cDNA Clone PTPN1

• Plasmid: pGEX6P-2-PTPN1
• Parent Plasmid: pGEX6P2
• Expressed: GST-PTPN1
• Genbank: NM_002827.2 GI:18104977
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGATGGAAAAGGAGTTCGAGCAGATCGACAAGTCCGGGAG
CTGGGCGGCCATTTACCAGGATATCCGACATGAAGCCAGTGACTTCCCAT
GTAGAGTGGCCAAGCTTCCTAAGAACAAAAACCGAAATAGGTACAGAGAC
GTCAGTCCCTTTGACCATAGTCGGATTAAACTACATCAAGAAGATAATGA
CTATATCAACGCTAGTTTGATAAAAATGGAAGAAGCCCAAAGGAGTTACA
TTCTTACCCAGGGCCCTTTGCCTAACACATGCGGTCACTTTTGGGAGATG
GTGTGGGAGCAGAAAAGCAGGGGTGTCGTCATGCTCAACAGAGTGATGGA
GAAAGGTTCGTTAAAATGCGCACAATACTGGCCACAAAAAGAAGAAAAAG
AGATGATCTTTGAAGACACAAATTTGAAATTAACATTGATCTCTGAAGAT
ATCAAGTCATATTATACAGTGCGACAGCTAGAATTGGAAAACCTTACAAC
CCAAGAAACTCGAGAGATCTTACATTTCCACTATACCACATGGCCTGACT
TTGGAGTCCCTGAATCACCAGCCTCATTCTTGAACTTTCTTTTCAAAGTC
CGAGAGTCAGGGTCACTCAGCCCGGAGCACGGGCCCGTTGTGGTGCACTG
CAGTGCAGGCATCGGCAGGTCTGGAACCTTCTGTCTGGCTGATACCTGCC
TCTTGCTGATGGACAAGAGGAAAGACCCTTCTTCCGTTGATATCAAGAAA
GTGCTGTTAGAAATGAGGAAGTTTCGGATGGGGCTGATCCAGACAGCCGA
CCAGCTGCGCTTCTCCTACCTGGCTGTGATCGAAGGTGCCAAATTCATCA
TGGGGGACTCTTCCGTGCAGGATCAGTGGAAGGAGCTTTCCCACGAGGAC
CTGGAGCCCCCACCCGAGCATATCCCCCCACCTCCCCGGCCACCCAAACG
AATCCTGGAGCCACACAATGGGAAATGCAGGGAGTTCTTCCCAAATCACC
AGTGGGTGAAGGAAGAGACCCAGGAGGATAAAGACTGCCCCATCAAGGAA
GAAAAAGGAAGCCCCTTAAATGCCGCACCCTACGGCATCGAAAGCATGAG
TCAAGACACTGAAGTTAGAAGTCGGGTCGTGGGGGGAAGTCTTCGAGGTG
CCCAGGCTGCCTCCCCAGCCAAAGGGGAGCCGTCACTGCCCGAGAAGGAC
GAGGACCATGCACTGAGTTACTGGAAGCCCTTCCTGGTCAACATGTGCGT
GGCTACGGTCCTCACGGCCGGCGCTTACCTCTGCTACAGGTTCCTGTTCA
ACAGCAACACATAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEMEKEFEQIDKSGSWAAI
YQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINA
SLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSL
KCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETR
EILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGI
GRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRF
SYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEP
HNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTE
VRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVL
TAGAYLCYRFLFNSNT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31200
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 76789 Daltons