cDNA Clone CDK9

• Plasmid: pFBDual GST-6P-1 CDK9/Cyclin T1
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-CDK9/ Cyclin T1
• Genbank: NM_001240.2 GI:17978465* NM_001261
• Tag: GST
• Species: Human

Sequence of Insert

DNA sequence of Cyclin T1 insert

CCATGGAGGGAGAGAGGAAGAACAACAACAAACGGTGGTATTTCACTCGA
GAACAGCTGGAAAATAGCCCATCCCGTCGTTTTGGCGTGGACCCAGATAA
AGAACTTTCTTATCGCCAGCAGGCGGCCAATCTGCTTCAGGACATGGGGC
AGCGTCTTAACGTCTCACAATTGACTATCAACACTGCTATAGTATACATG
CATCGATTCTACATGATTCAGTCCTTCACACAGTTCCCTGGAAATTCTGT
GGCTCCAGCAGCCTTGTTTCTAGCAGCTAAAGTGGAGGAGCAGCCCAAAA
AATTGGAACATGTCATCAAGGTAGCACATACTTGTCTCCATCCTCAGGAA
TCCCTTCCTGATACTAGAAGTGAGGCTTATTTGCAACAAGTTCAAGATCT
GGTCATTTTAGAAAGCATAATTTTGCAGACTTTAGGCTTTGAACTAACAA
TTGACCACCCACATACTCATGTAGTAAAGTGCACTCAACTTGTTCGAGCA
AGCAAGGACTTAGCACAGACTTCTTACTTCATGGCAACCAACAGCCTGCA
TTTGACCACATTTAGCCTGCAGTACACACCTCCTGTGGTGGCCTGTGTCT
GCATTCACCTGGCTTGCAAGTGGTCCAATTGGGAGATCCCAGTCTCAACT
GACGGGAAGCACTGGTGGGAGTATGTTGACGCCACTGTGACCTTGGAACT
TTTAGATGAACTGACACATGAGTTTCTACAGATTTTGGAGAAAACTCCCA
ACAGGCTCAAACGCATTTGGAATTGGAGGGCATGCGAGGCTGCCAAGAAA
ACAAAAGCAGATGACCGAGGAACAGATGAAAAGACTTCAGAGCAGACAAT
CCTCAATATGATTTCCCAGAGCTCTTCAGACACAACCATTGCAGGTTTAA
TGAGCATGTCAACTTCTACCACAAGTGCAGTGCCTTCCCTGCCAGTCTCC
GAAGAGTCATCCAGCAACTTAACCAGTGTGGAGATGTTGCCGGGCAAGCG
TTGGCTGTCCTCCCAACCTTCTTTCAAACTAGAACCTACTCAGGGTCATC
GGACTAGTGAGAATTTAGCACTTACAGGAGTTGATCATTCCTTACCACAG
GATGGTTCAAATGCATTTATTTCCCAGAAGCAGAATAGTAAGAGTGTGCC
ATCAGCTAAAGTGTCACTGAAAGAATACCGCGCGAAGCATGCAGAAGAAT
TGGCTGCCCAGAAGAGGCAACTGGAGAACATGGAAGCCAATGTGAAGTCA
CAATATGCATATGCTGCCCAGAATCTCCTTTCTCATCATGATAGCCATTC
TTCAGTCATTCTAAAAATGCCCATAGAGGGTTCAGAAAACCCGGAGCGGC
CTTTTCTGGAAAAGGCTGACAAAACAGCTCTCAAAATGAGAATCCCAGTG
GCAGGTGGAGATAAAGCTGCGTCTTCAAAACCAGAGGAGATAAAAATGCG
CATAAAAGTCCATGCTGCAGCTGATAAGCACAATTCTGTAGAGGACAGTG
TTACAAAGAGCCGAGAGCACAAAGAAAAGCACAAGACTCACCCATCTAAT
CATCATCATCATCATAATCACCACTCACACAAGCACTCTCATTCCCAACT
TCCAGTTGGTACTGGGAACAAACGTCCTGGTGATCCAAAACATAGTAGCC
AGACAAGCAACTTAGCACATAAAACCTATAGCTTGTCTAGTTCTTTTTCC
TCTTCCAGTTCTACTCGTAAAAGGGGACCCTCTGAAGAGACTGGAGGGGC
TGTGTTTGATCATCCAGCCAAGATTGCCAAGAGTACTAAATCCTCTTCCC
TAAATTTCTCCTTCCCTTCACTTCCTACAATGGGTCAGATGCCTGGGCAT
AGCTCAGACACAAGTGGCCTTTCCTTTTCACAGCCCAGCTGTAAAACTCG
TGTCCCTCATTCGAAACTGGATAAAGGGCCCACTGGGGCCAATGGTCACA
ACACGACCCAGACAATAGACTATCAAGACACTGTGAATATGCTTCACTCC
CTGCTCAGTGCCCAGGGTGTTCAGCCCACTCAGCCTACTGCATTTGAATT
TGTTCGTCCTTATAGTGACTATCTGAATCCTCGGTCTGGTGGAATCTCCT
CGAGATCTGGCAATACAGACAAACCCCGGCCACCACCTCTGCCATCAGAA
CCTCCTCCACCACTTCCACCCCTTCCTAAGTAAGGTACC

DNA sequence of CDK9 insert
GGATCCATGGCAAAGCAGTACGACTCGGTGGAGTGCCCTTTTTGTGATGA
AGTTTCCAAATACGAGAAGCTCGCCAAGATCGGCCAAGGCACCTTCGGGG
AGGTGTTCAAGGCCAGGCACCGCAAGACCGGCCAGAAGGTGGCTCTGAAG
AAGGTGCTGATGGAAAACGAGAAGGAGGGGTTCCCCATTACAGCCTTGCG
GGAGATCAAGATCCTTCAGCTTCTAAAACACGAGAATGTGGTCAACTTGA
TTGAGATTTGTCGAACCAAAGCTTCCCCCTATAACCGCTGCAAGGGTAGT
ATATACCTGGTGTTCGACTTCTGCGAGCATGACCTTGCTGGGCTGTTGAG
CAATGTTTTGGTCAAGTTCACGCTGTCTGAGATCAAGAGGGTGATGCAGA
TGCTGCTTAACGGCCTCTACTACATCCACAGAAACAAGATCCTGCATAGG
GACATGAAGGCTGCTAATGTGCTTATCACTCGTGATGGGGTCCTGAAGCT
GGCAGACTTTGGGCTGGCCCGGGCCTTCAGCCTGGCCAAGAACAGCCAGC
CCAACCGCTACACCAACCGTGTGGTGACACTCTGGTACCGGCCCCCGGAG
CTGTTGCTCGGGGAGCGGGACTACGGCCCCCCCATTGACCTGTGGGGTGC
TGGGTGCATCATGGCAGAGATGTGGACCCGCAGCCCCATCATGCAGGGCA
ACACGGAGCAGCACCAACTCGCCCTCATCAGTCAGCTCTGCGGCTCCATC
ACCCCTGAGGTGTGGCCAAACGTGGACAACTATGAGCTGTACGAAAAGCT
GGAGCTGGTCAAGGGCCAGAAGCGGAAGGTGAAGGACAGGCTGAAGGCCT
ATGTGCGTGACCCATACGCACTGGACCTCATCGACAAGCTGCTGGTGCTG
GACCCTGCCCAGCGCATCGACAGCGATGACGCCCTCAACCACGACTTCTT
CTGGTCCGACCCCATGCCCTCCGACCTCAAGGGCATGCTCTCCACCCACC
TGACGTCCATGTTCGAGTACTTGGCACCACCGCGCCGGAAGGGCAGCCAG
ATCACCCAGCAGTCCACCAACCAGAGTCGCAATCCCGCCACCACCAACCA
GACGGAGTTTGAGCGCGTCTTCTGAGGATCC

Amino Acid Sequence

Amino acid sequence of expressed Cyclin T1
MEGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQ
RLNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKK
LEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTI
DHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVC
IHLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPN
RLKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLM
SMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHR
TSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEEL
AAQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERP
FLEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSV
TKSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQ
TSNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSL
NFSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHN
TTQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISS
RSGNTDKPRPPPLPSEPPPPLPPLPK*

Amino acid sequence of expressed GST-CDK9. PreScission site underlined. M1 of CDK9 in bold.
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAKQYDSVECPFCDEVSKY
EKLAKIGQGTFGEVFKARHRKTGQKVALKKVLMENEKEGFPITALREIKI
LQLLKHENVVNLIEICRTKASPYNRCKGSIYLVFDFCEHDLAGLLSNVLV
KFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLITRDGVLKLADFG
LARAFSLAKNSQPNRYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCIM
AEMWTRSPIMQGNTEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVK
GQKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDP
MPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFE
RVF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU31050
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Note: Sequence differences compared with NM_001240.2 GI:17978465: t453c, c2034t, c1341g (all silent); ; Dual expression vector for expression of untagged Cyclin T1 and GST-CDK9 in insect cells.; ; pSC-A Cyclin T1 (DU31023) (RT-PCR from Human Brain Total RNA) pEBG2T CDK9 (DU1494), pFastBacDual GST6P-1 Cyclin T1 (DU31036)