cDNA Clone slit-robo-GAP 1

• Plasmid: pcDNA5-FRT/TO-FLAG slit-robo-GAP 1 isoform 2 D90-M1062(end)
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG srGAP1-2 D90-M1062(end)
• Genbank: AB037725*
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGACCAGAACCTGTTGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGCAACCTTGAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGATAAGTGAGGATTCTACCAGGATGTTTAAAAAGAGCAAAGAGATTGCATTCCAACTTCATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGGTGATGAAAACATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGCTGAAAGAGGCCGAAAAACAAGAG
GAAAAGCAAATTGGGAGATCTGGTGATCCAGTCTTCCATATTCGACTAGA
GGAGAGACATCAACGGCGAAGCTCTGTAAAGAAAATTGAAAAAATGAAAG
AAAAAAGACAAGCAAAATATTCAGAAAATAAGCTAAAATCAATTAAGGCA
CGGAACGAATATCTCCTAACACTTGAAGCCACCAATGCCTCAGTTTTCAA
GTACTATATTCATGATCTTTCTGATTTAATTGATTGCTGTGATCTTGGCT
ACCATGCAAGTCTGAACAGAGCCCTAAGAACATATCTGTCTGCGGAGTAC
AACCTTGAAACCTCCAGACATGAGGGCTTAGACATTATTGAGAATGCAGT
TGATAATTTAGAGCCCAGGAGCGATAAGCAGAGATTCATGGAGATGTACC
CTGCTGCGTTCTGTCCACCAATGAAGTTTGAGTTTCAGTCTCACATGGGT
GATGAGGTGTGCCAGGTCAGTGCCCAGCAGCCAGTCCAGGCAGAGCTCAT
GCTCAGGTACCAACAGTTGCAGTCCCGCCTTGCCACGCTCAAAATCGAGA
ATGAAGAGGTTAAGAAAACGACTGAAGCCACCTTGCAGACGATACAAGAT
ATGGTCACCATCGAGGACTATGATGTTTCTGAATGCTTCCAGCACAGTCG
TTCCACAGAATCTGTGAAGTCCACTGTCTCTGAAACCTACCTGAGTAAAC
CCAGCATCGCCAAGAGAAGAGCCAACCAGCAGGAAACTGAACAGTTCTAC
TTCATGAAACTCAGAGAATATTTGGAAGGCAGTAATCTCATCACAAAACT
TCAAGCCAAACATGACTTGCTGCAGAGGACCCTGGGAGAAGGTCATAGAG
CTGAATATATGACTACAAGCCGAGGACGAAGAAACTCGCACACAAGACAT
CAGGACTCAGGACAGGTTATTCCCCTCATTGTGGAAAGCTGTATTCGGTT
CATCAATCTCTATGGTCTTCAGCATCAGGGGATTTTCAGAGTGTCTGGTT
CCCAGGTGGAAGTCAATGATATTAAAAATTCATTTGAGAGAGGTGAAAAT
CCTTTGGCTGATGACCAGAGTAACCATGATATTAACTCAGTTGCTGGCGT
TCTGAAGCTCTATTTCCGTGGGCTGGAAAACCCCCTCTTTCCTAAGGAAA
GATTTAACGATCTGATTTCTTGTATCAGAATAGATAATCTCTATGAGAGG
GCGCTTCACATCCGCAAACTCCTCCTGACTTTGCCCAGGTCGGTCCTTAT
AGTGATGAGGTACCTCTTTGCCTTCCTCAATCATCTATCACAGTACAGCG
ATGAGAATATGATGGACCCTTATAACCTGGCCATTTGCTTTGGCCCAACA
TTGATGCCTGTCCCAGAAATACAGGATCAAGTGTCTTGCCAGGCACATGT
GAATGAAATTATCAAAACCATCATCATCCACCATGAGACTATTTTCCCAG
ATGCTAAAGAGCTGGATGGCCCTGTTTATGAGAAATGTATGGCTGGAGAT
GACTATTGCGACAGCCCATACAGTGAGCACGGTACATTGGAGGAAGTGGA
CCAAGATGCTGGTACAGAGCCCCACACAAGTGAAGATGAATGTGAGCCAA
TAGAAGCAATAGCCAAGTTTGACTATGTTGGGCGGTCCGCCAGAGAACTA
TCCTTCAAGAAGGGTGCCTCCCTGCTGCTGTATCACCGTGCATCTGAGGA
CTGGTGGGAAGGCAGGCACAACGGGATTGACGGGCTGGTGCCTCACCAGT
ATATAGTGGTGCAGGATATGGATGATACGTTTTCAGACACTCTGAGCCAA
AAAGCCGACAGTGAGGCCAGCAGTGGGCCAGTCACGGAAGACAAGTCCTC
ATCCAAGGACATGAACTCCCCGACAGACCGTCATCCTGACGGCTATTTAG
CCAGGCAACGAAAAAGAGGAGAGCCACCCCCTCCAGTAAGGCGTCCTGGC
AGGACCAGTGATGGCCATTGCCCGCTCCACCCTCCACATGCCCTTTCTAA
CTCCTCAGTTGACCTAGGGTCCCCAAGCCTTGCCAGTCACCCCCGGGGCC
TGCTGCAGAACCGTGGCCTCAACAATGACAGTCCTGAGCGGAGGCGCAGG
CCTGGCCATGGCAGCCTGACCAACATCAGCCGGCACGACTCCCTCAAGAA
GATCGACAGCCCTCCCATTAGAAGGTCCACGTCATCAGGGCAATACACGG
GCTTCAATGACCACAAGCCACTGGACCCAGAGACAATTGCTCAGGATATT
GAAGAAACGATGAACACAGCTTTGAATGAACTCCGAGAACTGGAGAGACA
GAGCACAGCAAAGCATGCCCCTGATGTGGTGCTGGATACCCTGGAGCAAG
TGAAAAACTCTCCCACCCCTGCCACTTCCACGGAATCTCTCAGCCCTTTG
CACAACGTTGCCCTCAGGAGCTCCGAGCCTCAGATTCGACGTAGCACGAG
CTCCTCCAGTGACACAATGAGTACTTTCAAGCCTATGGTGGCACCCAGAA
TGGGCGTGCAGCTGAAGCCTCCAGCCCTTAGGCCAAAACCTGCTGTTCTT
CCAAAAACAAATCCTACCATAGGACCTGCCCCACCTCCCCAGGGTCCAAC
AGACAAGTCATGCACAATGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMR
FMQISEDSTRMFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISA
ESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAK
YSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLN
RALRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCP
PMKFEFQSHMGDEVCQVSAQQPVQAELMLRYQQLQSRLATLKIENEEVKK
TTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKR
RANQQETEQFYFMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTT
SRGRRNSHTRHQDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVN
DIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLI
SCIRIDNLYERALHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMD
PYNLAICFGPTLMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKELD
GPVYEKCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAK
FDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIVVQD
MDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRHPDGYLARQRKR
GEPPPPVRRPGRTSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQNRG
LNNDSPERRRRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHK
PLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPT
PATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQLK
PPALRPKPAVLPKTNPTIGPAPPPQGPTDKSCTM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16971
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. This clone derived from clone that starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…