cDNA Clone Nudel (NDEL1-PLSV)

• Plasmid: pETBlue2.6His Nudel
• Parent Plasmid: pETBlue2 6His
• Expressed: 6His Nudel
• Genbank: AY004871.1 GI:12043568
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGATGGTGAAGATATACCAGATTTTTCAAGTTTAAAGGAGGAAACTGCTTATTGGAAGGAACTTTCCTTGAAGTATAAGCAAAGCTTCCAGGAAGCTCGGGATGAGCTAGTTGAATTCCAGGAAGGAAGCAGAGAATTAGAAGCAGAGTTGGAGGCACAATTAGTACAGGCTGAACAAAGAAATAGAGACTTGCAGGCTGATAACCAAAGACTGAAATATGAAGTGGAGGCATTAAAGGAGAAGCTAGAGCATCAATATGCACAGAGCTATAAGCAGGTCTCAGTGTTAGAAGATGAT
TTAAGTCAGACTCGGGCCATTAAGGAGCAGTTGCATAAGTATGTGAGAGA
GCTGGAGCAGGCCAACGACGACCTGGAGCGAGCCAAAAGGGCAACAATAG
TTTCACTGGAAGACTTTGAACAAAGGCTAAACCAGGCCATTGAACGAAAT
GCATTTTTAGAAAGTGAACTTGATGAAAAGGAATCTTTGTTGGTCTCTGT
ACAGAGGTTAAAGGATGAAGCAAGAGATTTAAGGCAAGAACTAGCAGTTC
GGGAAAGACAACAGGAAGTAACTAGAAAGTCGGCTCCTAGCTCTCCAACT
CTAGACTGTGAAAAGATGGACTCCGCCGTCCAAGCATCACTTTCTTTGCC
AGCTACCCCTGTTGGCAAAGGAACGGAGAACACTTTTCCTTCACCGAAAG
CTATACCAAATGGTTTTGGTACCAGTCCACTAACTCCCTCTGCTAGGATA
TCAGCACTAAACATCGTGGGGGATCTCTTACGGAAAGTAGGGGCTTTAGA
ATCCAAATTAGCAGCTTGCAGGAATTTTGCAAAGGACCAAGCATCACGAA
AATCCTATATTTCAGGGAATGTTAACTGTGGGGTGCTGAATGGCAATGGC
ACAAAGTTCTCTCGATCAGGGCATACATCTTTCTTCGACAAAGGGGCAGT
AAACGGCTTTGACCCCGCTCCTCCTCCTCCTGGTCTGGGCTCCTCGCGTC
CATCGTCAGCGCCGGGTATGCTGCCTCTCAGTGTGTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSMDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVE
FQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQ
SYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDF
EQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQE
VTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGF
GTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISG
NVNCGVLNGNGTKFSRSGHTSFFDKGAVNGFDPAPPPPGLGSSRPSSAPG
MLPLSV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16930
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct.