFLSQTQFTSQGMNV
TNMPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQGSHIHCPTLPQQAH
QNSPSPVPSRTPTPHHTPPSIGNQPPPATAIPTPVPTPPAIPPGPQPPSL
HPSSRQTPTPPTHLPPQVQPSLPAAPSADQSQQQPRSQQSTAVSVPTPTA
PLLPPQPSTPLSQPAVSIEGQVSNPPSTSSTEVNSQTIPEKQPSQEVKME
SKMEVDKPEPADAQPEDTKEAKGEDVKVEPTEMEERGPELKTDGKEEEEQ
PSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEALYRQDPESLPFRQPV
DPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEPWQYIDDIWLMFNNAW
LYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYG
KQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKE
QFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPSGFVCDGCLKKT
ARTRKENKLSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHAS
DKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQ
EYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLG
YTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERI
VHDYKDILKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKR
EENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSND
LSQKLYATMEKHKEVFFVIRLIACPAPNSLPPIVDPDPLIPCDLMDGRDA
FLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVET
RWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDDESNNQQAAATQSPGD
SRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGC
PICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLR
RRMASMQRTGVAGQQQGLPSPTPATPTTPTGQQPATPQTPQPQPTSQPQP
TPPNNMTPYLPRTQTTGPVSQGKAPGQVTPPTPPQTAQAPLPGPPPAAVE
MAMQIQRAAETQRQMAHVQIFQRPIQHQMPQMSPMAPMGMNPPPMARGPG
GHLDPGIGPAGMQQQPPWAQGGMPQPQQMQSGMPRPAMMSVAQHGQPLNM
APQPGLGQVGVSPLKPGTVSQQALQNLLRTLRSPSSPLQQQQVLSILHAN
PQLLAAFIKQRAAKYANPNPQPLPGQPGMTQGQPGLQPPTMPGQQGVHSN
PALQNMNPLQAGVQRAGLPQQQPQQQLQPPMGAMSPQAQQMNMNHNTMPS
QFRDILRRQMMQQQGAGPGIGPGMANQFQQPQGIGYPPQQQQQQQQQRMQ
HHMQQMQQGNMGQMGQLPQALGAEAGASLQAYQQRLLQQQMGSPAQPNPM
SPQQHMLPNQAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPS
PRMQPQPSPHHVSPQTSSPHPGLVAAQAANPMEQGHFASPDQNSMLSQLA
SNPGMANLHGASATDLGLSSDNADLNSNLSQSTLDIH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31272
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_177821.6 CDS:; extra gcagcagca after nt6647 (extra QQQ after residue 2222) g2030a (G677E); c5007t (silent); a5133g (silent); All derive from EST; ; Estimated MW of expressed protein : 266029 Daltons